锥虫

从锥虫可变表面糖蛋白（VSG）中发现反式剪接以来，已经发现很多低等真核生物体内都存在这种剪接形式。

RNA trans-splicing in many primary eukaryotes has been found , and testified , since it was discovered from splicing of RNA of variable surface glycoprotein ( VSG ) in trypanosome .

利用血吸虫、锥虫、旋毛虫、肺吸虫、肝片吸虫、囊虫阳性血清与PAPS弓形虫抗体诊断液进行凝集试验，结果均呈阴性，未见发生交叉反应；

It wasn 't found that PAPS diagnosis reagent for T. gondii 's antibody elicited the immune response with the positive serum of Schistosoma , Trypanosome , Trichina , Lung fluke , Fasciola hepatica , Bladder worm .

伊氏锥虫微环DNA的PCR扩增

Amplification of the kinetoplast DNA minicircles of Trypanosoma evansi by PCR

用毛细管电泳检测伊氏锥虫微环DNAPCR扩增产物

Detect PCR product of mini - circle DNA of Trypanosoma evansi by capillary electrophoresis

水牛伊氏锥虫间接ELISA检测方法的建立

Establishment of an Indirect ELISA for Trypanosoma evansi in Water Buffaloes

克隆至大肠杆菌，用PCR鉴定及锥虫蓝培养基筛选阳性克隆；

Finally , positive clones were screened on trypan blue culture medium and expressed in DH5 α .

伊氏锥虫cDNA表达文库的构建

Construction of Trypanosoma evansi cDNA expression library

证明我国不同伊氏锥虫在动基DNA序列上存在一些差别，这可能由于点突变的缘故。

It was proved that minor difference existed in KDNA sequences among variant Trypanosoma evansi strains in China , as could be explained by point mutant .

伊氏锥虫HGPRT基因的RT-PCR扩增及克隆

RT PCR Amplification and Cloning of HGPRT Gene of Trypanosoma evansi

结论LPS能够促进心肌肌球蛋白特异的自身免疫反应及锥虫抗原诱导的自身免疫性心肌炎。

It is concluded LPS can promote myosin-specific autoimmune responses and the autoimmune myocarditis induced by T. cruzi antigen .

抗伊氏锥虫VAT兔源抗体库的建立及其抗虫效果检测

Establishment of rabbit anti - trypanosome evansi antibody bank and its detection efficacy against trypanosome evansi infection

结果：分选所得LC纯化率为84.48%，锥虫蓝染色显示细胞活性>95%。

Results : The purification rate of Langerhans cells was 84.48 % and the cell viability was more than 95 % .

RT-PCR法分离伊氏锥虫VSG基因的研究

Isolation of VSG gene of Trypanosoma evansi by RT-PCR

分离主要依赖宿主血细胞、血小板和锥虫之间表面电荷的差异；成功的关键是缓冲液PH的标准状态和离子强度。

The separation depends fundamentally on surface charge difference between the host blood cells , platelets and trypanosomes , and the most important factor was the PH and ionic strength of buffer .

甲基吡喃的合成布氏锥虫葡糖基磷脂酰肌醇（GPI）的研究进展

Research prospects on glycosylphosphatidylinositol ( gpi ) of Trypanosoma brucei

方法应用锥虫蓝排染法、生长曲线测定法、克隆形成实验检测药物对HL-60细胞生长的影响；

Methods Cell inhibitory effect were determined by trypan blue dye exclusion test , growth curve method and colony formation .

以布氏锥虫亮氨酰tRNA合成酶为靶标的抗锥虫药物筛选系统的建立

The Establishment of an Anti-Trypanosoma Drug Screening System with Leucyl-tRNA Synthetase as an Inhibition Target

抗伊氏锥虫云南水牛株VSG、VAT抗体库建立及抗虫效果比较研究

Establishment of Anti-VSG or Anti-VAT Antibody Banks and Their Efficacy Against Trypanosoma Evansi Infection in SD Rats

对于寄生虫布氏锥虫CTP合成酶的研究使研究者离成功治愈非洲嗜睡病又近了一步。

Studies of the enzyme CTP synthetase in the parasite Trypanosoma brucei have brought researchers closer to a cure for African sleeping sickness .

克隆伊氏锥虫2个VSG的分离纯化和电泳分析

Isolation and Electrophoresis Analysis on Two VSG of a Cloned Trypanosoma evansi

伊氏锥虫体外培养株的建立及其HGPRT活性测定

Establishment of Trypanosoma evansi in vitro Cultivation Strain and Determination of Its HGPRT Activities

伊氏锥虫VSG抗原库的建立及其免疫保护效果检测

The Establishment of VSG Antigenic Reservoir of Trypanosome Evansi and Its Detection of Protective Immunity

分别于照射后3、24、48h行锥虫蓝染色，平行孔内的细胞进行胰酶消化后，用Coulter计数器计算细胞数量，应用流式细胞术测定细胞周期。

At each time point as indicated after the exposure , the cells in parallel wells were trypsinized for cell counting using Coulter counter .

方法：采用流式细胞仪、噻唑蓝（简称MTT）法和锥虫蓝染色法对猫儿眼植物酸抗肿瘤细胞作用进行分析。

METHODS : The flow cytometry , methyl thiazolyl tetrazolium ( MTT ) and trypan blue staining were used to analyze the effect of mao er yan plant acid on inhabitation of tumor cells .

伊氏锥虫HGPRT缺陷株和自然株的HGPRT基因的分离、克隆与比较

Isolation , cloning and comparison of HGPRT gene of natural and HGPRT - strains of Trypanosoma evansi

布氏锥虫中的SUMO系统在细胞有丝分裂期姐妹染色单体分离过程中具有重要作用，并且是维持布氏锥虫基因组稳定所必须的。

SUMO system is essential for sister-chromatin segregation in mitosis , and plays key roles in maintaining genome integrity in T. brucei .

利用18SRRNA基因序列分析方法，对我国的湖北、广西、新疆和浙江四省的伊氏锥虫及一株布氏锥虫进行分子分类学研究。

The phylogenetic relation of trypanosoma sp in Hubei , guangxi , xinjiang , Zhejiang province and a strain of trypanosoma bruce were analyzed by using 18S rRNA sequence .

目的研究不同免疫途径对马疫锥虫动基体DNA（kDNA）诱导正常小鼠产生抗双链DNA（dsDNA）抗体及其致病性的影响。

Objective To study the effect of different immunization methods on the production and pathogenicity of anti-dsDNA antibodies induced by kinetoplast DNA ( kDNA ) derived from trypanosoma equiperdum ( TE ) on normal mice .

布氏冈比亚锥虫（T.b.g.）可见于非洲西部和中部。

Trypanosoma brucei gambiense ( T.b.g. ) is found in west and central Africa .

在报告的昏睡病病例中，布氏冈比亚锥虫（T.b.g.）引起的病例占95%。

Trypanosoma brucei gambiense ( T.b.g. ) accounts for95 % of reported cases of sleeping sickness .