钙黄绿素

光谱法研究钙黄绿素与鲱鱼精DNA的相互作用

Study on the Interaction between Calcein and Herring Sperm DNA by Spectrometry

方法：采用钙黄绿素（calein）和1，8－ANS荧光物质包载于脂质体内，采用荧光分光光度法，通过测定荧光强度，确定渗漏率。

Methods : The calcein and ANS were encapsulated into liposome respectively . The rate of leakage was determined by measuring the fluorometric intensity .

钙黄绿素-藏红T与DNA作用机理的光谱研究

Spectrophotometric Studies on the Interaction Mechanism of Calcein - Safranine T Fluorescence Pair with DNA

基于β-环糊精-丙酮能加速与增敏Al-钙黄绿素的荧光反应，建立了流动注射荧光法测定中草药中微量Al的新体系（Al-Calcein-β-CD-丙酮）。

β - CD-Acetone can speed up and enhance the fluorescence reaction of Al-calcein .

碘化物/钙黄绿素染色检测细胞凋亡，MTT法测定神经元生存能力。

The survival ability of neurons was tested by MTT .

应用钙黄绿素-藏红T为荧光探针，建立了新的DNA测定方法。

Using Calcein-Safranine T as a new donor-acceptor pair , a novel fluorescence energy transfer system for the rapid determination of DNA was developed .

利用藏红T-钙黄绿素能量转移荧光法测定痕量的Cr（Ⅵ），提高了测定铬的灵敏度.FRET技术及其在蛋白质-蛋白质分子相互作用研究中的应用

So this system of fluorescence resonance energy transfer is applied to the determination of Cr (ⅵ) . Fluorescence Resonance Energy Transfer Assay : Applications in The Study of Protein-protein Interaction

结果：EDTA滴定中，钙黄绿素法较钙紫红素法更具有灵敏性、准确性、可靠性。

Results : The experiment shows the calcein method in EDTA titrations has the advantage of sensity , high accuracy , and precision to Eriochrome Blue black R ( EBBR ) .

方法用PAF处理培养的野生型和PSD93基因敲除型的小鼠皮质神经元；经碘化物（PI）/钙黄绿素（Calcein）染色，荧光显微镜观察并计算细胞死亡率（%）；

Methods PAF treated the cultural cortex neurons in wild type and PSD 93 knockout mice and the cells were stained with PI / Calcein AM for apoptosis ;

牛血清白蛋白与钙黄绿素反应制备白蛋白纳米粒（BSANP）

BSA was reacted with calcein to prepare BSA nanoparticles ( BSANP )

钙黄绿素-AM染色存活的RGCs，结果显示RGCs细胞体较大，多数细胞有长度大于细胞体直径2倍的突起。

The results of calcein-AM staining , which stained the living cells only , showed large cell body of RGCs and most of RGCs had a protuberance whose length was twice longer than the diameter of the cells .

钙黄绿素蓝荧光猝灭测定茶叶中的锰

Fluorescence Quenching of Calcein Blue for the Determination of Manganese (ⅱ) in Tea

钙黄绿素-Al~（3+）荧光光谱法测定F~-

Fluorometric Determination of F ~ - with Calcein - Al ~ ( 3 + )

结论：石膏的含量测定应采用钙黄绿素法。

Conclusion : We should use the calcein methods in the calcium content determination for Gypsum .

结论：应用钙黄绿素和茜素红染色可以对骨微破裂进行荧光染色，在激光扫描共聚焦显微镜下能够观察到清晰的骨微破裂图像。

CONCLUSION : Bone microcrack can be stained with calcein and alizarin and observed clearly under LSCM .

通过测定叶酸脂质体对钙黄绿素的包封率和渗漏率，考察叶酸脂质体的稳定性。

The envelopment and leakage rate of folate liposome to calcein were determined to evaluate the stability of folate liposome . 3 .

所有荧光染料中，以钙黄绿素染色后观察效果为佳，在波长为522nm的激光扫描下发出绿色荧光。

Calcein staining was the best selective method to identify microcracks and the green fluorescence was observed under 522 nm laser wavelength .

配合物的形成可高效敏化Yb~（3+）离子的近红外发光，同时使配体钙黄绿素的自身荧光显著猝灭。

The visible fluorescence from Calcein was quenched when complex forms , while the near-infrared fluorescence from Yb ( III ) was greatly sensitized .

如果设计出环引物，并优化钙黄绿素显色剂中锰离子与钙黄绿素的比例，可以进一步提升扩增效率，在临床应用中发挥更大的作用。

The amplification efficiency will be enhanced if we have designed the loop primers and optimized the manganese ion and calcein in the calcein chromogenic reagent .

稀土Er~（3+）离子在低浓度时对Yb~（3+）-钙黄绿素体系的近红外发光也有一定的增敏作用，它的加入同样会猝灭钙黄绿素及其配合物的荧光。

It was also noted that the near-infrared fluorescence from Yb ( III ) could be enhanced by addition of small amount of Er3 + ions .

方法以钙黄绿素脂质体为模型，考察了糖脂比、磷脂组成以及叔丁醇含量对钙黄绿素滞留率的影响。

Methods The influences of sugar-lipid mass ratio , lipid composition and TBA content on the retention of calcein inside liposomes before and after freeze-drying were investigated .

荧光显微镜和原子力显微镜观察结果证实，制备的叶酸脂质体分布均匀，大小一致，所包封钙黄绿素的荧光强度一致。

As the fluorescence microscope and atomic force microscopy showed , the prepared liposomes had uniform folate distribution and size , fluorescence intensity of the enveloped calcein was almost the same .

毛细流自组装成环荧光显微测定血清样品中硫酸奎尼丁镉-钙黄绿素配合物在玻片表面的毛细流成环组装及其数值化荧光成象分析研究

Determination of Quinidine Sulfate in Serum Samples with Self-ordered Ring Technique by Capillary Flow Effect Ordered Ring Assembly of Cadmium-Calcein Complex Directed Under the Capillary Flow on the Glass Slide Supports and Its Digitalized Fluorescence Image Analysis