重组蛋白质

类弹性蛋白（elastin-likepolypeptide，ELP）一种新型的重组蛋白质分离纯化标签。

Elastin-like polypeptide ( ELP ) is a new recombinant protein purification tag .

重组蛋白质是指利用DNA重组技术生产的蛋白质，重组蛋白的菌种构建至发酵、纯化的工艺研究是重组蛋白研发和产业化的重要研究内容。

Recombinant protein refers to the use of recombinant DNA techniques in the production of the protein , whose species construct to fermentation , purification technology research development and industrialization of the recombinant protein is the important research content .

结果表明，试验构建的DNA疫苗和重组蛋白质疫苗都能诱导产生针对JEV的特异性体液免疫和细胞免疫应答。

Datas showed that both the recombinant protein and the DNA vaccine can induce humoral and cellular immune responses .

coli中的表达来获得蛋白质类药物，已经成为生产基因工程药物的重要手段，然而重组蛋白质在E。

Coli have been one of important methods to produce genetic engineering pharmaceuticals , but the recombinant proteins are accumulated as inclusion bodies in E. coli .

SDS聚丙烯酰胺凝胶电泳和凝胶扫描分析，AB基因表达的蛋白质分子质量约为225ku，其重组蛋白质表达量占菌体总蛋白质的29%，表达产物经亲和层析纯化后蛋白质纯度达96%。

SDS PAGE and scan analysis show the AB molecular mass is 22 5 ku and recombinant protein amounted to 29 % of the total bacterial protein . The AB purity amounted to 96 % through affinity chromatography .

分子伴侣、低分子量添加物等在复性过程中的应用及新的复性方法的建立都大大提高了重组蛋白质复性产率表达的rPA占菌体总蛋白的40%，以包涵体形式存在。

Renaturation yield of recombinant protein have been improved by using some additives , such as molecular chaperone , small molecules that interfere with intermolecular interactions responsible for aggregation .

重组蛋白质如RECQL；

Proteins involved in recombination such as RECQL ;

重组蛋白质纯化中切割蛋白酶的选择与应用进展

Application and Selection of Cleavage Protease in Purification of Recombinant Protein

利用这一系统，约30%的重组蛋白质能得到完全复性。

Accordingly , about 30 % reconstruction protein was refolded perfectly .

基因重组蛋白质的表达系统与分离纯化研究进展

Progress on the Expression System and Purification of Recombinant Proteins

植物生物反应器生产药用重组蛋白质的研究进展

Progress of Production of Pharmaceutical Recombinant Proteins with Plant Bioreactors

大肠杆菌异源重组蛋白质的变性与复性

Denaturation and renaturation of alien recombinant proteins in E.coli

动物细胞表达的基因重组蛋白质药物

Gene recombinant protein drug produced by animal cell line

利用这一系统可以得到与天然蛋白序列一致的重组蛋白质。

This system has been used for the generation of recombinant native proteins .

对基因重组蛋白质复性的哲学思考

Philosophic Thoughts on the Renaturation of Gene Recombinant Protein

重组蛋白质亲和标签的选择与串联亲和纯化的应用进展

Choosing of Affinity Tags for Recombinant Proteins and Application Progress of Tandem Affinity Purification

重组蛋白质纯化技术

The Progress of Recombinant Protein Purification Techniques

重组蛋白质主导着生物药品市场；

Recombination of proteins leads biopharmaceutical market ;

重组蛋白质技术作为一种方便的蛋白质生产来源，日益得到广泛运用。

Recombinant protein technology , as a convenient protein production source , is wildly used .

表面活性剂辅助重组蛋白质复性

Surfactant assisted refolding of recombinant proteins

该药是一种重组蛋白质，叫第8因子，能有效地帮助血液凝固。

The drug is a recombinant protein , called Factor VIII , which is necessary to help blood clot .

方法合成肽文库筛选，定向点突变，原核细胞重组蛋白质表达及受体-配体结合实验。

Methods Screening of synthetic peptide library , site directed mutagenesis , Flag-2 fusion protein expression in E.coli and cell-free-binding-assay .

两类方法指导我们选择重组蛋白质的最佳复性条件，并给了我们许多有益的哲学思考。

At the same time , the research on protein refoking has gave us many enlightenments in the thoughts of philosophy .

方法根据人工分子伴侣对重组蛋白质的复性机理，采用人工分子伴侣方法应用于重组内抑素的复性。

Methods Based on the reactive principle of molecular chaperone , we adopted an artificial molecular chaperone-assisted refolding method for recombinant endostatin refolding .

近年来，随着对包涵体体外复性机制的深入研究，从包涵体中复性重组蛋白质已经有了很多的策略和方法。

Recently , with the development of studying in mechanism of protein refolding , some new strategies were reported to solve this problem .

介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。

The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years .

分子医药农业是利用转基因植物为载体，以农业生产的方式规模化生产各种有治疗用途的重组蛋白质及多肽。

Molecular pharming is to use plant cells as bioreactors to produce various valuable proteins or peptides at large scales for pharmaceutical and nutritional uses .

包涵体中富含表达的重组蛋白质，它们经分离、洗涤、变性溶解以及复性等过程才能得到具有生物活性的蛋白质。

These inclusion bodies should be washed with denaturation buffer firstly , then followed by denaturation and refolding in proper buffer to harvest the active proteins .

采用蛋白质分子量标准物和纯化的重组蛋白质进行半干式蛋白质印迹，探讨半干式电泳印迹的多种因素对印迹效果的影响。

To elucidate the effect of the semi-dry electrophoretic transfer and its influencing factors in Western blot , the protein marker and purified recombination protein were used to analyzed .

描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性，综述了国内外变性、复性的有效方法。

This paper describe the formation , isolation , denaturation and renaturation of recombinant proteins as inclusion bodies in E. coli , and summarize the most efficient ways to refold recombinant proteins .