酚氧化酶

中国对虾（PENAEUSCHINENSIS）酚氧化酶的分离纯化及其部分生物化学性质

Purification and Partial Biochemical Characterization of Phenoloxidase from Penaeus chinensis

1995年7&9月，以养殖中国对虾为材料，实验室用弧菌菌悬液注射感染对虾，血清中测得一定量的酚氧化酶（PO）。

From July to September 1995 , phenoloxidase ( PO ) activity was measured after cultured shrimps were challenged by Vibrio SPP .

pH对酶活性的影响会在一定条件下抑制纤维二糖酶而促进酚氧化酶。

PH would promote the phenol oxidase activity and inhibit the cellobiase activity in certain condition .

血细胞中无酚氧化酶（PO）活性。

There were no activities of PO in haemocytes .

目的鉴定大黄粉虫幼虫体内与酚氧化酶（PO）诱导的黑色素合成相关的蛋白（MAPs）。

Objective To identify the proteins associated with PO-induced melanin synthesis ( MAPs ) in Tenebrio molitor larvae .

日本（虫寻）（Charybdisjaponica）血蓝蛋白的分离纯化及其酚氧化酶样生物化学性质的研究

Purification of Hemocyanin from Charybdis Japonica and Biochemical Properties of Hemocyanin Derived Phenoloxidase-Like Enzyme

短蛸（Octopusocellatus）酚氧化酶的分离纯化及其部分生物化学性质的研究

Purification and Partial Characterization of Phenoloxidase from Gray Octopus Ocellatus

结果表明：盐度、pH突变对2种养殖对虾抗菌活力、酚氧化酶活力的影响是显著的（P<0.05）。

The results show that the influence of sudden changes of salinity and pH on the antibacterial activity and phenoloxidase activity of two species of shrimps are very remarkable ( P < 0.05 ) .

酚氧化酶（phenoloxidase，PO）在昆虫中参与黑化包被、伤口愈合、表皮硬化、色素形成等过程。

Insect phenoloxidase ( PO ) participates in melanotic encapsulation , wound healing , cuticle sclerotization and pigment forming .

结果：两性霉素B合并氟康唑组的新生隐球菌酚氧化酶活性较空白对照组明显降低（P<0001），但在大于和接近最低抑菌浓度上限的组间无明显差异。

RESULTS : The phenoloxidase activity of amphotericin B and fluconazole group was lower than control group ( P < 0 001 ) . But there wasn 't significant difference when amphotericin B and fluconazole concentrations reached or exceeded the MICs .

酚氧化酶（phenoloxidase，简称PO，EC1.14.18.1，在哺乳动物中也称为酪氨酸酶）是昆虫生命过程中的重要调节酶。

Phenol oxidase ( PO , EC 1.14.18.1 , and was also known as tyrosinase in mammalian ) is one of the key enzymes in the development of insects .

饲料中的se和VE对酚氧化酶（PO）、溶菌酶、凝集效价和血清总蛋白含量等体液免疫指标都有显著的影响。

Dietary selenium and vitamin E separately had significant effects on all humoral immune indices , including PO , lysozyme , and agglutinin activity and serum total protein concentration .

含葡聚糖饲料能够显著提高凡纳滨对虾的血浆酚氧化酶活力与血浆蛋白浓度（P0.05），但对其它各项指标的影响均不显著。

Dietary β - 1 , 3 / 1 , 6 - glucan could significantly improve the Plasma Phenoloxidase Activity and Plasma Protein Concentration ( P0.05 ), but have no significant effects on other parameters .

目的观察日本血吸虫成虫酚氧化酶（phenoloxidase，PO）的酶谱及其活性。

Objective To observe the zymogram of phenoloxidase ( PO ) from the adult worms of S. japonicum and to mensurate its relative activity .

Cu是甲壳动物体内必需的微量营养素，不仅是酚氧化酶、超氧化物歧化酶活性中心的重要因子，也是合成血蓝蛋白的必需成分，但是高浓度的铜亦会对生物体会产生毒害作用。

Copper is a essential micronutrients of the crustaceans . It is not only a phenoloxidase and superoxide dismutase activity center factor , but also an essential component of hemocyanin . However , high concentrations of copper will produce toxic effects on crustacean .

对虾的血细胞数、溶菌（LSZ）活力、抗菌活力、超氧化物歧化酶（SOD）和酚氧化酶（PO）均显著高于对照池（P<0.05）；

The number of blood cell , the activity of phenoloxidase , superoxide dismutase ( SOD ), antibacterial enzyme and lysozyme were significantly higher ( P < 0.05 ) than the control pond .

CMC酶6d后急剧分泌，第7天达峰值，之后又急速下降；愈创木酚氧化酶和多酚氧化酶活性高峰出现最晚，均在第14天。

The peak of CMCase ( CMC : carboxymethyl cellulose ) activity appeared on the 7th day while enzyme production of guaiacol oxidase and polyphenol oxidase reached the peak on the 14th day .

河蟹摄食试验饲料1周后，0.1%组和0.5%组的酚氧化酶（PO）活性显著升高（P<0.05），但β-葡聚糖含量提高至1%以上时PO活性有下降的趋势；

After 1 week feeding , the phenoloxidase ( PO ) of 0.1 % group and 0.5 % group was increased markedly ( P < 0.05 ) . However decrease tendency was appearing , when the β - glucan content was above 1 % ;

本研究以家蚕（Bombyxmori）表皮为材料，进行酚氧化酶分离纯化和酶学性质研究，可为研究昆虫表皮生成、免疫以及以该酶为靶标新型害虫抑制剂提供理论依据。

In this study , silkworm ( Bombyx mori ) cuticle for material phenoloxidase isolation , identification and characterization studies , can be generated for the study of insect cuticle , immunization and the target enzyme for inhibitors of new pests and provide a theoretical basis .

经40%饱和度硫酸铵分级沉淀，SephadexG-200凝胶过滤等步骤，将棉铃虫血淋巴酚氧化酶纯化，纯化倍数为41.5倍，酶得率为12.7%，比活力为4030.6U／mg，酶得到了初步纯化。

The enzyme was purified by 40 % saturated ( NH4 ) 2SO4 and Sephadex G-200 gel filtration from crude enzyme with purification factor of 41.5 , and yield factor of 12.7 % , and the specific activity 4030.6U/mg . The enzyme was purified partially .

黑化是由酚氧化酶催化一系列酚类物质被氧化还原生成醌类物质，醌再经过非氧化还原反应最终生成黑色素反应过程，酚氧化酶是这一过程的关键酶（phenoloxidase，PO）。

And is catalyzed by a series of phenoloxidase phenols were generated quinones redox material , after the non-quinone oxidation-reduction reaction to generate the final response to the process of melanin pigment , phenoloxidase is the key enzyme in this process ( phenoloxidase , PO ) .

以4-己基间苯二酚（4HR）为效应物，研究了其对小菜蛾酚氧化酶单酚酶和二酚酶活力的影响，旨在为寻找新型害虫控制剂提供线索。

In order to provide theory foundation for developing the insecticide with novel modes of action , the effects of 4-hexylresorcinol ( 4HR ) on the phenoloxidase ( PO ) from Plutella xylostella , which is one of the key enzymes in the development process of insects , were studied .

湖北钉螺体内酚氧化酶及其组织学定位

Studies on phenol oxidase in Oncomelania hupensis and its histochemical localization

4-己基间苯二酚对小菜蛾酚氧化酶抑制作用研究

Inhibitory Effects of 4-Hexylresorcinol on Phenoloxidase of Diamondback Moth , Plutella xylostella

日本血吸虫酚氧化酶的组化定位研究

Studies on histochemical localization of phenol oxidase in Schistosoma japonicum

参与脱氯的关键酶系主要包括过氧化物酶和酚氧化酶。

Peroxidases and phenol oxidases are the key enzymes in this process .

儿茶酚氧化酶的催化机理及活性基团的研究

Study on the catalytic mechanism and active-site groups of CATECHOLASE

日本对虾的酚氧化酶特性研究

Study on some characteristics of phenoloxidase from Japanese prawn , Penaeus japonicus

酚氧化酶研究概况Ⅱ&基因结构及其系统演化

Advance on the study of phenoloxidase ⅱ & gene structure and its phylogenetics

棉铃虫血淋巴酚氧化酶的初步研究

Primary Studies on the Phenoloxidase from the Haemolymph of Larvae of Heliothis Armigera