转录本

绵羊CAST基因2型和4型转录本的克隆及特性分析

Cloning and characterization of CAST transcript 2 and 4 in sheep

科学家被迫将目光转向了RNA&一种DNA的直接、也更复杂的转录本。

Forced to look elsewhere , scientists turned to RNA , a direct yet more complex transcript of DNA .

单向锚定PCR富集扩增在克隆低丰度基因转录本中的应用

Cloning of Low Richness Transcripts by Using Uni lateral Anchored PCR Enrichment Amplification Method

Northern杂交分析显示，该基因mRNA转录本在两种不同胸腺基质细胞中的表达存在显著差异。

Northern blotting demonstrated differential expression of the mRNA transcript in the two thymic stromal cell lines .

RT-PCR成功验证了这个转录本的存在。

RT-PCR verified this transcript .

另外，H2可以诱导细胞内一些代表性抗氧化酶的活性与转录本。

The activities and transcripts of representative antioxidant enzymes were induced after exposure to H2 .

通过检索网上数据库资料，获得ob的转录本信息；

All reported transcripts of ob were collected through searching online database .

应用RT/PCR技术检测急性粒细胞白血病M2型AML1/ETO融合基因转录本的研究

Detection of the AML1 / ETO fusion transcript in acute myelogenous leukemia type M2 by RT / PCR

Pem基因的表达具有明显的时间和空间特异性：在鼠胚发育的第6天，即可检测到Pem转录本的表达；

Pem gene is expressed in a time and stage specific manner .

以筑巢式逆转录聚合酶链反应（NestedRTPCR）技术检测PMLRARa融合基因转录本；

PML-RARa fusion gene transcript was detected by nested-reverse transcription-polymerase chain reaction ( nested RT-PCR ) .

2例有t（15；17），PML/RARa融合基因转录本阳性，确诊为M3；

Cases had t ( 15 ; 17 ) and PML-RARa fusion gene transcript positive , diagnosed as M_3 ;

DNA测序发现FHIT基因的异常转录本是由于核普酸片段的缺失和插入突变引起的；

DNA sequencing found that the aberrant transcripts of FHIT gene were caused by deletion or insertion of nucleotide fragments .

生物技术药物以人类体细胞的基因组、转录本组和蛋白质组三个层次生物大分子为目标，基因药物的研究主要针对致病基因的DNA和基因转录本mRNA两类生物大分子。

Biotech drugs are targeting DNAs , transcripts and proteins of disease related genes in human cells , among which gene drugs focus on functional gene duplex DNAs and mRNA transcripts .

利用qRT-PCR分析检测到它的mRNA转录本在突变体叶片中下调表达。

Its mRNA transcript was found to be down-regulated expression in leaves of the mutant examined by qRT-PCR analysis .

籼稻中Wx基因的成熟转录本表达较强，而在粳稻中表达则较弱；

Wx maturing transcription expressed obviously in Indica , weakly in Japonica ;

Northern杂交证实KH基因转录本的大小为1.35kb，分布于正常人体脑组织中。

The Northern blot result showed that KH gene distributed in normal human brain tissue , whose transcript size was 1.35 kb .

结果：在所检全部胃癌组织中均未见AGO基因突变；AGO基因α和β型转录本在所有不同肿瘤细胞系中均有不同程度的表达。

Results : No mutation in regions of the AGO gene screened was revealed in the 22 primary tumor tissues and all the cell lines showed expressions of the two isoforms of the AGO transcripts .

克隆的cDNA序列比对表明，成年公鹅睾丸至少同时表达五种PRLR转录本。

Alignments of cloned cDNA fragments show that at least five PRLR transcripts expressed in testis of adult male goose .

慢性髓细胞白血病患者染色体分析及bcr／abl融合基因转录本定量的临床意义

Chromosome analysis and quantification of bcr-abl fusion gene transcripts in patients with chronic myeloid leukemia : Value of clinical application

结果：12例患者中，4例有t（8；21），AML1/ETO融合基因转录本阳性，确诊为M2；

Result : In 12 patients , 4 cases had t ( 8 ; 21 ) and AML1 / ETO fusion gene transcript positive , diagnosed as M_2 ;

CBFβ/MYH11融合基因转录本检测及其致白血病机制的研究

Detection of CBF β / MYH11 fusion transcripts and study of the mechanism of leukemogenesis of CBF β / SMHHC fusion protein

不同核背景对小麦V-CMScoxⅢ基因转录本编辑的影响

Effects of Different Nuclear Backgrounds on RNA Editing of the cox ⅲ Gene in Wheat V-CMS

HNOS外显子1f特异性转录本为SK-N-SH细胞中nNOS基因最主要的转录本。

Exon 1f-specific transcript was the major transcript of nNOS gene in SK-N-SH cells .

DLC-1基因两个转录本在胶质瘤中的表达研究

The Study on the Expression of Two DLC-1 Isoforms in Glioma

应用实时定量PCR技术检测慢性粒细胞白血病患者bcr/abl~（P190）融合基因转录本

Quantification of bcr / abl ~ ( P190 ) fusion gene transcripts by real time quantitative PCR in patients with chronic myeloid leukemia

其中Trinity组装的转录本可以评估基因组组装质量和提高基因组中编码基因区的完整性。

The transcripts that assembled by Trinity can evaluate the quality of giant panda genome assembly , and improve the completeness of coding gene region in genome .

结论AML1转录本结构的完整性对M-CSF-R的转录激活是必需的。

Conclusion An intact structure of AML1 is necessary for transactivation of M-CSF-R.

例2，应用流式细胞仪和一组单克隆抗体检测其白血病细胞的免疫表型，应用筑巢式逆转录聚合酶链反应（RTPCR）技术检测其AML1/ETO融合基因转录本。

Immunophenotyping of the blast cells was analyzed by flow cytometry with a panel of monoclonal antibodies . AML1 / ETO fusion gene was tested by nested reverse transcriptase polymerase chain reaction ( RT PCR ) .

用筑巢式逆转录酶／多聚酶链反应检测Ph1阳性白血病特异基因转录本

Reverse transcriptase Detection of BCR-ABL fusion gene in Ph1 chromo-some positive leukemia by nested

禁食时IGP-IR（用Scatchard分析）和IGF－1－rmRNA无明显的改变。再喂养后的前24小时间11-kbIGF－IrmRNA转录本明显增加（达对照组水平166%），IGF-IR数量增加3倍。

Levels of IGF-IR ( by Scatchard analysis ) and IGF-1-R mRNA transcript increased significantly during the first 24h of re feeding ( to 166 % of the control value ), and IGF-IR number rose 3-fold .