虫草素

北冬虫夏草发酵液中虫草素和腺苷含量的HPLC分析

Analysis of Cordycepin and Adenosine Contents in Fermentation Supernatant of Cordyceps militaris by HPLC

应用熔点测定、IR、以及元素分析对所得的结晶Ⅱ进行结构鉴定，结果与虫草素的资料数据一致。

The crystallize II have been characterized by TLC , HPLC , Melting Point Determine , IR , and chemical element analysis , the result is in accordance with the cordycepin spectrum data .

不同pH值提取液浸提虫草素研究

Study on Cordycepin Extraction with Different pH Value Distilling Solution

离子交换层析分离提取固体培养残基中虫草素的正交实验表明洗脱流速、料水比为主要影响因子，最优条件为：料水比1：16，离子柱pH3.5；

The orthogonal test of ion exchange chromatography showed the main influential factors were flow rate of elution and ratio of material to water .

从最优化培养基条件下进行扩大培养，对其进行菌丝体中虫草素的提取、分离、纯化，通过阴离子交换树脂得到虫草素结晶，得率为53.88mg/100g干菌丝体。

To do extraction , separation and purification by anion exchange resin and examination by HPLC to cordycepin in dry mycelia , cordycepin crystalline was gotten . The rate is 53.88mg/100g in dry mycelia .

对其进行含量测定，得虫草素结晶中虫草素的含量为88.9%。

The rate of cordycepin in cordycepin crystalline is 88.9 % .

采用柱层析分离与富集虫草素研究

Research on separation and enrichment of cordycepin by column chromatography

虫草素的紫外与红外光谱特征

Characteristics of Ultra - violet and Infrared Spectrums in Cordyceps Mili - taris

虫草素对小鼠脾淋巴细胞调节作用的研究

Study of Cordycepin on Regulation of Mouse Spleen Lymphocytes

虫草素及2′－脱氧腺苷的超导核磁共振与红外光谱鉴别

NMR and IR studies on the characterization of cordycepin and 2 ′ - deoxyadenosine

对虫草素精品和虫草素标准品红外光谱对比，确定为虫草素。

Comparing cordycepin purification with standard used infrared method , could decided it was cordycepin .

因此，许多实验室对虫草素的合成进行了研究。

As a result , the synthesis of3 ' - deoxyadenosine was investigated in many laboratories .

索氏提取结果表明水比乙醇更优于提取虫草素；

The soxhlet extraction showed that water was better than ethanol for the extraction of the cordycepin .

虫草素高产融合子在经过进一步遗传稳定测试后，可用于以获得虫草素为目的的菌丝体发酵培养。

The high yield strain could be applied in fermentation for cordycepin product after further genetic stability test .

目的：建立保健品中腺苷和虫草素含量的测定方法。

Objective : To develop a method with HPLC to simultaneously determine adenosine and cordycepin in health foods .

虫草素第一个从真菌中分离出来的核苷类抗菌素，它具有多种药理活性作用。

3 ' - deoxyadenosine is the first nucleoside antibiotic separated from epiphyte , it has many pharmacological activities .

综合虫草素含量和人工培养结果，选出16株进行遗传稳定性的测定。

Concluded from the results of cordycepin content and bottle cultivation , 16 strains were selected to test genetic stability .

虫草素和腺苷产量分析结果也表明激素可改变两种活性物质的代谢节奏。

Furthermore , both kinds of hormones also could change the metabolic rhythm of cordycepin and adenosine based on their productivity during the fermentation .

虫草素对东莨菪碱所致小鼠记忆障碍的影响白花银背藤根和茎中东莨菪内酯的含量测定

Effects of cordycepin on mice with learning and memory acquisition impairment induced by scopolamine Determination of Scopoletin in the Roots and Stalks of Herba Argyreia Seguinii

对于多糖、虫草素及类胡萝卜素的合成，白炽光显著优于黄光和红光，而对麦角甾醇和游离氨基酸则相反。

For synthesis of polysaccharide , cordycepin and carotenoids , the incandescent light is much better than yellow and red light , superior to ergosterol and free-amino acids oppositely .

北冬虫夏草是一种珍贵的药用真菌，富含虫草素和胞外多糖等功能生理活性物质，主要是通过增强机体免疫力、抗氧化、抗应激反应和抗肿瘤等以达到防治疾病的作用。

Cordyceps militaris is a precious medicinal fungus which is rich of functional and physiological active substances such as cordycepin , exopolysaccharides and so on . It achieved effects of the disease prevention and control mainly through enhanced immunity , anti-oxidation , anti-stress response and anti-tumor .

测定和制作了检测真菌激发子诱导效果的虫草菌素标准曲线，其线性范围为0～40μg／ml。

The standard curve of cordycepin have been determined and drawn under the range of 0 ~ 40 μ g / ml. It can be used as analyzing criterion for checking the affection of fungal elicitors .

现代医学研究表明虫草菌素（cordycepin）是其重要的生物活性成分，用现代生物技术手段大规模深层培养蛹虫草生产虫草菌素具有重要的应用价值。

This fungi produces an important bioactive compound , cordycepin ( 3 ' - Deoxyadenosine ) . How to enhance the cordycepin production is a bottleneck for commercial application of fungi fermentation technology .

真菌激发子诱导虫草菌素高产的条件

Inducing Conditions of Fungal Elicitor on Cordycepin Acceleration

虫草菌素的生物活性及检测方法

Biological Activity and Determination Method of Cordycepin

低能离子束修饰蛹虫草菌株高产虫草素添加前体促进蛹虫草发酵生产菌丝体和虫草菌素的研究

High-yielding cordycepin in Cordyceps militaris modified by low-energy ion beam

蛹虫草高产虫草菌素的深层培养工艺研究

Studies on Submerged Culture Technics for Cordycepin Production by Medicinal Fugus Cordyceps Militaris

人工蛹虫草固体培养基虫草素的分离纯化研究

Isolation and Purification of Cordycepin from the Solid Medium of Cordyceps militaris

蛹虫草大米培养残基中虫草素提取方法的优化研究

Study on Optimization of Cordycepin Extraction from Cordyceps Residue Medium

蛹虫草含有虫草多糖、虫草素、核苷类物质、麦角甾醇、虫草酸等多种有效成分，这些有效成分含量是蛹虫草子实体品质的重要指标。

Cordyceps militaris contains polysaccharides , cordycepin , nucleoside substances , ergosterol , cordyceps acid , and other active ingredients , these effective ingredients are important indicator of the quality of Cordyceps militaris fruiting bodies .