藁本

藁本抗炎和抗腹泻作用的实验研究

Anti-diarrhea and Anti-inflammatory Effects of Rhizoma ligustici

三个不同产地川芎与其近缘植物藁本的对比研究

The Comparative Study on between Three Different Place of Origin of Rhizoma Chuanxiong and Its Related Rhizoma Ligustici

超临界CO2流体中藁本内酯可溶性压力估算及在当归油中的溶解度测定

Soluble Pressure Estimation and Solubility Determination of Ligustilide from Angelica sinensis Oil with Supercritical CO_2 Fluid

从超临界CO2流体萃取工艺制备的浓缩颗粒（样品Ⅱ）中分离鉴定了32个成分，主要为藁本内酯、亚油酸。

32 compounds were identified from sample ⅱ, the major constituents are ligustilide and linoleic acid .

建立了TLC鉴别方法和藁本内酯HPLC含量测定方法。

TLC was established to identify medicine . Established the method of HPLC determination of ligustilide .

目的连续6年动态监测四川灌县徐渡等乡国家GAP示范基地川芎药材中的苯酞类成分，寻找其活性成分藁本内酯和川芎内酯的含量规律。

OBJECTIVE To investigate the variation of the content of phthalide compounds of Ligusticum chuanxiong Hort . in GAP base of GuanXian of Sichuan province .

方法TLC法鉴定川芎药材中的藁本内酯，并用HPLC法对药材中的藁本内酯和阿魏酸进行含量测定。

Method TLC was used to identify the content of ligustilide and HPLC was used to assay the contents of ligustilide and ferulic acid in RC .

结果表明，温度和光对藁本内酯稳定性影响较大，pH值对藁本内酯稳定性有一定影响，川芎蒸馏液浓度对藁本内酯稳定性影响不大。

Results : Temperature and light had stronger effects on the stability of ligustilide , pH value had certain effect on its stability , while the concentration of the distilled fluid had no effect on its stability .

在此基础上，研究建立了川芎中藁本内酯的HPLC含量测定方法，该方法简便、快速、重现性好，通过测定10批药材样品，藁本内酯的含量在0.52-0.74%之间。

We established the determination of ligustilide of Chuanxiong by HPLC , the method is simple , rapid , and reproducible . The content of ligustilide of Chuanxiong is between 0.52 % and 0.74 % .

辽藁本（Ligusticumjeholense）幼苗初生维管系统的发育

Studies on the primary vascular system of the seedling of Ligusticum jeholense

为考察温度、浓度、光、pH值对川芎蒸馏液中藁本内酯稳定性的影响，采用紫外分光光度法，在271nm处测定川芎水蒸汽蒸馏液的吸收度（A值）。

To investigate the effects of temperature , concentration , light and pH value on the stability of ligustilide in distilled fluid of Rhizoma Chuanxiong , ultraviolet spectrophotometric method was used to detect the absorbance ( A value ) of the distilled fluid at the wavelength of 271 nm .

结果：最优提取工艺条件为：藁本加8倍量水，浸泡4h，提取挥发油6h；

Results : The optimum preparation processes for extract was established as follow : infusion time was 4h , adding was 8 times of water as heavy as the herb , extracting volatile oil time was up to 6h .

气相色谱-质谱法测定当归挥发油中藁本内酯的含量

Determination of Ligustilide in Angelica sinensis Essential Oil by GC - MS

藁本内酯的稳定性及β-环糊精包合工艺的考察

Stability of ligustilide and its β - cyclodextrin inclusion process

川芎挥发油中藁本内酯在大鼠体内的药动学研究

Pharmacokinetics of ligustilide in Ligusticum chuanxiong essential oil in rats in vivo

中国藁本属叶柄的解剖学研究

Anatomical studies of petiole in Ligusticum from China

不同浓度多效唑对辽藁本生长·产量和挥发油含量的影响

Effects of Different Paclobutrazol Concetration on Growth Characteristics , Yield and Volatile Oil Contents of Ligusticum jeholense

本发明公开从天然产物当归中提取分离藁本内酯的方法和藁本内酯的保藏方法。

The present invention is process of extracting and separating ligustilide from angelica and method of preserving ligustilide .

其中Z-藁本内酯得率为0.025%，可作为川芎药材及提取物质量标准研究对照品。

Z-ligustilide achieved rate is 0.025 % , it can be used as reference substance for Chuanxiong quality control .

方法：采用均匀设计安排试验，对藁本颗粒大小、提取溶剂的量、提取时间等条件进行考察，得出最佳的提取条件。

Methods : By comparing the extracting method , granule size , extracting solvent and extracting time , we got the optimum extracting condition .

结论：改进后得到的最佳提取方式可以更真实地反映出藁本药材中阿魏酸的含量。

Conclusion : the ameliorated extracting method could improve the extracting efficiency greatly , and evaluate the quality of raw Rhizoma Ligustici more truly .

气相色谱内标法是一种较准确科学的方法，而紫外分光光度法是一种简单快捷又经济的方法，可供实验过程中跟踪藁本内酯含量变化。

Method of GC is more accurate ; while UV is easy , reliable , and economic , so is practical for catching variation of ligustilide content in experimentations .

当串珠藁本密度由0增至60万株／666.7m2时，小麦产量损失由0增至37.7%。

When the density of Ligusticum moniliforme was increased from 0 to 600 thousand plants per 666.7 m 2 , the wheat yield was decreased from 0 to 37.7 percent .

结果：当归补血汤汤剂中挥发油的主要成分为反式藁本内酯、正丁烯基苯酞、正丁基苯酞、顺式藁本内酯等。

The temperature rate was 3 ℃· min - 1 . RESULTS : The main components of volatile oil of Danggui Buxue Decoction were composed of trans-ligustilide , butylidene phthalide , n-butylphthalide , and cis-ligustilide .

清水镇当归挥发油的主要成分为丁烯基苯酞、Z-藁本内酯、异匙叶桉油烯醇、6-丁基-1,4环庚二烯，其相对百分含量为66.8%。

The main component of volatile oil in Qingshui town : Benzene phthalate butene , Z-ligustilide , different key enol Eucalyptus oil , 6 - diene butyl 1,4-cycloheptane , and the relative percentage of 66.8 % .

当归油的提取是以乙醇水溶液为溶剂，先浸渍当归粉，再回流，然后蒸馏得当归油，经红外光谱法鉴定，含有藁本内酯这一主要有效成分。

Ethanol and water was used as solvent to infuse angelica powder , then angelica oil was obtained by refluxing and distilling extractant . Fourier transform infrared spectroscopy analysis shows that angelica oil contains the efficient component ligustilide .

TNF-α对照组表现出凋亡形态学表现的细胞数量高于比其他组，藁本内酯治疗组的凋亡细胞数量较TNF-α对照组少，并呈现一种量效关系。

The number of cells exhibiting typical apoptotic morphology in the TNF-a-control group was higher than the number of apoptotic cells in the other groups . In contrast , the ligustilide-treated groups presented reduced numbers of apoptotic cells in a dose-dependent manner .