苏木素染色

陈旧性病理组织苏木素染色方法

Hematoxylin staining of old pathologic tissue

方法：改进显示缺氧心肌的碱性复红法，即延长苏木素染色时间并增加盐酸酒精分化的步骤。

Methods : The basic fuchsin method used to show the anoxic cardiac muscle was modified by prolonging the hematoxylin staining time and adding a step of hydrochloric acid alcohol differentiation .

用改良的Mayer氏苏木素染色法显示胃颈粘液细胞

Displaying Gastric Mucous Neck Cells by a Modified Method of Mayer 's Hematoxylin Stain

苏木素染色（+）的CIN和宫颈癌病例与慢性宫颈炎、息肉等良性宫颈病变病例统计相比，有显著性差异（P<0.01）。

The cases of CIN and cervical cancer with positive cervical hematoxylin coloration test were significantly larger than those of chronic cervicitis , cervical polyp .

苏木素染色后，细胞核呈淡紫色，复苏细胞存活率在90%以上，可用于试验。

After haematoxylin staining , cells nuclei were light purple , and living cells ratio was up to90 % from recovery by Trypan blue staining .

应用伊红-苏木素染色法（HE），可观察到纯化的细胞形态多样，呈多角形或卵圆形，细胞界限清楚，胞浆透明，核圆或呈小泡状。

Application Yihong-hematoxylin staining ( HE ) can be observed in the purified cells , which have diverse polygonal , oval , clear cell boundaries . The cells have transparent cytoplasm , round nuclear or a small bubble .

采用TUNEL结合苏木素-伊红染色、神经元Nissl染色等方法检测脑细胞凋亡；

TUNEL , Haematoxylin and Eosin , and Nissl staining were used to detect neuronal apoptosis .

方法8只犬Marshall韧带大体解剖观察后连续组织切片，分别采用伊红-苏木精（HE）染色、磷钨酸苏木素（PTAH）染色，在光学显微镜下观察其组成和组织特征。

Method The ligaments of Marshall of 8 canines were studied by serial sections under the light microscope .

4HE染色石蜡切片经过二甲苯脱蜡，梯度酒精水化，苏木素-伊红染色，再梯度酒精及二甲苯脱水，封片。

HE staining paraffin sections xylene dewaxing , gradient ethanol hydration , hematoxylin-eosin stain , then gradient alcohol and xylene dehydration , mount .

苏木素-伊红染色，光镜下随机计数分析预缺血后海马CA1区死亡神经元数量变化。

Neuronal death in the CA1 region was observed by hematoxylin-eosin staining , and number of live neurons was assessed by cell counting under a light microscope .

方法CCl4所致2、4、6、8周肝纤维化模型及对照组大鼠肝苏木素-伊红染色、免疫组织化学反应，观察肝组织病理变化和IL-1β及IL-1βR的表达。

The liver tissues of rats in all groups ( including the control group ) were stained by hematoxylin-eosin ( HE ) and the pathological changes and the expressions of IL-1_ β and IL-1_ β R in rat liver tissues were ( observed ) with immunohistochemical response .

苏木素与伊红染色观察肝脏活组织检查。

Furthermore , liver biopsies were visualized by hematoxylin and eosin staining .

用苏木素-伊红染色观察缺血后神经元坏死情况；

Neural necrosis was observed with hematoxylin and eosin ( HE ) staining .

结论：脑脊液墨汁或苏木素－伊红染色检查有助于鉴别。

Conclusion : The CSF India-ink / hematoxylin-eosin staining is critical to diagnose .

各时相点取材，苏木素-伊红染色观察病理改变，并进行脑含水量测定；

Pathological changes were observed with hematoxylin-eosin staining , and content of water in brain was detected ;

苏木素-伊红染色观察心肌细胞形态，鉴定细胞衰老的生物学标志β-半乳糖苷酶观察细胞衰老情况。

Formation of cardiac myocytes was observed with Hematoxylin and eosin staining . Cell aging was observed with β - galactosidase , which was the biological marker of senescence .

方法：采用下列三种方法去除HE染色切片中的苏木素沉渣并比较其效果：（1）陈旧苏木素染色液加热至40℃；

Methods : Three methods as follow were employed to eliminate the hematoxylin sediments in HE sections : 1.Over-used hematoxylin solution was heated to 40 ℃;