胭脂碱

把带有甜蛋白基因和胭脂碱合成酶标记基因的Ti质粒导入马铃薯，获得大量转化植株。

A number of transgenic plants were obtained using the plasmid containing a sweet protein NPT ⅱ and nopaline synthase genes .

应用根癌农杆菌胭脂碱合成酶基因（nos）终止子和大肠杆菌K12菌株新霉素磷酸转移酶Ⅱ（nptⅡ）基因的二重PCR对样品进行转基因成分检测，结果均为阴性。

The duplex PCR detection results of Agrobacterium tumefaciens nopaline synthase ( nos ) terminator and Escherichia coli strain K12 neomycin phosphotransferase II ( npt ⅱ) gene in these samples were all negative .

A（84）的敏感性与胭脂碱Ti质粒及宿主的关系

A_ ( 84 ) sensitivity determined by both nopaline type Ti plasmid and its host cell

GUS活性检测和胭脂碱分析表明，外源基因在转化细胞内得到稳定表达。

Enzyme activity of GUS and NOS assay demonstrated that foreign genes expressed in the transformed plants and calli .

花生畸胎瘤冠瘿瘤的诱导及胭脂碱合成酶基因的转移

Teratoma Induction and the Nopaline Synthase Gene Transfer in Peanut

大豆属致瘤农杆菌致瘤后胭脂碱的测定

Determination on nopaline dehydrogenase from tumor callus of soybean plants caused by

取转化苗叶片作胭脂碱合成酶活性检测电泳后呈阳性；

The nopaline synthetic enzyme activity of the preliminary transformation was detected by electrophoresis .

其中以开花初期的植株，利用胭脂碱型农杆菌介导（如C58），真空渗入5分钟的处理方法转化效率最高。

It was concluded that using the nopaline type of Agrobacterium ( e.g.C58 ), 5min vacuum infiltration , and using the plants with just several blooming flowers would give the best results .