胆固醇氧化酶

调节诱导阶段温度和pH提高活性胆固醇氧化酶产率，建立双程培养策略。

Increased soluble cholesterol oxidase production yield by change temperature and pH in induction phase , and established the Two-phase culture strategy .

重组E.coli胆固醇氧化酶的分离纯化及其酶学性质研究

Purification and Partial Characterization of Cholesterol Oxidase from Recombinant E.coli

提高重组E.coli产胆固醇氧化酶水平的研究

Research on Improving Recombinant E.coli to Express Cholesterol Oxidase

短杆菌BrevibacteriumSP.胞外胆固醇氧化酶对蛋黄胆固醇的生物转化（英文）

Bioconversion of Yolk Cholesterol by Extracellular Cholesterol Oxidase from Brevibacterium sp .

醋酸纤维素（CA）作为一种优异的生物传感用薄膜制备材料，是胆固醇氧化酶（COD）优良的固体载体。

As a excellent material for preparation of biomembrane , cellulose acetate ( CA ) is a fine carrier for the immobilization of cholesterol oxidase ( COD ) .

在研究了其浊点、浓度、pH值、分离时间和分离温度等影响因素后，得到了分离胆固醇氧化酶的优化条件。

Using this method for separation of cholesterol oxidase , the optimized separation condition were determined on the basis of the studies of the Triton X-114 cloud point , surfactant concentration , pH , mixture time and temperature .

Rhodococcusequi4-2胆固醇氧化酶的发酵、纯化及初步应用

Production , Purification and Primary Application of the Cholesterol Oxidase from Rhodococcus Equi 4-2

3.7L发酵罐中研究了工程菌的培养策略，进一步提高活性胆固醇氧化酶产率。

Studied the culture strategies of engineering bacteria in 7L ' fermenter to further improve the yield of cholesterol oxidase activity .

纯化的胆固醇氧化酶是分子量为58kDa的单体酶，在280nm处有最大吸收峰。

The purified enzyme was a monomer with a molecular weight of 58 kDa , and exhibited maximum absorption at 280 nm .

从乳化体系中分离纯化短杆菌产胆固醇氧化酶

Purification of a Cholesterol Oxidase from Brevibacterium sp. in Emulsified System

膜胆固醇氧化酶的萃取及特性研究

Research on Extraction and Physical Properties of Membrane-bound Cholesterol Oxidase

马红球菌4-2胆固醇氧化酶的急性毒理学研究

Acute Toxicology Research on the Cholesterol Oxidase Produced by Rhodococcus equi 4-2

产胆固醇氧化酶重组大肠杆菌的发酵培养基和诱导条件的优化

Optimization of Culture Conditions for Production of Cholesterol Oxidase Using Recombinant E.coli

聚吡咯电化学固定化胆固醇氧化酶电极的电流响应及应用

Application and Current Response of Electrochemical and Immobile Polypyrrole Cholesterol Oxidase Electrode

胆固醇氧化酶法脱除食品中胆固醇的研究进展

The research of the degradation of cholesterol by cholesterol oxidase from food

采用胆固醇氧化酶作脱除猪油中胆固醇的试验。

Removing cholesterol experiments by cholesterol oxidase is completed .

用胆固醇氧化酶脱除猪油中的胆固醇

Removing Cholesterol Contained in Lard by Utilizing Cholesterol Oxidase

构建胆固醇氧化酶的短杆菌基因文库

Construction of Brevibacterium genomic library for cholesterol oxidase production

高活性胆固醇氧化酶产生菌出发菌株的筛选

Screening of strains Producing Cholesterol Oxidase with High Activity

氮源单因素试验证明，胰蛋白胨是产胆固醇氧化酶的最佳氮源。

Best nitrogen source was proved to be tryptone .

胆固醇氧化酶分离纯化及应用的研究进展

Research Progress of Purification and Application for Cholesterol Oxidase

胆固醇氧化酶降解效果的研究

Research on the Decomposing Effects of Cholesterol Oxidase

双水相非离子表面活性剂萃取法分离胆固醇氧化酶

Separation of Cholesterol Oxidase by a Nonionic Surfactant - based Aqueous Two-phase Extraction System

胆固醇氧化酶高产菌株的诱变选育及产酶条件优化

Screening of high-yield strains of cholesterol oxidase by ultraviolet mutation and optimization of fermentation conditions

其他筛选菌株的胆固醇氧化酶的鉴定及制备工作正在进行中。

Preparation and characterization of cholesterol oxidases from the other selected strains are under way .

胆固醇氧化酶分批发酵动力学模型的建立

Kinetic model of cholesterol oxidase batch fermentation

胆固醇氧化酶发酵研究

Studies on the Cholesterol Oxidase Fermentation

我们也对聚吡咯&胆固醇氧化酶电极进行了初步的研究和探讨。

In this dissertation , the conditions of the preparation of polypyrrole-cholesterol oxidase electrode were also discussed .

采用乳糖诱导胆固醇氧化酶表达，提高了胆固醇氧化酶可溶表达的比例，并优化了乳糖诱导胆固醇氧化酶表达的条件。

Increased the proportion of soluble cholesterol oxidase by lactose induction and optimized the conditions of cholesterol oxidase expression induced by lactose .

胆固醇氧化酶等的固定化酶制备及在微生物传感器中的应用

The Prepartion of Immobilized Enzyme ( COD , CEH , POD ) for Cliniced Diagnosis and Its Use in Biosensor of Microbic Bacteria