磷酸转移酶

在上述浓度范围内，带潮霉素磷酸转移酶基因（hpt）的Bt转基因水稻（克螟稻）幼苗均未出现中毒症状。

All of the transgenic rice with hygromycin phosphotransferase gene ( hpt ) was resistant to hygromycin B , none appeared any toxic symptoms over the concentrations mentioned above .

潮霉素磷酸转移酶基因hpt在68.4%的转基因植株中符合孟德尔遗传模式；

For segregation patterns of hygromycin phosphotransferase gene ( hpt ) , 68.4 % of the transgenic rice plants were conformed to a Mendelian ratio .

抗性转化子的Southern分析表明，潮霉素磷酸转移酶基因已整合到受体黑曲霉的基因组DNA中。

Southern blot analysis showed the hph gene has been integrated into genomic DNA of A. niger transformant .

另一个C端则含有磷酸转移酶的相同序列，估计与催化功能相关。

While the C-terminal of the choline kinase contained a phosphotransferase consensus sequence , which was presumed to be involved in catalytic function .

无HindⅢ位点新霉素磷酸转移酶标记基因的建立

Construction of a neomycin phosphotransferase gene without hind ⅲ site

潮霉素磷酸转移酶（HPT）是转基因作物中重要的标记蛋白，其活性的测定对含有该标记基因的转基因作物的植株构建、基因沉默、标记基因去除及安全性分析等具有重要的作用。

Hygromycin B phosphotransferase ( HPT ) is a widely used selectable marker protein of transgenic plant .

葡萄糖主要通过磷酸转移酶系统（phosphotransferasesystem，PTS）被宿主细胞磷酸化和内质化，然后才能进一步被菌体分解代谢，参与并维持菌体的生理活动。

The glucose phosphorylation and endoplasmic reticulum in host cells is mainly directed by the phosphotransferase system ( PTS ) . Then it can be further metabolized by bacteria to participate and to maintain its physiological activities .

应用根癌农杆菌胭脂碱合成酶基因（nos）终止子和大肠杆菌K12菌株新霉素磷酸转移酶Ⅱ（nptⅡ）基因的二重PCR对样品进行转基因成分检测，结果均为阴性。

The duplex PCR detection results of Agrobacterium tumefaciens nopaline synthase ( nos ) terminator and Escherichia coli strain K12 neomycin phosphotransferase II ( npt ⅱ) gene in these samples were all negative .

目的：制备抗潮霉素B磷酸转移酶（HPT）的单克隆抗体（McAb），建立一种快速检测转基因作物中该选择标记基因HPT编码蛋白的方法。

Objective : To produce monoclonal antibodies ( McAb ) against hygromycin B phosphotransferase ( HPT ) and to establish a rapid method for the measurement of HPT antigen in the genetically modified crops ( GMC ) .

将绿脓杆菌外毒素、2A序列、IL2受体穿膜区以及新霉素磷酸转移酶的基因依次融合构建新型报告基因&peo，该报告基因能够特异性地甄别具有信号肽编码序列的基因。

A new reporter gene named peo was obtained by in turn fusing Exotoxin A of Pseudomonas aeruginosa , 2 A sequence , transmembrane region of IL-2 receptor and neomycin phosphotransferase genes , Peo is able to report genes encoding secretory proteins specifically .

转基因作物标记蛋白潮霉素磷酸转移酶活性的测定方法

Methods of hygromycin B phosphotransferase activity assay in transgenic plant

氨基糖苷磷酸转移酶丙氨酸在转氨作用中似乎是更好的氨基酸。

Alanine appears to be the preferred amino acid in the transamination .

豚鼠心脏CDP-乙醇胺磷酸转移酶的动力学研究

The kinetic study of CDP-ethanolamine phosphotransferase in guinea pig heart

潮霉素磷酸转移酶的致敏性评价研究

Study on Allergenicity Assessment of hygromycin B phosphotransferase Protein

胆碱磷酸转移酶基因转化大豆的初步研究

The research on transfering CPT gene into soybean

基因枪转化将潮霉素磷酸转移酶基因导入粳稻品种77170，获得可育的转基因植株，研究外源基因遗传的稳定性。

Japonica rice cv77170 was transformed with pBI222 carrying hygromycin phosphotransferase gene by using biolistic bombardment and fertile transgenic rice plants were obtained .

而骨磷含量、骨和血清碱性磷酸转移酶与青年对照组比较有所升高，差异有统计学意义（P<0.01）；

However , the content of bone phosphorus , the activity of bone and serum ALP had statistical significance and increased ( P < 0.01 ) .

谷胱甘肽-S-转移酶P1启动子调控的胞嘧啶脱氨酶和尿嘧啶磷酸核糖转移酶融合基因对卵巢癌顺铂耐药细胞的体外杀伤作用

Killing effect of adenovirus mediated fusion gene cytosine and deaminase uracil phosphoribosyl transferase directed by glutathione S-transferase P1 promoter on cisplatin-resistant ovarian cancer cells in vitro

应用RT-PCR法检测各实验组腺苷激酶（AK）和次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）基因转录产物含量的变化。

RT-PCR was used to examine the gene transcripts concentrations of adenosine kinase ( AK ) and hypoxanthine-guanine phosphoribosyltransferase ( HGPRT ) in each group .

Fcy：：Fur基因由Fcy和Fur两个基因融合而成，其中，Fcy编码酵母菌胞嘧啶脱氨酶，Fur编码尿嘧啶磷酸核糖转移酶。

Fcy : : Fur gene was fused by two genes , Fcy and Fur . Gene Fcy encodes yeast cytosine deaminase and gene Fur encodes uracil phosphoribosyltransferase .

mdrl启动子调控胞嘧啶脱氨酶-尿嘧啶磷酸核糖转移酶融合基因对卵巢癌裸鼠耐药移植瘤的抑制作用

Anticancer effects of cytosine deaminase and uracil phosphoribosyl transferase fusion gene driven by mdrl promoter on drug-resistant ovarian cancer

本文采用改良的次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）试验，观察人皮角朊细胞铅离子体外染毒的遗传毒性。

An improved HGPRT assay system was used to investigate the genotoxicity of lead in human cutaneous keratinocytes exposed to the metal ion in vitro .

次黄嘌呤鸟嘌呤磷酸核糖转移酶（Hprt）参与嘌呤核苷酸的补救合成。恶性疟原虫次黄嘌呤鸟嘌呤黄嘌呤磷酸核糖转移酶细胞免疫保护作用的研究

The enzyme hypoxanthine-guanine phosphoribosyltransferase ( HPRT ) plays an important role in the purine salvage pathways . Evaluation of Cell-mediated Protective Immunity to Hypoxanthine Guanine Xanthine Phosphoribosyl Transferase ( HGXPRT ) of Plasmodium Falciparum

目的探讨联合应用尿嘧啶磷酸核糖转移酶（UPRT）基因对胞嘧啶脱胺酶（CD）/氟胞嘧啶（5-FC）前药转换酶系统抗瘤作用的增强效应。

Objective To investigate the enhanced antitumor effect of cytosine deaminase / 5 fluorocytosine enzyme prodrug system ( CD / 5 FC ) combined with uracil phosphoribosyl transferase ( UPRT ) gene therapy on gastric cancer xenografts in 615 mice .

次黄嘌呤鸟嘌呤磷酸核糖转移酶（HPRT）基因在单基因突变的研究中是一个经典的基因位点。

Hypoxanthine guanine Phosphoribosyl Transferase ( HPRT ) is a classical gene locus in the research of single gene mutation . The principle of HPRT locus mutation detection is summarized and some methods of detection are presented .

弓形虫次黄嘌呤-黄嘌呤-鸟嘌呤磷酸核糖转移酶基因的克隆与原核表达

Cloning and Expression of Hypoxanthine-xanthine-guanine Phosphoribosyltransferase Gene of Toxoplasma Gondii

目的鉴定日本血吸虫中存在的腺嘌呤磷酸核糖转移酶，分析其蛋白结构特征。

Objective To identity adenine phosphoribosyltransferases in Schistoma japonicum and analyze their structural features .

次黄嘌呤-鸟嘌呤磷酸核糖转移酶缺陷型杂交瘤细胞的快速筛选方法

A Rapid Method to Select HGPRT Lacking Hybridoma

尼克酰胺磷酸核糖转移酶功能研究进展

Progress in functional research of nicotinamide phosphoribosyltransferase

微生物谷氨酰胺转胺酶发酵培养基的优化尼克酰胺磷酸核糖转移酶功能研究进展

Optimization of Fermentation Medium of Microbial Transglutaminase ; Progress in functional research of nicotinamide phosphoribosyltransferase