熔解曲线

并作重现性和熔解曲线分析。

The reproducibility and melting curves of the genes were also analyzed .

实验条件对荧光共振能量转移的探针熔解曲线影响

Influence of experimental conditions on probe melting curves based on fluorescence resonance energy transfer

PCR反应完毕作熔解曲线分析。

After PCR melting curve analysis was done .

熔解曲线分析显示产物为单特异峰，Tm为86.5±0℃。

The melting curve showed a single peak with a 86.5 ± 0 ℃ Tm .

时间（英文）实时聚合酶链反应熔解曲线分析检测APC基因突变

Detection of APC mutation by real - time polymerase chain reaction and melting curve analysis

用熔解曲线法分析插入/缺失多态性和Y染色体SNPs多态性

Analysis of insertion / deletion polymorphisms and Y-SNPs with melting curve genotyping in forensic science

成功建立了一种能够区分猪瘟强毒感染与疫苗弱毒的荧光定量RT-PCR结合熔解曲线分析方法。

In order to development a fluorescence quantitative RT-PCR combined melting curve analysis method that can differentiation of Wild-type and C-Strain Vaccine Viruses of Classical Swine Fever Virus .

结果：30份标本的扩增产物经熔解曲线分析及DNA琼脂糖凝胶电泳，证实均为COX-2和GAPDH。

RESULTS : The specificity of products was proved to be COX-2 and GAPDH by the analysis of the melting curve of the amplified products and agarose gel electrophoresis .

高分辨率熔解曲线法（HRM）是一项近年来发展起来用于检测核酸序列变异的技术，它可以最大程度的满足上述临床需求。

High resolution melting ( HRM ) is an emerging technique for detection of nucleic acid sequence variation that has enormous potential to meet these clinical demands .

利用高分辨率熔解曲线分析法检测结直肠癌中KRAS基因突变

Detecting KRAS mutations in colorectal carcinoma patients with high resolution melting curve analysis

PCR熔解曲线法测定YMDD变异株与ALT和AST及HBV-DNA荧光定量联合检测对慢性乙型肝炎患者的诊断，治疗有极其重要的价值。

YMDD mutation of PCR melting curve and ALT and AST and HBV-DNA fluorimetry quantitative assay play an important role in the diagnosis and treatment of chronic hepatitis B .

比较了三种基于SSR分子标记的指纹图谱构建方法，即琼脂糖凝胶电泳检测、TP-M13-SSR毛细管电泳荧光检测和SSR高分辨率熔解曲线分析检测的优劣，提出高效农作物品种鉴定和指纹图谱构建的策略。

The advantages and disadvantages of agarose gel electrophoresis , TP-M13-SSR capillary fluorescence detection and SSR high resolution melting curve analysis were compared . A suitable strategy for crop cultivar identification and fingerprinting was proposed .

研究结果表明，SSR高分辨率熔解曲线分析检测方法适用于水稻品种高效鉴定，具有高通量、高灵敏度、自动化、快速、直观等特点，满足大小不同规模样品快速、准确鉴别的要求。

The results showed that the method based on combination of SSR molecular markers with high resolution melting curve analysis ( SSR-HRM ) was suitable for rice cultivar identification and had the characteristics of high throughput , high sensitivity , automatic , fast , and intuitive . 7 .

方法利用荧光共振能量转移的原理，设计检测人葡萄糖6磷酸脱氢酶（G6PD）基因的荧光杂交探针，在熔解曲线模式下，设定不同模板量、Mg2+浓度，观察熔解曲线峰面积及其Tm值。

Methods A pair of G6PD specific fluorescein labeled oligonucleotide hybridization probe were designed according to the principles of fluorescence resonance energy transfer . The melting curve peak area and melting temperature were observed at different concentrations of template and Mg 2 + for the mode of melting curves .

微孔玻璃中~4He熔解曲线上的超流相变

The superfluid transition on the melting curve of ~ 4he in vycor

高分辨率熔解曲线技术及其在分子诊断中的应用进展

Application of high resolution melting analysis in molecular diagnosis

高分辨率熔解曲线分析&分子诊断的新技术

High Resolution Melting-New Method for Molecular Diagnosis

当片段一定的情况下，单个碱基的差异能反映在解链温度上，表现在熔解曲线的差异。

A single base change can be reflected in the melting temperature difference , the performance difference in the melting curve .

经熔解曲线分析，其扩增产物中未检出非特异性产物和引物二聚体；

A melting curve analysis shows that , the quantity of unspecific PCR products have witch fewer primer dimers than other amplification products .

结果熔解曲线分析获得的150个基因型分析结果与测序结果100%一致。

Melting curve analyses were verified by sequencing . Results Genotyping of 10 SNPs in 15 samples with melting curve analyses was 100 % correspondence with the results of sequencing .

高分辨熔解曲线分析技术可以直接在基因水平将念珠菌鉴别，耗时短、结果准确，使临床特异、快速鉴定常见致病性念珠菌成为可能。

High-resolution melting curve technology can directly identify a variety of Candida at the molecular level . It was time-saving , accurate , and clinically specific , making rapid identification of pathogenic Candida possible . 4 .

研究了高温高压下三相碳（石墨、金刚石、液相碳）的状态方程，包括高压下石墨到金刚石的固－固相变以及高温下石墨和金刚石的熔解曲线。

Equation of state ( EOS ) of three phase carbon ( graphite , diamond , and liquid ), including solid solid phase transition from graphite to diamond under high pressure and melting line of graphite and diamond under high temperature has been studied .