溶菌

目的：通过噬菌体特性观察，为了解噬菌体吸附特异性及溶菌机理的分子生物学机制奠定物质基础。

AIM : To understand the specific adsorption and bacteriolysis mechanism by observing the biological characteristics of the bacteriophage .

采用1.0%的聚甘露糖醛酸多糖作为免疫药物，对中国对虾进行注射刺激后，发现血清中的溶菌和溶血活性有显著提高；

After Penaeus chinensis were stimulated by injection with 1 . 0 % polymannuronic acids , the activities of bacteriolysis and hemolysis in the serum increased , meat and the activities of ACP and ALP in the hepatopancreas enhanced obviously .

溶菌活力随pH值的下降而降低，但核苷酸组溶菌活力明显高于空白饵料组（P＜0.05）。

Bacteriolytic activity decreased along with pH.

枯草芽孢杆菌（Bacillussubtilis）S9对植物病原真菌的溶菌作用

Cell-lytic effect of Bacillus subtilis on plant fungal pathogens

C2组溶菌活性显著高于其他组，其他各组间差异不显著。

Bacteriolytic activity in the C2 group was higher than in the other groups .

本文测定了大黄鱼表皮、鳃、肠粘液的溶菌活性、IgM含量及蛋白质组成等生理生化特性，并研究了粘液对致病性溶藻弧菌的抗菌活性及溶藻弧菌对粘液的营养利用情况。

The physicochemical characteristics of skin , gill and intestinal mucus of large yellow croakers are studied .

构建的表达载体pPROHTa-rcLYZ含有犬溶菌基因完整的ORF；

And it contained the entire ORF of canine lysozyme gene ;

通过CMSephadexC50离子交换层析，对RX17溶菌酶粗制品进行了初步分离，得到了3个有溶菌活力的组分。

Through CM-Sephadex C-50 ion-exchange chromatography , three proteins with lytic activity were separated from the crude extract of RX-17 .

在溶菌后，胞壁的LPS被释放入血液循环系统，通过刺激单核细胞、巨噬细胞、中性粒细胞、血小板及内皮细胞而激活免疫系统。

After lysis of bacterial cells LPS is released into the circulation and it is able to activate the immunological system by stimulation of monocytes , macrophages , neutrophils , blood platelets and endothelial cells .

注射7d后观测外周血和脾脏中淋巴细胞ANAE阳性率、血清旁路途径溶血活性、血清溶菌活性。

After 7 days , we measured α - naphthyl acetate esterase ( ANAE ) activity in peripheral blood and spleen , hemolytic activity and bacteriolytic activity .

对照组和处理Ⅰ组血清溶菌活力和补体C3含量显著下降，而处理Ⅱ～Ⅳ组无显著变化；

Serum bacteriolytic activity and the level of complement C3 in the stressed C and ⅰ significantly declined , while there were no significant changes in ⅱ - ⅳ compared with non-stress groups .

对虾的血细胞数、溶菌（LSZ）活力、抗菌活力、超氧化物歧化酶（SOD）和酚氧化酶（PO）均显著高于对照池（P<0.05）；

The number of blood cell , the activity of phenoloxidase , superoxide dismutase ( SOD ), antibacterial enzyme and lysozyme were significantly higher ( P < 0.05 ) than the control pond .

与对照组相比，C0组外周血T淋巴细胞ANAE阳性率和血清旁路途径溶血活性均有显著提高，脾脏T淋巴细胞和血清溶菌活性没有显著变化。

C0 group showed higher ANAE positivity in blood and hemolytic activity than the control , but showed no significant difference in bacteriolytic and ANAE activity in spleen .

取幼鳖血液，用镜检法测定血细胞的吞噬率，透射比浊法测定血清溶菌活力、杀菌活力以及补体C3和C4含量。

The blood in turtles were collected , and the percentage phagocytic rate of blood cell was analyzed by counting under microscope ; serum bacteriolytic activity , bactericidal activity , levels of complement C3 and C4 were analyzed by transmission colorimetric method .

5种微生态制剂对溶菌活力的促进作用尤为显著，第40d，添加了N3、E5、N3+E5和枯草芽孢杆菌组则分别比对照组提高了195.2%、222.7%、153.0%和113.6%；

The lysozyme activity fed with dietary N_3 , E_5 , N_3 + E_5 , Bacillus subtilis were 195.2 % , 222.7 % , 153.0 % , 113.6 % higher respectively than those of the control group . Moreover , these probiotics also had some effects on the SOD activity .

采用玻片凝集试验和补体溶菌试验检测pCI-PIB免疫小鼠血清的抗菌活性。

By using slide agglutination test and complement bacteriolytic test , the serum anti-bacterial activity of the pCI-PIB immunized mice was determined .

经酸应激处理，血细胞吞噬率、血清溶菌活力、杀菌活力和补体C3含量，处理Ⅰ～Ⅳ组的均显著高于对照组，处理Ⅳ组显著高于其他4组；

Among of the acid stressed juvenile turtles , the phagocytic rate of blood cell , serum bacteriolytic activity , bactericidal activity and level of complement C3 in ⅰ - ⅳ were significantly higher than that in C , and ⅳ was significantly higher than the other four groups .

人工虫草菌菌丝体的破壁溶菌研究

Studies on the Breakage and Lysis of Cultured Cordyceps sinensis Mycelia

溶菌活力提高97.6%和41.5%；

The bacteriolytic activity was enhanced by 97.6 % and 41.5 % ;

同时，对76株结核分支杆菌临床分离株按碱性溶菌法抽提分离并鉴定是否含有质粒。

Plasmids were extracted from 76 clinical isolates of M.tuberculosis with alkaline bacteriolytic methods .

溶菌活性对离子强度的变化高度敏感。

The lytic activity was very sensitive to the variety of the ionic strength .

应用自发突变的原理成功地从溶菌液中筛选到抗噬菌体的工程菌株；

An anti-bacteriophage engineering Escherichia coli was directly selected from the ferment lysate by spontaneous mutation .

草鱼的血清、体表和肠粘液中溶菌物质活性及其特性

Activities and characterization of bacteriolytic substances in serum , skin and intestine mucus of grass carp

木霉菌的作用机制多种多样，包括产生抗生素、重寄生作用、溶菌作用、竞争作用等。

The antifungal mechanism of Trichoderma is varied , including antibiotic , dissolve hypha , parasitism and competition .

与对照组相比，实验组螯虾肌肉的溶菌和抗菌、超氧化物歧化酶活力没有明显的变化。

SOD , bacteriolysis and antibacterial activities in the muscle between test and control groups have no evident differences .

纸片法所测溶菌圈到3天，直径可达16.3mm。

The diameter of lysed zone produced by the antibiotic B-3543 ( paper method ) was 16.3 mm up to 3 days .

基于一些重要的生物学意义，提出一类更常见的具有溶菌性免疫反应的时滞病毒感染模型。

A class of more general delayed viral infection model with lytic immune response is proposed based on some important biological meanings .

三种组织中的溶菌物质均显示出对热的相对稳定性，分别在50℃或60℃处理10分钟后，仍能保持一定的活性。

The bacteriolytic substances activities were relatively stable to heat , and remained active when heated at 50 ℃ and 60 ℃ for 10 min.

目的：β-防御素-2在宿主防御病原微生物发挥着着重要作用，其不仅具有直接溶菌作用，同时也具有免疫调节的功能。

Objective : Beta-defensin-2 ( BD-2 ) plays an important role in host defense against pathogenic microbe challenge by its direct antimicrobial activity and immunomodulatory functions .

试验组与对照组中对虾的溶菌活力、总超氧化物歧化酶活力差异不明显（P>005）。

Both bacteriolytic activities and the superoxide dismutase activities in serum of shrimp from test and control groups had no significant difference ( P > 0 05 ) .