溴乙锭
- 网络Ethidium bromide;ethidium bromide, EB
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方法:通过DNA结合溴乙锭后的荧光损失和DNA琼脂糖凝胶电泳的迁移率来测定DNA的损伤程度。
Methods : DNA damage was measured by the loss of fluorescence after DNA binding to ethidium bromide and by the increased DNA mobility in agarose gel electrophoresis .
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方法通过吖啶橙/溴乙锭双荧光染色法检测细胞凋亡,并通过MTT法检测细胞生长抑制;
METHODS By utilizing acridine orange / ethidium bromide double fluorescent dye staining to detect the apoptosis and by using MTT to detect the growth inhibition ;
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溴乙锭荧光法测定环境污染物的DNA交联作用
Detecting the Formation of Interstrand DNA Crosslinks of Environmental Pollutants by Ethidium Bromide Assay
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双大环多胺Zn(Ⅱ)配合物及其与DNA作用的溴乙锭荧光探针
Ethidium Bromide Fluorescence Probe on the Interaction between Dinuclear Macrocyclic Polyamine Zn (ⅱ) Complex and DNA
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为了考查新聚合物和质粒形成的复合物的自聚过程,琼脂糖凝胶电泳,溴乙锭荧光发射,多角度激光散射和透射电镜从不同的方面显示了聚合物和质粒DNA之间的作用和质粒的缩合。
Agarose gel electrophoresis , ethidium bromide displacement assay , multi-angle laser light scattering , transmission electron microscopy were used to examine self-assembling between polymer and plasmid from various aspects .
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)为材料,比较了随机扩增多态DNA(RAPD)和DNA扩增指纹(DAF)方法。用RAPD的琼脂糖胶电泳和溴乙锭染色,RAPD和DAF谱一般不足10条带。
In case of using agarose gel electrophoresis with ethidium bromide ( EB ) staining which was traditionally used in RAPD , both methods generally showed simple profiles with less than 10 bands .
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溴乙锭/吖啶橙(AO/EB)荧光染色后细胞的凋亡情况;
Moreover , for qualitative determination of EC apoptosis , acridine orange ( AO ) / ethidium bromide ( EB ) fluorescence staining was used and the apoptotic rate was computed .
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与溴乙锭染色端粒重复扩增技术相比,银染端粒重复扩增技术可见到更多更清晰的带形。
The silver-staining telomere repeat amplification protocol could display more and clearer telomerase band compared with the ethidium-staining telomere repeat protocol .
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反应结束后,取反应液10ul以含溴乙锭的2%琼脂糖电泳30分钟(5V/cm),紫外分析仪上观察若在501bp处出现橙黄色条带,则为阳性标本。
After the reaction , the reaction solution from 10 ul bromide tablet containing 2 % agarose gel electrophoresis of 30 minutes ( 5 V / cm ), UV analyzer observed in the 501 bp if occurring at the orange bands for positive samples .