水蛭素

水蛭素基因DNA家族改组方法的建立

Establishment of DNA Family Shuffling Method for Hirudin Genes

B组：生理盐水+水蛭素；

Group B : saline plus hirudin ;

毕赤酵母羧肽酶对重组双功能水蛭素C端的降解作用

The C Terminal of Recombinant RGD-Hirudin Was Hydrolyzed by Yeast Carboxypeptidase

地高辛标记探针检测重组双功能水蛭素中的DNA残留量

Analysis for Residual DNA in Recombinant Double Functions Hirudin Using Digoxin Labeled Probe

PCR方法在水蛭素基因克隆方面的新应用

The new application of PCR method in cloning of hirudin gene

目的应用PCR方法获得水蛭素基因。

PurposeThe aim is to obtain recombinant hirudin gene using PCR method .

结论：用DNA家族改组及亲和淘选技术能够筛选出高活性的水蛭素变异体蛋白，这为其他分子的改组提供了借鉴。

CONCLUSION : Hirudin variants with improved properties could be hopefully aquired by DNA family shuffling and affinity panning .

水蛭素基因的递归PCR合成和在大肠杆菌中的表达与分泌

Synthesis of Hirudin Gene by Recursive PCR and Its Expression and Secretion in Escherichia coli

目的研究重组水蛭素iv给药在大鼠体内的药动学。

Objective To investigate the pharmacokinetics of recombinant hirudin ( r-hirudin ) after iv administration in rats .

抗体夹心酶免疫吸附法测定重组E.coli水蛭素HV3研究

Triple Antibodies Sandwich Enzyme-Linked Immunoadsorbent Assay for Determination of Recombinant E.coli Hirudin ⅲ

新型血栓显像剂～（99m）Tc-重组水蛭素的实验研究

Experimental studies on thrombus imaging with ~ ( 99m ) Tc-recombinant hirudin

水蛭素可抑制ICH后的脑水肿形成，抗凝血酶治疗可能成为ICH后脑水肿的一种可行治疗方法。

Hirudin could inhibit the formation of cerebral edema after ICH .

结论：水蛭素基因DNA家族改组方法的成功建立为进一步筛选高活性的水蛭素奠定了基础。

Conclusion : These results show that a highly shuffled library of hirudin genes has been obtained which lay a substantial foundation for screening hirudin molecules with high bioactivity .

结论实验表明，水蛭素活性因子对S180肿瘤和CAM新生血管具有较强的抑制作用。

ConclusionThe experiments show that hirudin active factors have strong inhibition effect on S180 Tumor and CAM angiogenesis .

重组水蛭素对Beagle犬凝血功能的影响

Effect of recombinant hirudin on coagulant properties in Beagle dogs

重组水蛭素血浆水平依其抗凝血酶活性（TT延长）予以测定。

The rH ( plasma ) level was measured ex vivo by antithrombin activity with TT prolongation as index .

水蛭素合用阿司匹林与氯吡格雷治疗TIA的疗效和卫生经济学比较

Comparison between hirudin with aspirin and clopidogrel for the treatment of TIA in therapeutic effect and hygienic economics

与马尼拉菲牛蛭水蛭素基因Hm1、Hm2的同源性分别为90%与和886%。

The gene sequence had a homology with Hm 1 of 90 % , Hm 2 of 88.6 % from Manila Hirudinaria manillensis .

结果：经过DNA改组和噬菌体亲和筛选，成功获得抗凝血酶活性提高的水蛭素变异体HV2-N47K。

RESULTS : After shuffling and selection , a clone ( HV2-N47K ) with high antithrombotic activity was obtained .

目的探讨水蛭素活性因子对S180肿瘤及血管新生的影响，为临床用药提供理论依据。

ObjectiveTo explore the effect of hirudin active factor on S180 tumor and angiogenesis so as to provide the theoretical basis for clinical medication .

目的：建立毕赤酵母（pichia）高密度表达的重组水蛭素（recombinanthirudin2，rHV2）分离纯化工艺。

AIM : The purpose is to establish the isolation and purification process of recombinant hirudin-2 ( rHV 2 ) expressed in pichia .

方法：利用水蛭素对血栓及血栓前体特异性亲合的作用特点，构建葡激酶与水蛭素C端12肽的融合基因。

Methods : Based upon the specific affinity of hirudin towards thrombin and its precursors , we constructed a fusion gene encoding the protein with a fusion point between the 12 amino acid peptide from the hirudin C terminus and the thrombolytic agent staphylokinase .

目的：1.比较研究普通肝素、低分子肝素、抗凝血酶和重组水蛭素在家兔DIC模型中的抗凝疗效；

Objective : 1 . To study and compare anticoagulation effects of heparin , low molecular weight heparin , antithrombin , recombinant hirudin on disseminated intravascular coagulation .

为检测注射用重组双功能水蛭素半成品中的DNA残留量，应用地高辛标记重组双功能水蛭素工程菌DNA，并以此为DNA探针进行点杂交，同时做特异性及灵敏度实验。

DNA of engineering bacteria of recombinant double functions hirudin were labeled by Digoxin and used as probe for dot hybridization to detect residual DNA in semi-finished products of recombinant double functions hirudin for injection . At the same time , specificity and sensitivity experiments were performed .

FDA在2000年批准了一种新的直接凝血酶抑制剂比伐卢定应用于临床，该药是水蛭素的类似物，它通过抑制凝血酶的活性位点而起效。

Bivalirudin , as a new direct inhibitor of thrombin , was approved by US Food and Drug Administration in 2000 . It is an analogue of hirudin that can inhibit the active site of thrombin .

目的研究水蛭素样肽（HLP）对兔粥样硬化颈动脉成形术后再狭窄的干预作用。

Objective To examine the effect of hirulog-like peptide ( HLP ) on angioplasty-induced restenosis in the carotid arteries of atherosclerotic rabbits .

采用溶氧反馈的分批培养流加补料的方法高密度培养重组大肠杆菌BL21（DE3）生产重组葡激酶-水蛭素融合蛋白。

Recombinant fusion protein staphylokinase-hirudin has been produced by adopting the dissolved oxygen feed-back fed-batch with the high cell density cultivation strategy in E. coli BL21 ( DE3 ) .

目的考察重组水蛭素-2（rHV2）在家兔鼻黏膜中的降解情况。

Aim To investigate the degradation of recombinant hirudin-2 ( rHV2 ) in nasal mucosa of rabbit .

方法采用125-标记的放射性同位素法（RA法）、三氯醋酸沉淀结合放射性检测法（TCA-RA法）研究重组水蛭素iv给药在大鼠体内的药动学。

Methods Two methods , total radioisotope assay with 125I-labeled ( RA ), the radioactivity assay after sample deposited with trichloroacetic acid ( TCA-RA ), were used to detect r-hirudin after iv administration in rats .

构建并表达兼有溶栓和抗凝活性、减少出血副作用的人组织型纤溶酶原激活剂（tPA）和水蛭素（HV2）的融合蛋白。

To combine the fibrinolytic with anticoagulant activities for therapy of thrombotic deseases , a fusion protein made of tissue-type plasminogen activator ( t-PA ) and hirudin was constructed and expressed in Pichia pastoris .