核衣壳蛋白

赤羽病病毒核衣壳蛋白抗体间接ELISA检测方法的建立

Development of an indirect ELISA for detection of Akabane virus infection using recombinant nucleocapsid protein

SARS-CoV核衣壳蛋白抗原性鉴定和基因免疫研究

Antigenicity identification and gene immunization of SARS-coronavirus nucleocapsid protein

汉滩病毒核衣壳蛋白主要B细胞表位的识别及其基因定位

The recognition and mapping of epitopes of Hantaan virus nucleocapsid protein by S gene peptide fragment phage display libraries

核衣壳蛋白基因（N基因）传染性支气管炎病毒的重要结构基因。

Nucleocapsid ( N ) gene is one of the major structural genes of infectious bronchitis virus ( IBV ) .

SARS冠状病毒核衣壳蛋白抑制胞质分裂和细胞增值

Nucleocapsid Protein of SARS Coronavirus Inhibits Cytokinesis and Cell Proliferation

以汉坦病毒重组核衣壳蛋白为抗原进行ELISA血清分型的研究

Use of recombinant nucleocapsid protein of hantavirus as antigen in ELISA for serotyping sera from HFRS patients

结论重组核衣壳蛋白可做为检测SARS抗体的抗原蛋白。

Conclusion The recombinant nucleocapsid protein could be used as an antigen for SARS diagnosis .

经核苷酸序列分析，并与Genbank中的VSV编码核衣壳蛋白N基因序列进行比较。

Compare the sequence with the VSV N gene sequence published in Genbank .

核衣壳蛋白VP39融合TAT转导肽对杆状病毒转导哺乳动物细胞的影响

Tat transduction peptide displayed on the nucleocapsid improved the baculovirus transduction of mammalian cells

根据IBV病毒已报道基因序列设计了一对用于扩增鸡传染性支气管炎病毒T株核衣壳蛋白基因（N）的引物，经RTPCR得到了预期大小的扩增产物；

We designed a pair of primers to amplify the N gene of IBV T strain based on the reported gene sequences .

核衣壳蛋白（NP蛋白）是鸭新城疫病毒结构蛋白中含量最多、保守性高且具有免疫原性的一种蛋白。

The Nucleocapsid protein ( i.e. NP protein ), the highest content of structral protein , has high conservation and immunogenicity .

N蛋白是SARSCOV病毒基因组编码病毒核衣壳蛋白，它不同于现已知的任何蛋白质。

N protein is a nucleocapsid protein encoded by SARS CoV genome , which is different from any other known protein .

结论：SARS-CoV核衣壳蛋白的核定位信号可能位于N蛋白第36-44aa。

Conclusion ; The nuclear localization signal is likely to be located between amino acids 36 and 44 in SARS - CoV nucleocapsid protein .

人源抗汉坦病毒核衣壳蛋白T7噬菌体抗体库的构建

Construction of T7 Phage Display Library against Nucleocapsid Protein of Hantaan Virus

结论：成功克隆SARS冠状病毒核衣壳蛋白基因全长，并在酿酒酵母中诱导表达成功，有助于抗SARS分子疫苗的研究。

Conclusion Successful clone of an integrity SARS-CoV nucleocapsid gene and its effective expression in saccharomyces cerevisiae can be beneficial to the research on SARS vaccine .

目的以汉坦病毒重组核衣壳蛋白（NP）为抗原，对陕西、河北两省部分地区HFRS患者的血清进行ELISA分型。

Aim To make ELISA typing of sera from HFRS patients from partial districts of Hebei and Shaanxi Provinces using hantavirus nucleocapsid protein ( NP ) as antigen .

病毒抗原激活C3的活性，与病毒核衣壳蛋白（nucleocapsidprotein）的含量及毒株对乳鼠的半数致死量有关，并可被特异性抗体（IgG）所中和。

Ability of activating C_3 of EHFV antigens was related to the content of EHFV nucleocapsid protein and LD50 of virus strain to suckling mice , which was neutralized by specific antibody ( IgG ) .

SARS-CoV核衣壳蛋白的体外表达及单克隆抗体功能鉴定

In vitro expression of SARS coronavirus ( SARS-CoV ) nucleocapsid protein and examination of the function of monoclonal antibody induced by the expressed protein

与核衣壳蛋白有关的CTL免疫逃避的研究证实了CTL反应对于控制病毒感染的重要性。

Virus can exploit strategies to evade immune surveillance by CTL associated with NP , that indicates the importance of CTL response in controlling infection of virus .

构建人源T7噬菌体单链抗体（scFv）库筛选抗汉坦病毒核衣壳蛋白（NP）抗体。

A T7 phage display library of human single-chain fragment of variable region ( scFv ) was constructed to screen antibodies against nucleocapsid protein ( NP ) of Hantaan virus .

westernblot印迹结果显示，GST-SCP与PVS抗血清有很强的免疫反应，表明GST-SCP具有天然状态下病毒核衣壳蛋白的抗原性。

The result of western blot shows that GST-SCP can reacts with the antiserum of PVS and GST-SCP has the same immunocompetence as the natural coat protein of PVS .

对重组原核表达载体pETN的表达条件进行了优化，在最佳诱导条件下获得了猪生殖与呼吸综合征病毒重组核衣壳蛋白（N蛋白）。

The expressive condition of the recombinant prokaryotic expression vector pETN was optimized in this experiment . Recombinant nucleocapsid protein of porcine reproductive and respiratory syndrome virus was obtained under the optimum induced condition .

方法：用间接ELISA法检测人和哺乳动物血清SARS冠状病毒（SARS-CoV）核衣壳蛋白（H）和刺突糖蛋白S1片段（S1）IgG滴度，并测定相应抗体的总亲和力。

Methods : Serum samples of healthy contact persons and mammals were detected with indirect ELISA for the titers and relative affinities of SARS-CoV nucleocapsid protein ( H ) and spike glycoprotein fragment 1 ( S1 ) IgG .

用亲和层析纯化的猪繁殖与呼吸综合征病毒（PRRSV）重组核衣壳蛋白GST-N作为诊断抗原，建立检测PRRS血清抗体的间接ELISA方法。

Using recombinant nucleocapsid protein GST-N of porcine reproductive and respiratory syndrome virus ( PRRSV ) as antigen , an indirect ELISA for the detection of antibodies against PRRSV was developed .

目的：建立肾综合征出血热（HFRS）患者汉滩病毒（HTNV）核衣壳蛋白（NP）特异性CTL克隆的靶细胞。

AIM : To establish the target cells of CTL clone which is specific for the nucleocapsid protein ( NP ) of Hantaan virus ( HTNV ) in patients with hemorrhagic fever with renal syndrome ( HFRS ) .

应用RTPCR方法扩增出猪生殖与呼吸综合征病毒（PRRSV）的核衣壳蛋白基因（N基因），并将其克隆到pET32a载体，构建了高效原核表达载体pETN。

The nucleocapsid protein gene ( N gene ) of porcine reproductive and respiratory syndrome virus ( PRRSV ) was amplified by RT-PCR . After the gene has been cloned into pET-32 a prokaryotic vector , a recombinant plasmid pETN was constructed .

从而为ORF7基因及所表达的核衣壳蛋白进一步研究奠定了基础。

This research will lay a foundation for further study on ORF 7 gene and capsid protein expressed by ORF 7 gene .

抗汉坦病毒核衣壳蛋白单链抗体基因的克隆与鉴定

Gene cloning and identification of scFv against nucleocapsid protein of hantavirus

肾综合征出血热病毒核衣壳蛋白在抗感染免疫中的应用

The protective effect of HFRS virus NP in anti - infectious immune

仙台病毒核衣壳蛋白基因的克隆与原核表达线粒体转运核糖核酸亮氨酸基因异常与糖尿病

Cloning and Expression of Nucleoprotein Gene of Sendai Virus in Prokaryotic System