核小体

核小体只提供了DNA压缩的第一级水平。

The nucleosome provides only a first level of condensation of the DNA .

在真核生物中，DNA按左手手征性的方式，缠绕在组蛋白八聚体的周围，形成稳定的核小体结构。

In all eukaryotes , DNA wraps around a histone octamer in a left-handed way , forming a stable nucleosome structure .

基因组DNA包含了绝大部分的遗传物质，对于真核生物而言，DNA缠绕组蛋白八聚体形成核小体，进而被包装在染色质内。

In eukaryote , genomic DNA is packaged in chromatin by twining with histone octamer .

在真核生物中，组蛋白是染色质基本结构一核小体中的重要组成部分，其N末端氨基酸残基可发生乙酰化等共价修饰。

Covalent modifications , such as acetylation occur in the N-terminal amino acids of histones .

钠离子浓度对核小体纤维结构影响的MonteCarlo模拟

Monte Carlo simulation of chromatin fiber conformation affected by ionic strength

染色质修饰包括核小体组蛋白N末端的共价修饰和DNA的胞嘧啶甲基化。

Chromatin modification consists of covalent modifications of the N-terminal tails of the nucleosomal histones and DNA cytosine methylation .

还有实验数据揭示在一个核小体中，组蛋白和DNA相互作用有三个作用很强烈的区域。

Furthermore , experimental data revealed that three regions of strong interactions between core histones and DNA sequences in a nucleosome .

表达核小体Th表位减毒鼠伤寒沙门菌株的构建

Construction and expression of the attenuated Salmonella typhimurium strain expressing the nucleosome Th epitope

DNA作为生命的遗传蓝图，必须被解码或被“阅读”，甚至在被紧密“打包”放进核小体的时候也是如此。

As the genetic blueprint of life , DNA must be deciphered or " read ," even when densely packed into nucleosomes .

这些帮助DNA堆积的蛋白叫做组蛋白，DNA围绕组蛋白形成的基本堆积单位叫做核小体。

These proteins helping DNA package are called histones and the basic element that formed by DNA and histones is called nucleosome .

烟草拟核小体组装蛋白cDNA的克隆、表达及其蛋白产物的纯化

Cloning and Expression of a Putative Tobacco Nucleosome Assembly Protein cDNA and Purification of Its Product

在精子生成过程中，核小体组蛋白被其他更为碱性的蛋白所替代，使DNA的结构变得更为集缩。

During spermatogenesis the histones in the chromatin are replaced by other more basic proteins leading to a more condensed structure of the DNA .

核心过程的表观遗传的产业包括DNA甲基化、组蛋白改性、核小体重建、核染色质动力学和互动子都存在。

Core processes of epigenetic inheritance include DNA methylation , histone modification , nucleosome remodeling , nuclear dynamics and chromatin interaction with non-coding RNAs .

DNA序列上核小体的准确定位在基因转录，mRNA剪接，DNA复制和DNA修复中扮演着重要的角色。

The precise positioning of nucleosomes along the DNA plays important roles in gene transcription , mRNA splicing , DNA replication and DNA repair .

依赖于ATP的染色质改构复合物对核小体结构的调整是基因转录的必要前提。

It is necessary for facilitating transcription to disrupt nucleosome structure by the ATP-dependent nucleosome remodeling complexes .

在真核细胞中，核小体的核心结构组蛋白被DNA紧密缠绕，但是这种紧密的结合有时也可能变得松散，以利于其他蛋白与DNA的结合。

Histone and DNA bind together to form the nucleosome , but this tight binding can loose at some times to facilitate other proteins to interact with DNA .

所有这些信息暗示，DNA序列中存在一个与组蛋白相互作用的模体集合，我们称之为核小体定位模体。

These evidences implied that there was a motif set which were interacted with histone octamers exist on the DNA sequences ; we termed them " nucleosome binding motifs " .

通过分析发现这些模体具有较高的GC含量和柔性，在实验给出的核小体定位序列中，它们出现的频率非常显著。

The analysis revealed that these motifs have higher GC content and flexibility , and their frequencies are significantly high in experimental nucleosome positioning sequences .

这个结果表明，看家基因功能位点附近的核小体定位和重塑信号比Ensemble基因强烈。

This result indicates that nucleosome positioning and remodeling signal near the functional site of housekeeping gene is stronger than Ensemble genes .

核小体动力学主要研究DNA与组蛋白的相互作用下核小体受力、结构形成过程，是蛋白质组研究计划的核心研究内容之一。

Nucleosome dynamics , one of the core areas of histone protein research projects , is largely concerned with the interaction between DNA and histone octamer and properties of the formation of nucleosome complex .

抗核小体抗体及抗C1q抗体在LN发病中起重要的作用。

Anti-nucleosome antibodies and anti-C1q antibodies are suggested to play a role in the pathogenesis of LN .

而未被清除的凋亡细胞会积聚诱发二次坏死，细胞质膜破裂释放出大量内容物，如核小体碎片、DNA、蛋白质、组蛋白成分，引发炎症反应及自身免疫性疾病。

Accumulation of uncleared apoptotic cells results in " secondary necrosis " and the release of cytoplasmic content , such as pieces of nucleosome , DNA , protein , histone composition , causing inflammation and autoimmune diseases .

染色质重构使染色质组织结构发生一系列重要的变化，如染色质去凝集，核小体变成开放式的疏松结构，使转录因子等更易接近并结合核小体DNA，从而调控基因转录等。

Remodeling resultes in a series of important changes in chromatin structure , e.g. loosing the condensed chromatin and opening the structure of nucleosome , which leads to increased accessibility of the transcription factors to nucleosomal DNA .

结论在LN患者中，存在着抗核小体抗体、抗C1q抗体的高表达。

Conclusion In patients with LN , anti-nucleosome antibodies and anti-C1q antibodies are significantly higher than control groups .

分析了人类看家基因功能位点附近的核小体结合模体的分布，并与Ensemble基因比较了它们之间的相同点和差异。

Analyzed the distribution of nucleosome binding motifs near the functional sites of human housekeeping genes and compared the similarities and differences with the Ensemble genes .

在真核细胞中，所有的基因组DNA与高度进化保守的碱性蛋白&组蛋白以及多种组蛋白相关染色质因子组装构成核小体结构，重复此单元而形成染色质结构。

In eukaryotic cells , all genomic DNA with a high degree of evolutionary conservation of basic protein & histone and histone-related chromatin assembly factors constitute a nucleosome structure ; the repetition of this element forms chromatin structure .

随后的一系列功能试验显示，SET/TAF-Iβ是一个具有多种功能的蛋白质，参与到众多的生理途径中。包括组蛋白结合，核小体组装，DNA复制、转录激活以及凋亡等等。

A series of functional tests show that SET / TAF-I β is a multi-functional protein , involved in many physiological pathways , such as the histone binding , nucleosome assembly , DNA replication , transcription and apoptosis .

应用有机汞亲和层析柱分离经微球菌核酸酶有限制消化的HL－60细胞核，得到有机汞层析柱亲和及不亲和的2个核小体组分。

The unbound and bound fractions of nucleosome from HL-60 cell nuclei digested with micrococcus nuclease were separated by Hg-affinity chromatographic column .

儿童系统性红斑狼疮抗核小体抗体和抗dsDNA抗体的检测和意义

Detection and Significance of Anti-nucleosome Antibodies and Anti-dsDNA Antibodies of Systematic Lupus Erythematosus in Children

尽管有许多因素可以影响核小体的定位，但现有的核小体定位数据显示，核小体对特殊的DNA序列有高度的亲和力，DNA本身的序列在体内核小体定位中扮演很重要的角色。

Although nucleosome positioning can be influenced by several factors , present nucleosome positioning data showed that nucleosomes have higher affinity for particular DNA sequences and intrinsic DNA sequence preference plays an important role in nucleosome positioning in vivo .