朊病毒

利用RNAi逆转录病毒载体系统对人朊病毒蛋白表达的稳定抑制

Stable inhibition of human prion protein through a retrovirus-based RNAi system

实验表明，可以利用原核系统取代真核系统表达PrP，并用生物素标记取代35S标记纯化蛋白，建立一个更加安全方便的无细胞构象转化系统，用于朊病毒的研究。

Our study supplies a new system , using more efficiently prokaryotic expressed PrP instead of that expressed in mammalian or insect cells and more easily handling and environmental protecting biotin instead of isotope in cell free conversion for prion study .

ProteinA磁珠检测朊病毒方法的建立

The foundation of Protein A magnetism to detect Prion assay

PrP的胞内运输与朊病毒病

The Role of Intracellular Trafficking of PrP in Prion Diseases

用所设计引物以聚合酶链式反应扩增出不致病的朊病毒蛋白（PrP~c）基因，并克隆到pGEM-Teasyvector。

The PrPc gene was amplified by polymerase chain reaction , using two primers . It was then cloned into pGEM-T Easy Vector .

绵羊朊病毒蛋白基因的原核表达与Ni-NTA纯化

Expression of the ovine PrP gene ( prnp ) in e.coli . and purification in Ni - NTA agarose

朊病毒（prion）具有超强的理化因素抵抗能力，可抵抗常规物理和化学因子对其感染性的灭活。

Prion possesses uncommonly resistant activity to the inactivation of many chemical and physical agents , leading to keeping its infectivity upon the exposures of convenient disinfectants .

中国人群PRNP基因多态性分析及朊病毒新突变分子致病机制的初步研究

Polymorphisms of the PRNP Gene in Chinese Populations and the Pathogenic Mechanism Research of a Novel Insertion Mutant of PrP

朊病毒是Prion病的病原体，朊蛋白（PrPc）是朊病毒在细胞内的正常表现形式，它以GPI锚定的方式定位在细胞膜上，在各物种间高度保守。

The normal , cellular prion protein , designated PrPc , is a glycosylphosphatidylinositol ( GPI ) - anchored membrane protein that is highly conserved in mammalian species .

人的朊病毒病有多种，其中10&15%可以遗传，并与人的朊病毒蛋白基因PRNP的突变有关，另有85%呈散发性。

Human TSEs or prion diseases are described as infectious , inherited , and sporadic form . Approximately 10-15 % of the human prion disease is inherited .

朊病毒病（又称传染性海绵状脑病）的病原是致病型朊蛋白，在牛脑中聚集能够造成牛海绵状脑病（bovinespongiformencephalopathy，BSE）。

A converted form of the normal cellular prion protein ( PrP ) accumulates in the bovine brains with bovine spongiform encephalopathy ( BSE ) . This so-called scrapie-associated prion protein ( PrPSc ) found in many animals and humans is the pathogen of prion diseases .

这说明在这些组织中有正常的PrP存在，为探索Prion致病机理、感染途径提供基础数据，也为朊病毒是否可以垂直传播提供了基础数据。

This suggested that there is normal prion protein existing in these organs . The purpose of the study is to investigate the distribution of prion protein in dairy cattle reproductive system in order to know the prion transmit route and to provide basic data on prion spread .

TSEs由细胞型朊病毒蛋白PrP~C转变成致病型朊病毒蛋白PrP~（Sc）引起，因此也称朊病毒病。

Based on the protein only hypothesis , TSE is caused by the conversion of a normal cellular prion protein ( PrP ~ C ) into a pathogenic , infectious isoform , which has been commonly referred to as scrapie prion protein ( PrP ~ Sc ) .

朊病毒正常功能与致病机理的计算化学研究

Theoretical Study on Normal Function and Conformational Conversion of Prion Proteins

朊病毒蛋白对细胞自噬的影响及可能的作用方式

Effect of Prion Protein on Autophagy and It 's Possible Mechanism

但是，在这些产品里尚未发现朊病毒。

But prions have not been found in these products .

德州驴朊病毒基因克隆与序列分析

Cloning and sequence analysis of prion gene of the Shandong dezhou donkey

皮质畸形与1种罕见的细胞朊病毒蛋白多态性相关

Cortical malformations are associated with a rare polymorphism of cellular prion protein

朊病毒蛋白基因中缩胆囊素反复序列的插入与早发型痴呆的相关性研究

Octapeptide repeat insertions in the prion protein gene and early onset dementia

根据本文报告经病理证实的人朊病毒病14例。

Fourteen cases of human prion disease were pathologically confirmed .

转变医学思维模式看朊病毒之谜

Transforming Medical Thinking Model to Research The Mystery of Prion

朊病毒蛋白密码子129多态性与患阿尔茨海默病的危险性

Prion protein codon 129 polymorphism and risk of Alzheimer disease

毛细管电泳及其在朊病毒检测中的应用

Capillary Electrophoresis and the Detection of the Abnormal Prion Protein

实验动物恒河猴朊病毒基因分析

Analysis of Prion Genes from Laboratory Animals in Rhesuses

中国两种猪朊病毒基因序列的多态性分析

Polymorphisms of the prion protein gene ( PRNP ) in Chinese pig breeds

朊病毒蛋白在胶质瘤中表达的研究

A Study of Prion Protein Expression in Gliomas

东北虎朊病毒基因的克隆与序列分析

Molecular cloning and the sequences analysis of the prion protein gene in Amur tiger

21世纪的库鲁病：一种潜伏期很长的获得性人朊病毒病

Kuru in the 21st century-an acquired human prion disease with very long incubation periods

而且，朊病毒蛋白增强了，淀粉样蛋白的神经毒性。

What 's more , the prions ramp up amyloid beta 's neurotoxic effects .

朊病毒和朊病毒疾病治疗的研究进展

Research Progress on Prion and Prion Disease Therapy