对照细胞

对照细胞对照细胞
  1. 用mRNA差异表达的方法观察镍暴露的人胚肺细胞与对照细胞基因表达的差异。

    MRNA differential display technique was also used to observe the difference in gene expression between exposed and control human embryo lung cells .

  2. MTT法绘制转染细胞及其对照细胞的生长曲线。

    MTT method was used to detect the growth of the transfected cells and control cells . 5 .

  3. 与对照细胞系Kusa-A1/host相比,Kusa-A1/CBF1细胞的钙沉积能力、CN形成能力均显著提高;

    Results In Kusa-A1 / CBF1 cell line , calcium deposition , in vitro mineralization was significantly promoted ;

  4. 以苜蓿转化细胞系和对照细胞系为材料,利用同位素标志方法研究了3种大分子物质&DNA、RNA和蛋白质的合成动态。

    The synthesis of three kinds of macromolecular substances-DNA , RNA and protein in transformed and control cell line of Alfalfa was studied using isotope-tracing methods .

  5. 与对照细胞(野生型细胞W和转导空载体的细胞M)相比,AS细胞在培养中黏附成大团块。

    In comparison to the controls , the wild type cell ( W ) and the cell transduced with the mock ( M ), AS cells formed larger aggregates in culture .

  6. 与肿瘤相关的标志酶β-葡萄糖醛酸酶(β-G)和神经氨酸苷酶(NANase)活力在RA处理下,两项酶活力比对照细胞均显著降低。

    As both β - glucuronidase and neuraminidase activities of them markedly decreased in RA treated cells than in control cells .

  7. 转染尤文肉瘤细胞以及对照细胞后检测DTA表达及其对细胞的杀伤作用。

    After transfection of pS2 DTA into Ewing 's sarcoma cells and the control cells , DTA expression and its killing effect on cells were detected .

  8. 结果:缺氧24h后,对照细胞存活率从缺氧前92%±4%降至1.0%±2.0%;

    RESULTS : After 24-h anoxia , the survival rate of cells was decreased from 92 % ± 4 % before anoxia to 1.0 % ± 2.0 % ;

  9. 测量了用干扰素(3.6×10~7Iu/mgPr)处理的Hep-2细胞和对照细胞的正电子湮没寿命谱。

    Positron annihilation lifetime spectra were measured for the Hep-2 cell cultures treated with the interferon ( 3.6 × 107Iu / mg Pr. ) and control cells .

  10. 经RA处理3天后,细胞染色体的主流范围由对照细胞的4856转变为4450,其中二倍体细胞数由4%上升至15%。

    The major range of chromosome in the cell altered from 48-56 to 44-50 , and the number of diploid was increased from 4 % to 15 % following 3 days RA treatment .

  11. 而bcl-2mRNA表达低于未处理对照细胞,提示药物能通过降低bcl-2表达诱导细胞凋亡。

    And the bcl-2 mRNA expressions are lower than those untreated reference cells , indicating that the medicine is able to induce the apoptosis of RM-1 cells through the lowering of their bcl-2 expressions . 8 .

  12. 同时氯喹可明显抑制IR细胞模型以及对照细胞的EIG及EIP水平,而且抑制的程度与氯喹作用时间的长短密切相关。

    Chloroquine significantly decreased EIG and EIP in IR cells and control cells , and the decreases in EIG and EIP were correlated with the duration of treatment .

  13. Kusa-A1/CBF1细胞OC和OPN基因表达和RANKL蛋白水平明显高于对照细胞。

    The expression level of OC and OPN genes was higher than that in the control cells . Also , Kusa-A1 / CBF1 secreted more RANKL protein than Kusa-A1 / host .

  14. HLFβ细胞自发突变率略有增加,但与对照细胞的差异无显著性,细胞周期分布及细胞恶性程度亦无明显改变。

    No significant change was found between HLF β and the controls in spontaneous mutation frequencies , as well as cell cycle distribution and soft agar growths .

  15. 结果MCF-7/HER2细胞对Taxol,MMC,5-FU,VP-16及TSPA的耐药指数分别为对照细胞的74,22,2.5,3.5和2.8倍,出现了明显的药物抗性(P<0.05);

    Results The resistance index of the MCF-7 / HER2 to Taxol , MMC , 5-FU , VP-16 and TSPA induced were 74,22,2.5,3.5,2.8 times than that in control , respectively ( P < 0.05 );

  16. 这些结果说明IRF-1具有良好的抵抗BVDV作用的生物学功能。(3)研究了IBRV对转基因细胞和对照细胞生物学特性的影响。

    These results indicated well antiviral effect of IRF-1 . ( 3 ) The influence of IBRV on the biological characteristics of transfected cells and the control was evaluated .

  17. 结果HSV-TK蛋白能够在GH3细胞中靶向性表达,予以更昔洛韦后,GH3细胞增殖速度明显减慢,而对照细胞无明显杀伤作用(P<0.01)。

    Results The HSV-TK protein only expressed in GH3 cells . The proliferating rate of GH3 cells treated by Ganciclovir was markedly lowered as compared with control cells ( P < 0.01 ) .

  18. 观察到干扰素作用细胞和对照细胞间O-Ps拾取湮没长寿命成分的明显差别。

    The clear difference of the long-lifetime components related to the pick-off annihilation of ortho positronium between the interferon affected cells and the control cells was observed .

  19. RT-PCR检测显示,TL处理RM-1细胞caspase-3mRNA表达高于未处理对照细胞,提示药物能通过提高caspase-3表达诱导细胞凋亡。

    RP-PCR tests show that , the caspase-3 mRNA expressions of TL treated RM-1 cells are higher than those untreated reference cells , indicating that the medicine is able to induce the apoptosis of RM-1 cells through the heightening of their caspase-3 expressions .

  20. 此外,还观察到12h时ROS显著升高(P<0.01),而在24h时却明显下降,但仍显著高于对照细胞(P<0.01)。

    Moreover , in chronic exposure , Ox-LDL significant induced the increase of ROS at 12 h , but at 24 h ROS levels significantly decreased than at 12 h ( P < 0.01 ) and significantly higher than control cells ( P < 0.01 ) .

  21. 0.1gg/mL衣霉素处理18h后,细胞表面胰岛素受体和胰岛素的结合容量下降,而对照细胞和处理细胞的胰岛素竞争结合曲线基本平行。

    Treatment of 0.1 μ g / mL tunicamycin for 18 hours , the binding capacity of insulin to its receptor of the cell surface was decreased and the competitive binding curve of the treated cells and the control was nearly parallel to each other .

  22. 方法1、用脂质体法,把含有EWS-FLI-1特异结合基因序列的虫荧光素酶报告质粒(pS2)转染到体外培养的尤文肉瘤细胞系(RD-ES、SK-ES)以及对照细胞系(Hela、NIH3T3)。

    Methods 1 . The transfection of luciferase reporter plasmid ( pS2 ), which contains the EWS-FLI-1 binding sequence , into cultured Ewing 's sarcoma cell lines ( RD-ES , SK-ES ) and control cell lines ( Hela , NIH3T3 ) was performed by liposome method .

  23. 流式细胞仪分析转染细胞及其对照细胞的细胞周期分布。

    FCM was used to analyze cell cycle distribution of the transfectants .

  24. 平板集落形成实验检测转染细胞及对照细胞的集落形成能力。

    Colony formation assay was used to investigate the anchor-independent growth ability of transfected cells . 7 .

  25. 裸鼠成瘤实验观察转染细胞及其对照细胞的体内成瘤能力。

    Tumor xenograft in nude mice is performed to test the in vivo tumorigenesis of the transfected HCC cells .

  26. 神经细胞标志物在诱导细胞中的表达水平显著高于其在对照细胞中的水平。

    The expression levels of neural markers in the differentiated cells were significantly higher than those in control cells .

  27. 软琼脂克隆形成实验观察转染细胞和对照细胞在体外的成瘤能力。

    Cloning formation assay on soft agar is applied to test the in vitro tumorigenesis of the transfected HCC cells .

  28. 检测体内筛选获得的实验阳性细胞株的体外培养生长曲线和细胞周期分布等生物学特性,观察可疑变异细胞株的生物学特性与对照细胞株的差异。

    To determine the cell growth characteristics and observe the difference of biological character between the mutated cell clones and the control cell clone .

  29. 同时,膜的相对透性、膜脂过氧化和脱酯化程度的提高幅度也明显低于对照细胞系。

    Meanwhile , the membrane relative permeability , membrane lipid peroxidation and deesterification increased more significantly than those in the salt tolerant cell line .

  30. 此外,具有该突变的细胞能够被用来形成新的克隆四次或更多,而对照细胞不能。

    In addition , cells with the mutation could be used to start new colonies four or more times , while control cells could not .