固体培养

愈伤组织在不含激素的MS固体培养基上可一步分化成完整小植株，在液体培养基中震荡培养可形成大量小块茎。

The callus could differentiate into the plantlets on solid culture and into plenty of tubers on liquid shake culture on MS mediums without plant growth hormone .

在半固体培养基中的AngⅡ则仅刺激cFUGM的形成，却不影响BFUE；

In semi solid culture assay , Ang ⅱ stimulated CFU GM production but no effect on BFU E occured .

白金针菇F3固体培养基优化研究

Study on the Optimization of Solid Medium of Edible Mushroom White Flammulina Velutipes F_3

将人工种子在无激素的MS固体培养基上萌发，萌发率达96%。

The sprouting rate of artificial seed on MS without regulator is up to 96 % .

转接到MS附加多种成分的固体培养基上，愈伤组织得到增殖和生根。

On the modified solid MS media , growth of the calli and root differentiation were observed .

方法骨髓细胞半固体培养和悬浮培养，DNA凝胶电泳，DNA缺口末端标记（TdT），电镜观察。

Methods Semisolid culture , DNA agarose electrophoresis , in situ terminal deoxynucleotidyl transferase assays , view with electromicroscope .

悬浮培养系细胞在液体培养基中比在固体培养基中对Km较敏感。

In suspension culture , cell suspension lines were found more susceptible to Km than on solidified culture .

转化根在不含激素的MS固体培养基上快速生长并表现出典型的毛根性状，经冠瘿碱分析证明确已被转化。

Transformed roots grew rapidly on hormone free MS medium and showed typical hairy root phenotype . Transformation was confirmed by opine analysis .

嫁接苗在MS液体纸桥培养基上的生长量与嫁接成活率显著高于MS固体培养基。

Resultsshowed that growth and survival of in vitro grafts when cultured on MS liquid paper bridge were superior toon MS solid medium .

抗菌活性和最低抑菌浓度（MIC）的测定采用琼脂固体培养法和肉汤液体培养法。

The MICs determinations were performed by solid agar and serial dilution broth methods .

MSC在含有造血因子的半固体培养基中培养4～5周未见造血细胞集落形成。

After 4 to 5 weeks'culture , no hematopoietic cell colony formation was observed from the MSC .

固体培养中低浓度的2，4-D对皂苷的合成有促进作用；

Low concentration of 2 , 4-D can improve saponin content ;

诱导后的组3细胞呈低增殖状态（PI值约为10.4%），在半固体培养基中还可形成血管腔样结构。

The induced cells of group 3 showed low proliferating state ( PI was 10.4 % ) and formed capillary-like structure in semisolid medium .

为了了解重组白细胞介素2对骨髓造血祖细胞体外增殖的影响，应用体外半固体培养法研究了rhIL－2及其与GM－CSF和Epo组合对骨髓祖细胞集落形成的调节作用。

In order to determine the regulation effects of rhIL 2 on hematopoietic progenitor cells from bone marrow .

试验直接以固体培养基上的菌丝体为原料，采用尿素提取液提取菌丝体基因组DNA的方法，耗时短，步骤简捷，提高了效率和安全性。

In this paper , mycelia need not be cultured in liquid medium , and genomic DNA is extracted directly with urea extract . The method requires less time with higher efficiency and greater security .

筛选了一株高产木聚糖酶的黑曲霉（Aspergillusniger）A3菌株，研究了其在固体培养基中的发酵条件。

A high xylanase producing fungus Aspergillus niger strain A3 was isolated and its fermentation conditions by solid state fermentation were studied .

取第3代MSC在含有造血因子的半固体培养基中培养。

MSC were culture in semisolid culture containing hemopoietic growth factor for 4 to 5 weeks to observe the hematopoietic cell colony formation .

将液体培养物稀释到同一梯度，定量接种于固体培养基上（绵羊血琼脂平板和特殊培养基），分别进行需氧和厌氧培养24~48h。

Things of the above cultured , after diluted to the same gradient and inoculated on solid culture mediums quantitatively , were cultured for 24 ~ 48 h under the condition with oxygen and without oxygen .

最适pH值为5.5，其次6.5；固体培养基配方以木屑、梯籽壳作主料，添加适量的麸皮等物为宜。

The most suitable pH value is 5 5 , and then 6 5 It 's solid culture medium obtains wood bits and cottonseed shell , if some wheat bran are added to , it will be better .

麦芽汁培养基、GPY培养基和GPC培养基比较适合于猪苓菌固体培养。

Malt-extract agar ( MEA ), GPC agar and GPY agar were suitable for solid culture to G umbellata .

适宜菌丝体生长的固体培养基是PDA和豆芽汁培养基；利于麻黄生物碱产生的液体培养基以合成培养基为最佳。

It was found that the bean-sprout extract medium or PDA were good as solid media for mycelial growth and the synthetic medium was appropriate to for the production of alkaloid .

前胡的种子幼苗切段在含有1mg/l2,4-D的（1/2）MS固体培养基（大量元素减半）上，诱导产生愈伤组织。

Calli were produced from segments of seedling of Peucedanum dec - ursivum ( Miq . ) Maxim . cultured on the 1 / 2MS agar medium ( with half quantity of macronutrients ) containing 1 mg / 1 2,4-D.

方法：用液体或半固体培养法及流式细胞术测定血小板因子4对CD34阳性脐血细胞的增殖与分化的作用。

Methods : Liquid and semi solid culture systems as well as flow cytometry were used to study the effect of PF4 on the proliferation and differentiation of CD34 + cord blood cells .

30℃固体培养70h，发酵后用无菌水（pH5.0）28℃浸提3h测定酶活性。

The enzyme extraction obtained with sterile water at 28 ℃ for 3h was used for the activity determination .

目的探讨解脲支原体（Uu）在液体培养基中的最佳营养条件和在固体培养基上形成菌落的最佳气体条件。

Objective : To investigate the optimum nutrient conditions of liquid media for growth and the optimum air conditions for colony formation on solid culture media of Ureaplasma Urealyticum ( Uu ) .

微波处理固体培养基120s或过氧化氢质量分数1.0%，微波处理90s，接种培养绿僵菌能够正常生长和产孢。

Metarhizium anisopliae could grow well on the solid medium when treated with microwave 120 seconds or 90 seconds in company with 1.0 % hydrogen peroxide .

微波灭菌的最佳条件为：100g固体培养基加60g水，浸润3h，微波处理150s。

The best technics were 100g solid medium and 60g water were treated with microwave 150s and sterilized entirely after 3 hours soakage before microwave sterilization .

九孔鲍鲍苗脱板过程中细菌胞外产物的研究结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。

Our study showed that during a 24-hour period , higher values are found for the concentration of bacteria , extracellular product and protease activity on the solid media than on the liquid media .

发育成熟体胚转移至含1ppmGA3、0.5ppmNAA和0.25PPmIBA的固体培养基上，发育成完整的小植株。

On the solidified medium with 1 ppM GA_3 , 0.5 ppM NAA and 0.25 ppM IBA , the mature somatic embryos could develope into healthy plantlets .

固体培养最佳时间为20d。液体菌种接种量为4%，初始pH为6~7，最佳培养温度为26℃。

The results showed that the optimum pH ranged from 6 to 7 and the optimum temperature was 26 ℃ with 65h of liquid culture , 20 days of solid culture and 4 % of initial inoculum .