四唑

运用MNDOMO法对五种四唑衍生物的互变异构反应进行了理论计算。

Theoretical calculations on the tautomerization of five tetrazole derivatives are performed with MNDO MO method .

四唑及其衍生物的理论研究（9）&腈基四唑衍生物的ABINITIO和热力学计算

Theoretical Study on Tetrazole and Its Derivatives ( 9 ) & ab initio MO and Thermodynamic Calculations of Cyano Derivatives of Tetrazole

N′-5-四唑基-N-芳甲酰基硫脲的合成及其生物活性研究（I）

Synthesis and Biological Activity of N ′ - 5-Tetrazolyl-N - aryl Formyl Thiourea ( I )

采用四唑盐比色法（MTT）测量细胞增殖。

VSMC proliferation was measured by tetrazolium salt ( MTT ) methord .

方法：采用体外细胞培养技术和四唑盐显色（MTT）方法。

Methods : Using colorimetric MTT ( tetrazolium ) assay in vitro .

方法：药物敏感试验采用四唑氮盐（MTT）比色试验法。

Methods : Cytotoxic effects were determined with MTT colorimetry .

5-氨基四唑Schiff碱的合成与生物活性

Synthesis and biological activity of Schiff base of 5 - amino - tetrazole

方法：采用四唑蓝（MTT）比色法、平板集落形成实验以及[3H]-TdR掺入实验。

Methods MTT assay , colony forming experiment and [ 3H ] TdR incorporation into DNA were used .

方法采用MTT（四唑氮蓝）法检测不同浓度的氨基葡萄糖硫酸盐对K562细胞生长的影响；

METHODS MTT was used to test the growth curve of K562 and K562 induced by glucosamine sulphate .

四唑盐比色试验（MTT）比较正常和缺氧状态下牛视网膜血管内皮细胞的细胞数目。

By using MTT , we described cell number of retina microvascular endothelial cell under normal and hypoxic condition .

目的：采用四唑盐（MTT）药敏试验方法筛选对肺癌的敏感药物并指导临床化疗。

AIM : To screen sensitive drugs to lung cancer for clinical chemotherapy by using MTT drug sensitive test .

基于四唑乙酸的二维Ce（III）配合物的合成、结构和光学性能研究

Synthesis , Crystal Structure and Property Study of Ce ( III ) Compound Based on 2 - ( 1H-Tetrazol-1-yl ) Acetic Acid

方法采用甲基四唑蓝法（MTT）测定柔红霉素（DNR）的细胞毒性；

Methods The cytotoxicity of daunorubicin ( DNR ) was assayed by MTT method .

用H2O2模拟心肌细胞的氧化应激，用四唑盐比色试验（MTT法）检测H2O2处理后不同时间点的线粒体酶活性；

After cells were treated , the MTT assay was used to measure the activity of mitochondrial enzyme in cardiomyocytes .

培养12、24、48h用二甲基噻唑二苯基四唑溴盐（MTT）法测定细胞增殖速度；

After culture of 12,24 or 48 h , the cell proliferation rate was measured by MTT colorimetric determination .

方法：用四唑盐（MTT）法检测125I粒子近距离照射对BEL-7402细胞增殖的影响；

Methods The proliferation of BEL-7402 cell was measured after 125I seed brachytherapy using MTT method .

方法：反复冻融法提取肿瘤可溶性抗原，甲基噻唑基四唑（methylthiazolylterrazolium，MTT）法检测效应细胞杀伤活性。

Methods : Soluble antigen was prepared from tumor cells by repetitive freeze thaw method . Cell killing activity is measured by MTT .

方法：采用细胞培养技术及四唑盐（MTT）比色方法，检测加入SDS后与对照组比较细胞活性的变化。

METHODS : The cell culture technique and MTT method were used to observe the effect of SDS on the activity of PDLC .

方法：用氮蓝四唑（NBT）光还原法测定O2·的清除率，氧化还原法测定·OH的清除率。

Methods : Measure the content of superoxide free radical and hydroxyl free radical by nitro blue tetrazolium ( NBT ) photo-reduction .

方法：采用四唑盐（MTT）比色实验、集落形成实验为指标观察哌唑嗪对K562细胞增殖作用的影响；

Methods The effects of prazosin on the proliferation of K562 cells were evaluated by liquid culture assay , MTT assay and colony formation assay .

方法采用（1）硝基蓝四唑（NBT）试验测定WBC的自发活化率（SAR）；

Methods ( 1 ) Nitro blue tetraxolium test was used to measure the spontaneous activating rate ( SAR ) of circulating leukocytes .

该匀相比色检测法是基于一种淡黄色底物四唑盐MTT可转化生成甲臜，甲臜是一种紫色显色剂。

This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT , a pale yellow substrate , to formazan , a purple dye .

采用四唑盐（MTT）比色法测定细胞活性、吖啶橙荧光染色观察细胞凋亡的形态学变化，WESTERNBLOTTING分析Bcl-2、Bax和caspase-3蛋白的表达。

Cell viability was determined by MTT assay , apoptosis by acridine orange fluorescence dyeing , and expressions of Bcl-2 、 Bax and caspase-3 were analyzed by Western blotting .

用四唑盐（MTT）法检测细胞增殖情况，用碱性磷酸酶（ALP）试剂盒检测ALP活性，用细胞免疫实验检测细胞外基质中羟脯氨酸浓度，观察细胞胶原蛋白的合成分泌。

Cell proliferation was measured by MTT tese while activity of alkaline phosphatase ( ALP ) and hydroxyproline concentration in cell matrix was checked by ELISA .

经6、24、48和72h后，用四唑蓝（MTT）法测定细胞增殖率、细胞匀浆中癌胚抗原（CEA）及和碱性磷酸酶（ALP）的表达；

The cell proliferation rate was measured by MTT and expression of CEA and ALP were detected after 6 , 24 , 48 and 72 h.

同时设对照组。照射后48h采用四唑盐（MTT）比色法和激光共聚焦成像技术，分别测定细胞线粒体功能及细胞内游离Ca2+。

48 hours later , the cellular mitochondria function and cytoplasmic Ca 2 + concentration were assessed by means of MTT method and confocal microscopy respectively .

为了研究血清胆固醇对循环白细胞活化作用的影响，采用硝基蓝四唑试验测量了高脂血症家兔循环白细胞自发活化率（SAR）。

To study effect of serum cholesterol on activation of circulating leukocyte , Nitro blue tetrazolium test was used to measure the spontaneous activating rate ( SAN ) of circulating leukocyte in hyperlipidemic rabbits .

分别于诱导细胞移植后第2周及第8周，进行网屏握持试验、抓握牵引试验、平衡木行走试验及Morris水迷宫测试，并取脑组织做2,3,5-三苯四唑化氯（TTC）染色。

Screen test , beam test , prehensile traction test and Morris maze test were conducted at 2 and 8 weeks alternately after transplantation .

目的：探讨MTT、MTS、WST-1三种四唑盐在细胞增殖检测中的最佳实验条件。

Objective : To investigate the optimal experiment conditions of MTT , MTS and WST in cell proliferation assays .

方法：采用四唑蓝（MTT）比色法检测8株消化道肿瘤细胞株对EGCG敏感性的差异；

Methods : Methyl thiazolyl tetrazolium ( MTT ) analysis was used to detect the discrepancies of eight digestive tract cancer cell lines ' sensitivities to EGCG .