卵母细胞

RNA干扰在哺乳动物卵母细胞及早期胚胎发育研究中的应用

Application of RNA Interference in the Study of Mammalian Oocyte and Early Embryo Development

此外，我们目前对卵丘细胞促进卵母细胞成熟质量的机制还知之甚少。因此，建立一种完善的DOs体外成熟系统具有非常重要的应用价值和理论意义。

In addition , the mechanisms by which cumulus cells improve oocyte maturation are poorly understood .

用激光共聚焦显微术在小鼠卵母细胞中检测蛋白激酶C

Detection of Protein Kinase C by Confocal Laser Microscopy in Mouse Oocyte

B级为卵丘细胞1～3层，基本上包裹卵母细胞；

Grade B have 1-3 layers fully cumulus cells ;

钙对人卵母细胞皮质颗粒排放的调节及与经典型蛋白激酶C关系

The effect of conventional protein kinase C on the cortical granule exocytosis of human oocyte induced by calcium

蛋白激酶C和MAPK/p90rsk对小鼠卵丘-卵母细胞复合体减数分裂成熟的调节

Regulation of Protein Kinase C and MAPK / p90rsk Cascade on the Induced Meiotic Maturation in Mouse Cumulus Cell-Oocyte Complexes

卵泡FSH受体mRNA的表达及其对卵母细胞体外培养成熟率的影响

Expression of FSHR mRNA and Its Effect on Porcine Oocytes Maturation in Vitro

蛋白激酶C（PKC）被认为是在哺乳动物受精期间卵母细胞激活的主要调节者。

Protein kinase C ( PKC ) has been proposed to regulate major egg activation events during mammalian fertilization .

把提取的鼠脑mRNA注射到卵母细胞内，在卵母细胞上以双电极电压钳技术研究某些递质的诱导电流。

Two electrodes voltage clamp technique was performed to investigate neurotransmitter induced currents after mouse brain mRNA was injected into Xenopus oocytes .

方法微量注射仪将纯化的大鼠脑mRNA注入准备好的爪蟾卵母细胞内并于19℃温浴48h以上使其表达；

Methods mRNA was isolated from brain of neonatal rat and injected into prepared Xenopus oocytes .

培养液中不添加血清与添加血清时卵母细胞成熟率分别为60.3%和33.8%，差异极为显著（P＜0.01）。

With or without serum in the maturation medium , the porcine oocyte IVM rate was 33.8 % and 60.3 % , respectively , with significant difference ( p < 0.01 ) .

以PVS沉淀法和荧光免疫检测法，考察爪蟾V、V工期卵母细胞是否有内源性的LDLR。

㏕ o investigate whether Xenopus Oocyte have endogenetic LDLR with PVS Sedimentation and Fluorescence detection .

PCOS大鼠窦状卵泡卵母细胞超微结构变化

Ultrastructure of Oocytes in PCOS Rat Antral Follicles

用离子霉素联合6-DMAP能有效地激活绵羊卵母细胞，其囊胚率为30.4%，高于细胞松弛素B法的27.4%；

Matured sheep oocytes were efficiently activated by Ionomycin together with 6-DMAP and 30.6 rate of blastocyst were achieved .

结论1、线粒体ATP生成减少与卵母细胞受精失败、受精卵未卵裂及胚胎发育异常可能有关。

Reduced ATP content may be related to failures in oocyte fertilization , zygote division and abnormal embryo development . 2 .

添加FSH的猪卵母细胞成熟培养效果优于hMG；

FSH is more efficient for in vitro maturation of porcine oocyte than hMG ;

FSH诱导小鼠卵母细胞体外成熟机制的研究蓝狐卵母细胞的体内外成熟

Mechanism of Mouse Oocyte IVM Induced by FSH Study on the Maturation in vitro of Blue Fox Oocyte

表皮生长因子EGF与未成熟卵母细胞体外成熟的相关研究

Significance of Epidermal Growth Factor ( EGF ) in the In-Vitro Maturation of Immature Oocytes and the Correlation Analysis

电刺激激活处理卵母细胞显著提高了精子头核和死精子注射后卵子的受精率（13.79%vs.19.30%；12.50%vs.18.97%；P＜0.01）。

The rate of fertilization of sperm heads and dead sperms was significantly increased by the electric simulation treatment of ova ( 13.79 % vs.19.30 % ; 12.50 % vs.18.97 % ; P < 0.01 ) .

结果表明克隆牛的微卫星DNA与核供体牛完全一致，而线粒体DNA则全部来源于卵母细胞。

The results of the present study indicate that the microsatellite DNA of cloned cattle is consistent with the nuclear donor absolutely . However , their mtDNA is originated from corresponding oocytes completely .

用荧光染料（Hoechst33342）和激光处理精子对牛卵母细胞体外受精的影响

Effects of Hoechst 33342 and laser on bovine in vitro fertilization

进一步检测发现Cofilin1敲除后减数分裂进程受到了显著的影响，更多的卵母细胞被阻滞在前MI期和MI期。

Further more study found the meiosis process was influenced significantly , more oocyte was arrested at pre-MI and MI of meiosis .

系统探讨了放线菌酮（CHX）和细胞松弛素B（CB）对水牛卵母细胞孤雌激活的影响。

Effects of cycloheximide ( CHX ) and cytochalasin B ( CB ) on the parthenogenetic development of buffalo oocytes were investigated .

ANP和C-ANP-（4-23）对猪卵母细胞自发成熟没有影响。

Both of ANP and C-ANP - ( 4-23 ) had no effect on spontaneous porcine oocyte maturation and cumulus expansion .

结果：TGFα、EGFR在所有胎儿卵巢卵母细胞、部分卵泡细胞及基质细胞呈阳性表达，在卵母细胞的表达均强于卵泡细胞与基质细胞。

Results : TGF α and EGFR were expressed in all of the oocytes in the fetal ovaries , partly in follicular cells and stromal cells .

MII期卵母细胞质量对卵胞浆内单精子注射结局的影响

The impact of MII oocyte morphology on the outcome of intracytoplasmic sperm injection

目的探讨年龄和月经周期时相对不成熟卵母细胞质量及其体外成熟（InVitroMaturation，IVM）能力的影响。

Objective To investigate the effects of donor 's age and retrieval menstrual cycle phase on the quality of immature oocytes and the results of in vitro maturation .

研究了FCS，17β－E2，FSH，LH对山羊卵泡卵母细胞成熟的作用，及培养时间对卵母细胞核和细胞质成熟的影响。

The effects of FCS , culture time , 17 β estradial , FSH and LH on goat oocytes in vitro maturation were studied .

乙醇及6-DMAP对小鼠卵母细胞孤雌激活的研究

Parthenogenetic activation of mouse oocytes by ethanol and 6-DMAP

作者就PKC在卵母细胞第1次减数分裂成熟过程中的作用综述如下。

Here in this article , the recent advances of research in PKC in the process of Meiosis ⅰ during mammalian oocyte maturation is reviewed .