卫星细胞

VEGF基因体外转染肌卫星细胞的研究

Preliminary Study on Muscle Satellite Cell Transfected with VEGF in vitro

急性运动对大鼠骨骼肌卫星细胞增殖能力和不同pH值对骨骼肌细胞胞浆Ca～（2+）代谢的影响

Effect of Acute Exercise on the Satellite Cells and Cytoplasmic Ca ~( 2 + ) Metabolism of Rat Skeletal Muscles

观察细胞各个阶段的形态，通过生长曲线等手段研究骨骼肌卫星细胞的增殖与分化能力，利用RT-PCR以及免疫细胞化学染色对所获得的细胞进行鉴定。

Morphological observation and growth curve were used to evaluate the proliferative and differentiation ability of skeletal muscle satellite cells .

ACh可刺激卫星细胞形成的肌管收缩。

Adding ACh directly in the medium stimulated the contraction of myotubes .

NGF、BDNF和NT-3在背根节卫星细胞的表达

Expression of NGF , BDNF and NT-3 in Satellite Cells of Dorsal Root Ganglion

观察NGF、BDNF和NT-3及其mRNA在DRG卫星细胞的分布以及亚细胞定位。

Results NGF , BDNF , NT 3 and their mRNA were observed in some satellite cells .

GFP转基因鼠咀嚼肌卫星细胞的培养及影响因素

The Culture and Influence Factors on Proliferation of Skeletal Muscles Satellite Cells from GFP Transgenic Mouse in vitro

应用半定量PCR及WESTERNBLOTTING测定骨骼肌卫星细胞中结蛋白基因及结蛋白含量的表达。

Applicating semi-quantitative PCR and Western blotting to measure the expression of desmin gene and desmin by skeletal muscle satellite which are interfered by different concentrations of androgen .

体外培养的SC对肌卫星细胞及大鼠失神经支配早期骨骼肌、运动终板的作用研究

The Study on the Role of Schwann Cells Cultured in Vitro to Myoblast and Early Denervated Skeletal Muscle and Endplate in Rats

部分背根切断后备用DRG卫星细胞NGF、BDNF和NT-3的表达变化

The expression changes of ngf , BDNF and NT-3 in satellite cells following partial dorsal root rhizotomy

EGF、bFGF和PHGF对大鼠骨骼肌卫星细胞增殖的影响

The Effects of EGF , bFGF and PHGF on the Growth of Rat Skeletal Muscle Satellite Cell

在这些移植细胞中，骨骼肌干细胞（卫星细胞，SC）占有很重要的地位。

Among these muscle cells , skeletal muscle stem cell ( satellite cell , SC ) plays an important role in cell transplantation .

EGF、TGF-β及胰岛素对兔骨骼肌卫星细胞增殖的影响

The effects of EGF , TGF - β and insulin on the growth of rabbit ′ s skeletal muscle satellite cell

EGF，TGF-β和Insulin对兔骨骼肌卫星细胞增殖、细胞周期及其功能代谢的影响

The effect of egf , tgf - β and insulin on the growth , cell cycle and metabolism of cultured rabbit 's skeletal muscle satellite cells

针刺对备用背根节卫星细胞NGF，BDNF和NT-3表达的影响

The Expression of NGF , BDNF , NT-3 and Their mRNA in Satellite Cells of Spinal DRG Following Acupuncture

bFGF置入失神经骨骼肌对肌卫星细胞增殖与肌萎缩影响的实验研究

An Experimental Study on Placing bFGF at the Position of Denervated Skeletal Muscle to Affect the Muscle Satellite Cell Proliferation and the Muscle Atrophy

在分化培养过程中，观察肌卫星细胞形态，计算其融合率，应用半定量RTPCR技术检测细胞总RNA中肌细胞生成素mRNA的表达量。

During differentiation , the morphology of muscle satellite cell was observed and the fusion rate was calculated under microscopy . The level of myogenin mRNA expression was checked by semi-quantitative RT-PCR .

GDNF和HSV-GDNF对体外培养发育的大鼠骨骼肌卫星细胞Bcl-2表达的影响

Effects of GDNF and hsv-gdnf on bcl-2 expression of the development of muscle satellite cells of newborn rat in vitro

目的探讨应用改良Percoll非连续密度梯度离心法分离纯化培养组织工程用肌卫星细胞。

Objective To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique .

有在体研究表明，机械生长因子（MGF）可能能激活骨骼肌卫星细胞，并促进其增殖。

Studies have shown that MGF appears to initiate muscle satellite cell activation and proliferation in vivo .

方法原代培养骨骼肌卫星细胞，经两次传代后，取培养2、4、8、16、24、48、72h的细胞行SCF单克隆抗体的免疫组织化学染色。

Methods The skeletal muscle cells were cultured for 2,4,8,16,24,48,72 hours individually . After the second passage , these cells were stained with SCF monoclone immunohistochemical staining technique .

目的研究肌细胞生成素（myogenin）在人体肌卫星细胞分化培养中的表达规律，探索肌细胞生成素在肌卫星细胞分化中的作用。

Objective To investigate the expression rule and its function of myogenin mRNA during differentiation of human skeletal muscle satellite cells .

目的研究骨骼肌卫星细胞梗死心肌移植的胰岛素样生长因子（IGF）1、碱性纤维母细胞生长因子（bFGF）分泌及血管再生的作用。

Objective To study the insulin like growth factor 1 ( IGF 1 ), basic fibroblast growth factor ( bFGF ) secretion and vascular regeneration by the skeletal muscle satellite cells implanted into infarction myocardium .

【方法】分别采用差速贴壁法和Percoll非连续密度梯度离心法进行新西兰兔肌卫星细胞的原代培养并进行纯化。

[ Methods ] The primary skeletal muscle satellite cells of New Zealand white rabbits were cultured with different adhesion time method and incontinuous Percoll density gradient centrifugation technique .

卫星细胞无明显的N-ACh受体表达；

No obvious expression of ACh receptors was observed on the surface of satellite cells .

【结果】两种方法均能分离培养获得肌卫星细胞，经测定差速贴壁法所得细胞纯度为30-40%，Percoll非连续密度梯度离心法所得细胞纯度在90%以上。

[ Results ] Over 90 % satellite cells were harvested by incontinuous density Percoll gradient centrifugation technique , in contrast to which , only 30-40 % cells were harvested by different adhesion time .

观察BMP对骨骼肌卫星细胞粘附、增殖及分化等生物学特性的影响，以探讨带血供肌瓣作为BMP载体修复骨缺损的细胞生物学机制。

To investigate the effects of bone morphogenetic protein on adhension , proliferation and differentiation of skeletal muscle satellite cells so as to interpret the biological mechanisms of vascular muscle flap as a carrier of bone morphogenetic protein to repair bone defect .

结果发现，部分去背根术后7d和10d组L6DRG的NGF、BDNF和NT-3的阳性卫星细胞数量较术后3d组和正常组者明显增高（P<0.01）。

The results showed that the numbers of satellite cells for NGF , BDNF and NT-3 on 7 days and 10 days after operation increased more apparently than that of the intact group as well as 3 days group after operation ( P < 0.01 ) .

小鼠骨骼肌卫星细胞的原代培养和鉴定

Primary cultivation and identification of satellite cells of mice skeletal muscles

结论酶消化法适用于卫星细胞的培养。

Conclusion This methods was suitable for collection skeleton satellite cell .