免疫组织化学法

在再灌注24h断头取脑组织，采用免疫组织化学法检测脑组织中Caspase-3蛋白的表达，并与病理组织学改变进行对照。

The Caspase-3 expression in brain tissue was measured with immunohistochemical method and was compared with the change of pathohistology .

组1、2以生理盐水代之。术后24h取左侧睾丸标本分别以原位缺口末端标记法（TUNEL）及免疫组织化学法检测凋亡细胞和Bcl-2、Bax基因蛋白表达。

24 h after operation , left testis was removed and assessed for cell apoptosis and expressions of Bcl-2 and Bax by TUNEL technique and immunohistochemical method respectively .

行血清转氨酶及免疫组织化学法测定肝细胞内NFκB。

The AST , ALT level and the expression of NF kB were detected by immunohistochemistry method .

用免疫组织化学法检测气道黏膜炎症细胞Fas的表达。

The expression of Fas in tracheal inflammatory cells was tested by immunohistochemical method .

免疫组织化学法观察心、肺、肾的VEGF表达。

The expression of VEGF in tissues of heart , lung and kidney were observed by immunohistochemistry staining .

同时收集胎盘组织，以酶免疫组织化学法检测乙型肝炎病毒感染的孕妇胎盘组织中的HBSAg。

Their placental tissue was collected to detect HBsAg in the cells by immunohistochemical method too .

免疫组织化学法检测了海马组织iNOS的表达。

Immunohistochemistry method was used to detect the iNOS expression in hippocampal tissue .

用免疫组织化学法检测各组的C3、IgG和CD68。

C3 , IgG and CD68 were determined by using immunohistochemical method .

人体角蛋白PAP免疫组织化学法

A PAP Immunohistochemical Staining Method for Demonstration of Human Keratin

并用HE染色观察脑组织病理学变化，免疫组织化学法检测caspase-3凋亡蛋白表达的变化。

Immunohistochemical method was used to investigate the expression levels of caspase-3 in brain tissue .

结果：通过免疫组织化学法鉴定此抗-Aβ42多克隆抗体，结果显示在AD患者的脑组织淀粉样沉淀物中呈阳性。

Results In immunohistochemical studies the polyclonal anti-A β 42 antibody showed strong positive staining of amyloid deposits in brain tissues from AD patients .

免疫组织化学法及Western印迹法检测肾组织中WT1和Bcl-2蛋白质表达。

Immunohistochemistry and Western blot were used to examine the expression of WT1 and Bcl-2 gene products in renal tissue .

免疫组织化学法分析DRGⅡ对K562细胞Bcl-2、Bax、Fas蛋白表达的影响。

Expressions of Bcl-2 , Bax and Fas proteins were analyzed with immunohistochemical staining in K562 cells .

方法以抗TIMP1和TIMP2单克隆抗体及TIMP1和TIMP2cDNA探针为试剂，采用免疫组织化学法和原位杂交技术检测肝硬化组织中TIMP1和TIMP2蛋白和mRNA。

Methods TIMP 1 and TIMP 2 proteins and mRNA were detected with immunohistochemistry and in situ hybridization methods using monoclonal antibodies and cDNA probes .

用免疫组织化学法检测胰腺癌中增殖细胞核抗原（PCNA）的表达，并观察两者的关系。

Meanwhile , the expression of proliferative cell nuclear antigen ( PCNA ) was detected by immunohistochemical assay .

同时采用免疫组织化学法检测流产者胚胎组织中表皮生长因子受体（EGFR）表达程度。

Expression of EGF receptor ( EGFR ) in the abortus was studied with immunohistochemical method .

用免疫组织化学法检测CD4，CD8，CD57和CD68在移植物中的沉积。

CD4 , CD8 , CD57 and CD68 were detected by immunohistochemistry .

用免疫组织化学法对人胎胃粘膜NOS阳性细胞的表达进行观察。

The development of NOS positive cells is connected with the surface epitheliums and glands of gastric mucous closely .

免疫组织化学法检测肝组织HBsAg和乙型肝炎核心抗原（hepatitisBcoreantigen，HBcAg）。

In addition , viral specific proteins , HBsAg and hepatitis B core antigen ( HBcAg ), in the liver were assayed by immunohistochemical staining .

并将转基因肿瘤细胞接种于裸鼠体内，应用免疫组织化学法检测肿瘤内微血管密度（MVD），探讨MVD与肿瘤生长转移的关系。

Microvessel density ( MVD ) in xenografted tumors in nude mice was analyzed by immunohistochemistry .

采用免疫组织化学法和图像分析技术检测卵巢GnRH受体。

Ovarian GnRH receptor was detected by immunohistochemical method .

方法：采用大鼠切口疼痛模型，用累积疼痛评分法评价疼痛行为及免疫组织化学法观察P物质的表达。

Methods : Using a rat model of postoperative pain , the cumulative pain score ( CPS ) was measured to evaluate painful behaviors and immunohistochemistry technique was used to determine the expression of SP.

采用ABC免疫组织化学法对60例肺未分化癌进行p53蛋白阳性表达的研究。

The expression of tumor suppressor gene p53 in 60 patients with primary undifferentiated lung cancer was studied with ABC immunohistochemical technic .

免疫组织化学法检测癌细胞Caspase3、Bcl2、p53表达。

The cellular expression of Caspase-3 , Bcl-2 and p53 were observed by immunohistochemistry method .

用免疫组织化学法检测瘤组织中PCNA及Bcl-2蛋白的表达。采用原位末端标记法检测瘤组织中的凋亡细胞。

Expression of PCNA and Bcl-2 in carcinoma tissues was detected by immunohistochemistry , and apoptosis of cells were labeled with TUNEL .

福尔马林处理后2h，免疫组织化学法检测大鼠脊髓后角CFos蛋白的表达。

At 2 h after injection of formalin , the expression of C-fos protein in spinal dorsal cord was detected using immunohistochemical method .

用免疫组织化学法，在冰冻切片上观察了不同胎龄组肝内前b细胞的形态、分布及其分化为b细胞过程中抗原的变化。

The morphology and distribution of pre-B cells and the changes of antigens during the differentiation from pre-B to B cells in fetal livers of different gestational ages were studied by means of immunohistochemical technique .

免疫组织化学法检测凋亡相关蛋白Bcl2、Bax的表达；

The expression of Bcl2 and Bax protein in myocardium was observed by immunohistochemical method .

用免疫组织化学法显示心肌缺血区、边缘带及非缺血区Cx43的分布。

The distribution of Cx43 in the ischemia myocardium was studied by immunohistochemistry technique .

运用流式细胞仪检测大鼠肝细胞凋亡，免疫组织化学法检测Bcl-2、Bax基因蛋白表达。

Flow CytoMeter and immunohistochemistry technique were used to detect the apoptosis of hepatocytes and the expression of Bcl-2 , Bax in hepatocytes respectively .