体外翻译

棉铃虫核型多角体病毒mRNA的快速制备与体外翻译

Rapid Purification and in vitro Translation of Heliothis armigera Nuclear Polyhedrosis Virus MRNA

反义核酸抑制抗敌百虫淡色库蚊扩增酯酶mRNA体外翻译的研究

Inhibition of in vitro Translation of Esterase mRNA of Dipterex-Resistant Mosquito ( Culex pipiens pallens ) by Antisense Nucleic Acids

在体外翻译体系中检测N蛋白对体外翻译的影响。

The effect of N protein in vitro was examined in an RRL system .

小麦醇溶蛋白mRNA的分离及体外翻译

Isolation and translation in vitro of gliadin mRNA of Wheat

小鼠腹水瘤病毒RNA的提纯和体外翻译

The purification of SRSV RNA and its translation in vitro

兔胰mRNA的制备与有胰岛素免疫活性多肽的体外翻译

Preparation of pancreatic mRNA from rabbit and translation of insulin - immunoreactive polypeptide in vitro

免疫RNA体外翻译抗体的研究

Antibody Translation of I - RNA in Vitro

铜IUD对兔子宫内膜mRNA体外翻译的影响

Effect of Copper IUD on in-Vitro Translation of Rabbit Endometrial mRNA

光对小麦幼苗poly（A）mRNA及其体外翻译产物的影响

Effect of Light on poly ( A ) mRNA and Its Translation Products in Vitro of Wheat Seedling

可用体外翻译获得的可溶天然型EGFP的表达

The prokaryotic expression of soluble native EGFP may be obtained by Cell - free translation system

应用网织红细胞裂解体外翻译及免疫共沉淀试验，进一步验证CD81分子与HBeAg的结合。

The binding of HBeAg to CD81 was also detected in vitro translation and coimmunoprecipitation test .

采用多聚尿苷酸-琼脂糖柱层析，从总的RNA中分离到88μg总的mRNA，免网织红细胞溶胶体外翻译体系产物表明，分离的mRNA具有生物功能。

Applied poly ( U ) - sepharose column , 88 μ g total mRNA was got from total RNA . The in vitro products for rabbit reticulocyte lysate translation system showed that isolated mRNA possessed biological function .

TLSF（JM）mRNA体外翻译的成功为其CDNA文库的构建以及TLSF（JM）基因克隆和表达提供了重要的理论依据和实验基础。

Success in TLSF_ ( JM ) mRNA translation in vitro might provide the basis for establishment of cDNA library and TLSF_ ( JM ) gene cloning and expression .

同时，将突变体克隆至PGEM-CAT-LUC载体中，利用TNT？QuickCoupledTranscription/TranslationSystems体外翻译试剂盒，在体外条件下分析这些重组质粒LUC基因的表达。

Meanwhile , cloned the mutants into the PGEM-CAT-LUC vector , using TNT ? Quick Coupled Transcription / Translation Systems , analyzed the expression of LUC gene of these recombinant plasmid in vitro .

结论：能方便地获得TTF1体外翻译蛋白，为进一步研究TTF1蛋白与相应的DNA反应元件及其他转录因子的相互作用奠定了基础。

Conclusion : Expression of TTF1 in vitro can be used in the further research of interaction of TTF1 and its DNA response elements and others transcription factors .

并在变性条件下经琼脂糖凝胶电泳鉴定，证明它是9～10S的RNA。人珠蛋白mRNA在兔网织红细胞溶解物与麦胚体外翻译体系中的翻译活性与其浓度均有依赖关系。

The result of agarose gel electrophoresis under unnatural conditions revealed that the S value of mRNA was 9 to 10 . In both reticulocyte lysate and wheat germ cellfree system , the globin mRNA showed a high translation activity depending on its concentration .

方法人工合成互补于抗性库蚊酯酶mRNA翻译起始点18碱基，与淡色库蚊mRNA退火后加入无细胞翻译体系，进行体外翻译，翻译产物用SDS聚丙烯酰胺凝胶电泳进行分析。

Methods 18 mer nucleic acid was synthesized and complementary to the translation initiation site of mRNA of dipterex resistant mosquitoes . The ODNs were annealed to the corresponding mRNA molecules and they were added to rabbit reticulocyte cell free system . The translation products were analyzed by SDS PAGE .

人珠蛋白信使核糖核酸的制备及其体外翻译

Preparation of human globin messenger RNA and translation in vitro

杂交捕获抑制聚腺苷二磷酸核糖聚合酶基因的体外翻译

Hybridization arrested translation in vitro of poly ( adp-ribose ) polymerase gene

在麦胚无细胞体外翻译体系中测定了它们的蛋白翻译活力。

Their translation activities were measured in a wheat germ cell-free system .

体外翻译证明该基因编码蛋白质分子量为55.3kD，与理论预测值一致。

The molecular weight of the protein is 55.3 kD identified by the translation in vitro .

新疆大麦条纹花叶病毒的研究&Ⅰ.病毒株系类型的鉴定及核酸的体外翻译

Study of barley stripe mosaic virus ( bsmv ) Xinjiang isolate ⅰ . identification and translation of viral RNA components

在无细胞体外翻译体系中能得到5～10倍于内源的同位素参入活力。

The translational activity of mRNA was found to be 5-10 times that of blank controls in wheat germ cell-free system .

经抗人白蛋白抗体免疫沉淀体外翻译产物，其参入活力约占总参入活力的3%。

After precipitation with anti-human albumin antibody , the amount of radioactivity in the precipitated portion was about 3 % of the total .

将体外翻译产物的免疫沉淀物经SDS-聚丙烯酰胺电泳，得到相当于人血清白蛋白的同位素标记的电泳区带。

When the immunoprecipitate was subjected to SDS agarose gel electrophoresis , 3 radioactive bands were obtained , one of which corresponded to pure human albumin in its position .

利用体外转录翻译系统和免疫共沉淀验证了这两种蛋白在体外的相互作用，结果表明KED样蛋白能够在体外与ACaMZ发生相互作用，并且这种作用是c扩+依赖型的。

We tested the interaction of these two proteins using in vitro transcription and translation system and coimmunoprecipitation . It was shown that the two proteins could interact in vitro and the interaction was Ca ~ ( 2 + ) dependent .

为了确定TM-TNF与细胞膜的关系，本文借助体外转录翻译系统及微粒体膜结构，建立了表达在微粒体膜上的TM-TNF以及无膜的裸26kDTNF。

In order to define the interrelation between TM-TNF and biomembrane , we used an in vitro translation system and microsomal membranes to engender expression models of two types of TM-TNF ; TM-TNF anchored to microsomes and naked 26kD TNF without membranes .

大鼠脑线粒体体外蛋白翻译体系的建立及蛋白产物的鉴定

Protien synthesis and characterization in isolated mitochondria from rat brain

乙型肝炎病毒前C变异体的体外转录和翻译

Study on in vitro transcription and translation of the precore variant of hepatitis B virus

HCVIRES特异性抑制性RNA结构模拟及在体外对病毒翻译启动的抑制作用

Structural modeling of HCV IRES specific inhibitor RNA and its inhibitory effect on viral translation in vitro