位点特异性重组
- 网络site-specific recombination;site specific recombination;Site-specifix recombination
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为彻底消除选择标记基因的负面影响,目前已经发展了多种去除选择标记基因的植物转化系统,如共转化系统、位点特异性重组系统、转座子系统及MAT载体系统等。
Presently many plant transformation systems have been developed in order to remove selectable marker genes such as co-transformation system , site-specific recombination system , transposable element system and MAT vector system etc.
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Cre/LoxP系统介导的位点特异性重组技术
Site-specific Recombination Technique Mediated by Cre / LoxP System
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位点特异性重组酶Cre的克隆、表达、纯化及体外活性鉴定
Cloning Expression Purification and Biological Activity of Site Specific Recombinase Cre
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位点特异性重组技术在繁殖普通核雄性不育系中的利用潜力
Molecular Strategies for Multiplication of Spontaneous and Artificial Genic Male Sterile Lines by Using Site - specific Recombination
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本文综述了共转化系统、位点特异性重组系统(包括FLP/FRT、Cre/lox、R/RS及Gin/gix系统)和转座子系统(Ac/Ds转座子系统)在培育无选择标记转基因植物中的应用。
The using of co-transformation system , site-specific recombination system ( FLP / FRT ? Cre / lox ? R / RS and Gin / gix systems ) and transposable element system ( Ac / Ds transposable element system ) in breeding marker-free transgenic plants were reviewed in this paper .