亚二倍体

产生梯状DNA并检测到亚二倍体峰（凋亡峰）；

Ladder DNA and aneuploid peak were detected .

流式细胞仪检测细胞出现亚二倍体峰，DNA电泳出现梯状带。

Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis .

流式细胞仪分析出现低于G1期DNA含量的亚二倍体凋亡率。

Sub-G1 cells were observed by flow cytometry , the total sub-G1-phase .

流式细胞仪分析发现在G1峰前出现一个亚二倍体峰即凋亡峰；

A apoptotic peak appeared on flow cyto metry ;

黄芪多糖处理后的BEL-7404细胞出现低于G1期DNA含量的亚二倍体凋亡峰，将细胞周期阻滞于G1期。

FCM analysis showed hypodiploid apoptosis peak after the liver cancer cells were treated with Astragalus polysaccharides .

FCM术检测在细胞周期G1期前出现亚二倍体凋亡峰；细胞周期分析显示G2/M期阻滞；

FCM results showed a typical sub-diploid peak before G0 / G1 phase and cell cycle analysis showed G2 / M phase arrest ;

p53-K562组和K562组、neo-K562组用TUNEL、AnnexinV和亚二倍体峰检测未发现有凋亡细胞比例差异。

P53 protein could not induce significant apoptosis compared to neo-K562 and wt-K562 with TUNEL , Annexin V and hypodiploid assays .

亚二倍体峰百分比在12、24h分别为37.10±7.21和43.72±6.73，均较对照组9.81±1.78显著增加（P＜0.01）；

The percentage of hypodiploid was 37.10 ± 7.21 and 43.72 ± 6.73 for 12 and 24 hours respectively , and both obviously increased compared with that of control groups 9.81 ± 1.78 ( P < 0.01 ) .

流式细胞仪检测发现在感染后18h出现G1-S相前出现凋亡染色体亚二倍体峰值，并且DNA电泳出现凋亡特征性的条带；

After 18-h infection , the apoptotic peak of hypodiploids appeared before G1-S phase and the bands with apoptotic characteristics were shown by DNA electrophoresis .

在流式细胞仪检测PI荧光直方图上，出现典型的亚二倍体峰，随着作用时间的延长，H4细胞凋亡率明显增加；

The peaks of sub-G1 appeared in histogram of FCM , which showed that the apoptosis rate of the H4 glioma cells increased significantly with the lapse of time .

方法：分别用AnnexinV-FITC和碘化丙啶（PI）染色，通过流式细胞仪测定AnnexinV阳性和出现亚二倍体DNA的凋亡细胞。

METHODS : Apoptosis was detected using annexin V-FITC and propidium iodide ( PI ) staining , respectively , and annexin V-FITC positive cells and hypodiploid cells were analyzed by flow cytometry .

流式细胞仪分析可见亚二倍体（Sub-G1）凋亡峰；试论现代侦查模式的构建

The apoptosis peak was showed by FCA detection . The Discussion on Constitution of Current Detection Mode

CAI对MCF-7细胞的凋亡诱导作用通过两种方法测定：应用流式细胞仪分析细胞的亚二倍体凋亡峰以及使用AnnexinV-FITC／PI凋亡检测试剂盒定量分析磷脂酰丝氨酸外翻。

Apoptosis induction by CAI in MCF-7 cells was assessed by ( I ) flow cytometry analysis of sub-G_0-G_1 DNA content and ( ii ) quantification of phosphatidylserine exposure using annexin V-FITC kit .

流式细胞术检测示细胞阻滞于G0/G1期，并且出现典型的亚二倍体（Sub-G1）峰；

Flow cytometry showed that the cells were blocked at G_0 / G_1 phase and typical Sub-G_1 peak appeared .

TUNEL指数分别为0.387和0.613，亚二倍体凋亡峰比例分别为3.8%和10.7%，前者均明显低于后者（P<0.05）。

Their TUNEL index was 0.387 and 0.613 respectively , and their sub-G1 apoptotic peak scale was 3.8 % and 10.7 % respectively , the former were all lower than the latter ( P < 0.05 ) .

与对照组（7±1）%相比，24hG2/M期细胞比例亦减少，为（2±2）%，但OCT未改变亚二倍体细胞的比例。

At 24h , the percentage of G2 / M cells also decreased to 2 ± 2 % as compared with the controls ( 7 ± 1 % ) . OCT did not change the percentage of hypodiploid cells .

200～300μg／ml的EGCG处理MGC-803细胞和BEL-7402细胞24～36h，在琼脂糖凝胶电泳上可见凋亡细胞特征性的梯状带和PI染色流式细胞仪直方图上可见亚二倍体峰；

Treatment of MGC-803 cells and BEL-7402 cells with 200 ~ 300 μ g / ml EGCG for 24 ~ 36 h resulted in apoptosis-associated alterations : " ladder " bands on agarose gel electrophoresis and hypodiploid peaks on flow cytometric histogram .

6%乙醇可使HCC9204细胞产生明显的凋亡形态学变化，大部分细胞为TUNEL阳性，并且流式细胞仪分析可见明显的亚二倍体凋亡峰。

6 % ethanol could lead to obviously morphological apoptotic changes in HCC 9204 cells , most of the cells were TUNEL positive , and obviously apoptotic sub G1 peak was found by flow cytometry .

经钩吻醇提水沉组分处理的HL60细胞周期状态发生明显变化，表现为G0G1期细胞比例降低，S期细胞比例增加，并出现较明显的亚二倍体细胞峰型。

The cells treated with the components of Gelsemium by ethanol extracting and water deposition appeared obviously cell cycle state changes , ratio of G0 / G1 phase increased and S phase decreased , a distinct sub-diploid peak can be seen .

对细胞周期分析表明，随着小檗碱浓度的增加，处于亚二倍体峰的细胞数增加，处于S和G2M期的细胞数则减少，而G0G1期的细胞数基本不变。

Flow cytometry analysis of the cell-cycle distribution revealed that the cell number in sub-G 0 / G 1 peak ( apoptotic peak ) was increased , while the cell numbers in S and G 2 / M was decreased as the concentration of Ber increased .

流式细胞术分析可见明显的亚二倍体凋亡峰。

Typical apoptotic sub G1 peak was observed by flow cytometer .

亚二倍体嵌合体母猪及其子代染色体组型分析

Chromosome complement of a sow with mosaic sub-diploid and her daughters

报告了1头亚二倍体嵌合体的母猪（403号）。

A sow with mosaic sub diploid was reported .

最常见的染色体数目改变是超二倍体，其次是三倍体，四倍体和亚二倍体。

The most common numerical alteration was hyperdiploidy .

流式细胞仪检测出现典型的亚二倍体凋亡峰。

Atypical subdiploid peak was observed by flow cytometry . The apoptosis peak was showed by FCA detection .

流式细胞仪检测显示出大量亚二倍体细胞核型峰（凋亡峰），二倍体细胞峰减少。

Flow cytometer detection : Lots of sub-diploid nuclear peak appeared ( apoptotic peak ) and diploid cells peak reduced .

流式细胞仪检测到约9.6×105细胞出现亚二倍体峰，二倍体峰几近消失；

Sub diploid peak which contained 9 6 × 10 cells was detected by flow cytometry and diploid peak almost disappeared .

对照组未见到明显的亚二倍体峰，各实验组均检测到典型的亚二倍体峰。

The control group did not see obvious the diploid peak , each group are detected the typical the diploid peak . 4 .

流式直方图上可看到在正常二倍体峰（Go/G1）前出现典型的亚二倍体凋亡峰。

Typical hypodiploid apoptotic peak were observed before the diploid peak ( Go / G1 ) appearing in flow histogram in the detection of flow cytometry .

结果ALL异倍体检出率为514%（1835），其中亚二倍体8例，超二倍体10例；

Results The aberrant DNA aneuploid was found in 51 4 % of the ALL patients examined ( 18 / 35 ), which included 8 hypodiploid and 10 hyperdiploid .