vhb

The biological activity of the expressed VHb was demonstrated by CO difference spectrum analysis .

经CO差示光谱分析测定，该蛋白具有生物活性。

The VHb activity in recombinant was detected by CO difference spectrophotometry .

通过一氧化碳（CO）差光谱检测到了VHb的表达。

PCR analysis indicated vhb gene has been integrated into chromosome of Streptomyces fradiae .

PCR验证表明，vhb基因已整合到染色体上，CO结合差光谱分析则表明vhb基因在弗氏链霉菌中成功表达。

Cloning and expression of VHb gene in d-arabitol producing yeast

细菌血红蛋白基因在产D-阿拉伯糖醇酵母菌中的克隆与表达

We use the carbon monoxide differential spectrometry to determine the activity of VHb which is expressed by recombinant strain .

利用CO差光谱法对重组菌表达的透明颤菌血红蛋白进行了活性及定量检测。

The 16 kD VHb protein was detected after hot-shock inducing expression of the engineered E. coli .

BL21并经热激诱导表达后，在培养物中检测到了约16kD的VHb蛋白。

The gene of VHb was cloned in 6-81 cell ; it has higher rate of PTA degradation in the lower deliquescence oxygen environment .

通过将VHb基因克隆入对PTA具有降解功能的受体菌中，可使其在较低的溶解氧环境中，仍具有较高的降解率。对VHb在污水处理系统中的应用作了初步的探索。

An improved carbon monoxide ( CO ) differential spectrometry assay method was established for the rapid determination of Vitreoscilla hemoglobin ( VHb ) expression .

为快速、准确检测重组菌中透明颤菌血红蛋白（VHb）的活性表达，本文改进了CO差光谱法。

The ability of Vitreoscilla hemoglobin ( VHb ) to capture and release oxygen can better meet the needs of oxygen in the fermentation of host cells .

透明颤菌血红蛋白所具备的捕捉和释放氧气的能力使之在宿主细胞发酵时可以更好地满足宿主细胞氧气的需求。

Under the same fermentation condition , the VHb expression strain and its control strain absorbed 3.1 % and 2.2 % applied oxygen , respectively . VHb expression strain uptake oxygen 1.41 fold than its control stain .

在相同发酵条件下，VHb表达菌株和对照菌株分别摄取了通入氧气量的3.1%和2.2‰VHb的表达菌株比对照菌株多利用了41%的氧气。

In the LC50 bioassay on Helicoverpa armigera of high and low volume flask culture , the LC50 of VHb expression strain reduced 36.6 % and 10.0 % , respectively . 3 .

高、低两种装液量摇瓶发酵中，VHb表达菌株与对照相比，其对棉铃虫（Helicoverpaarmigera）幼虫的LC50值分别下降了36.6%和10.0%。

The purpose of this study is to express VHb gene ( vgb ) in Bt , and to improve cell growth and insecticidal crystal proteins ( ICP ) production by enhancing the oxygen usage ability of cell . 1 .

本研究将透明颤菌血红蛋白基因（vgb）表达于Bt中，通过提高细胞对氧气的利用能力，达到促进菌体生长和产物合成的目的。

It revealed that the integral VHb and acetate metabolism pathway ( Pta Ack ) block improved the growth of host strain under low dissolved oxygen conditions , enhanced the recombinant proteins production and reduced the accumulation of acetate harmful to bacterial growth .

整合型vhb的表达及乙酸代谢途径（PtaAck）的缺陷，改善了宿主在贫氧条件下的生长，且促进了重组蛋白的表达。

Cases with severe viral hepatitis B ( VHB ) were treated with hepatic growth factor . The death rate in this patient group was 37 5 % while the death rate in the control group which were not applied to hepatic growth factor was 65 0 % .

应用肝生素治疗各种重型乙型病毒性肝炎。结果表明，40例重型乙型病毒肝炎患者经肝生素治疗，治疗组死亡15例，病死率375%；

An improved method using the carbon monoxide ( CO ) difference spectrum was used to analyze Vitreoscilla hemoglobin ( VHb ) in recombinant Escherichia coli , VG1 ( pTU14 ), producing poly - β - hydroxybutyrate ( PHB ) .

为检测产聚β-羟基丁酸酯（PHB）的重组大肠杆菌VG1（pTU14）中表达的透明颤菌血红蛋白（VHb），建立了改进的CO差光谱法。

We construct the plasmid pUC-LV by adding synthesized natural promoter of the vgb gene into the vgb gene . After induction under hypoxic conditions , the density of recombinants increases 157 % more than those of original stain and the VHb protein with the physiological activity is expressed .

合成vgb基因的天然启动子，与vgb结构基因拼接，构建重组质粒pUC-LV，通过诱导表达，重组子的菌密度增加了157%，得到了具有生理活性的15.7kD的VHb蛋白。