puc

Conclusion : PUC has a good protective effect on hepatic injury .

结论：叶下珠复方有很好的保护肝细胞作用。

A method of efficiently cloning of PCR products using PUC plasmid

一种用PUC质粒高效克隆PCR产物的方法

The study and application of PUC waterproof and anticorrosion material

新型PUC聚氨酯弹性防水、防腐涂膜材料的研制及应用

Implementation Technique of the PUC V Card Outline Chinese Font

方正V型卡曲线汉字实现技术

This paper generalizes the method of analysing the PUC V Card .

本文概述了分析方正V型卡曲线汉字技术的方法。

Effect of Lanthanum (ⅲ) on Replication Copy Number of Plasmid pUC 18

La（Ⅲ）对质粒pUC18复制拷贝数的影响

Construction of Two Highly Efficient pUC 18 T-vectors

两种pUC18高效T载体的构建

Study on FT-Raman Spectroscopy of pUC 18 DNA

质粒pUC18DNA的傅立叶变换拉曼光谱研究

A new method , simple but of high performance , for the ligation of pCR blunt product with pUC Vector was presented here .

介绍一种使用pUC质粒做为载体进行平端PCR产物连接的方法。

Molecular cloning by PUC 18 T vector and nucleotide sequence of the RT PCR product of classical swine fever virus

猪瘟病毒RT-PCR产物的T载体克隆、测序及同源比较

These double strands were cloned into a plamid PUC 12.The successful cloning was confirmed by site hybrid screening and DNA sequencing .

再把这两个双链DNA克隆到PUC12质粒中。经点杂交筛选和DNA顺序测定证明克隆是成功的。

Insetting of 35S promoter into the plasmid vector PUC 13-pb1 of the cyanobacteria

35S启动子组入蓝藻质粒载体pUC13－pbl

Recombinant PUC inserted with full length nucleic acid sequence of chrysanthemum stunt viroid was used as template DNA to prepare digoxigenin-labelled and biotin-labelled probes with two universal primers by polymerase chain reaction .

以含菊花矮化类病毒（Chrysanthemumstuntviroid.CSV）全长序列的重组质粒PUC为模板DNA，加入通用引物，在聚合酶链式反应系统中制备地高辛标记的CSVcDNA探针和生物素标记的CSVcDNA探针。

And postal equipment management and use of PUC from the ground level is divided into three systems are : cities , counties , outlets , maintenance and management personnel at all levels of the use of different functions and powers .

并且邮政设备管理使用从地市局层面分为三级体系为：市、县、网点，各级使用维护管理人员职责权限不同。

Methods The mRNA from normal lungs was used as template . By using the techniques of RT PCR , nest PCR and cloning , the pUC 19 / SP A 6 A clone was established and sequenced .

方法以正常人肺组织mRNA为模板，采用逆转录反应（RT）PCR、巢式PCR及基因克隆技术，获得pUC19/SPA6A克隆，并测序证实。

The protective actions of Tea Polyphenols ( TP ) on free radical damage were determined by using hemolysis test , assay of MDA level in mice tissue homogenates and the change of conformation of plasmid PUC 18 irradiated .

采用溶血试验，小鼠肝、肾组织脂质过氧化产物丙二醛（MDA）测定，受照质粒构象改变等方法测定茶多酚对生物大分子物质氧自由基损伤的保护作用。

Methods : A cDNA fragment from the mRNAs of skeletal muscle of 13 day old chicken embryo was amplified by RT PCR method , and inserted into pGEM and pUC vectors at clone sites and sequenced respectively .

方法：从13d鸡胚骨骼肌mRNAs经RTPCR方法扩增RTEF1的编码序列（cDNA），将其克隆到pGEM和pUC载体并进行核苷酸序列分析。

Methods PCR product of vp4 gene was directly cloned into pUC T vector , and the vp4 DNA fragments were cut down by restriction enzymes and inserted into the plant expression vector . The recombinant plasmids were transformed into Agrobacterium tumefaciens by electroporation .

方法抗原基因vp4经PCR扩增后直接克隆到pUCT载体，双酶切目的片断和植物表达载体，以T4连接酶将目的片断与载体相连接，电转化方式将重组质粒转入农杆菌。