Using [ 3H ] PIP2 as the substrate , the PLC activities in the culture supernatant , and cytoplasma and cytomembrane of the three strains of Leptospira were measured by isotope assay .
以[3H]PIP2为底物，采用同位素法检测上述3株钩体培养物上清、胞浆和胞膜中PLC的活性。
pip2