imcd

Effect of respiratory acid-base unbalance on carbonic anhydrase activity and its mRNA expression in primary cultured rabbit renal IMCD cells

呼吸性酸碱失衡对家兔肾脏内髓集合管细胞碳酸酐酶活性及其mRNA表达的影响

Experimental study on h ~ + / k ~ + exchange function and ch-k-atpase mRNA expression in renal IMCD cell monolayer under respiratory acid-base disorders in rabbit

呼吸性酸碱失衡家兔肾脏内髓集合管细胞单层H~+/K~+交换功能及cH-K-ATP酶mRNA表达的实验研究

Preparation for a primary cultured model of IMCD cells under respiratory acid-base disorders

呼吸性酸碱失衡内髓集合管细胞培养模型的制备

Methods CA activity in unbalanced IMCD cells was measured with imidazole Tris technique .

方法采用咪唑Tris技术测定呼吸性酸碱失衡IMCD细胞的CA活性；

Conclosion : The primary cultured cell in this study , is IMCD cell of rabbit kidney .

结论：本文培养的细胞为家兔肾脏IMCD细胞。

Conclusion : IMCD cells over-expressing PPAR γ have anti-inflammatory and anti-fibrosis effects .

结论：IMCD细胞转染PPARγ野生型质粒使得PPARγ高表达后具有抗炎和抗纤维化作用。

METHODS To measure H + / K + exchange in the primary cultured IMCD cell monolayers under different CO2 concentrations by the BCECF / AM fluorescent probe .

方法采用BCECF／AM荧光探针法，测定不同浓度CO2培养的IMCD细胞H＋／K＋交换。

Objective To evaluate the effect of furosemide on Cl - / HCO - 3 exchange of inner medullary collecting duct ( IMCD ) in rabbit kidney .

目的评价速尿对家兔肾脏内髓集合管（IMCD）Cl-/HCO-3交换功能的影响。

Objective To study the compensatory regulation of the Cl - / HCQ3 - exchange of kidney inner medullary collecting duct ( IMCD ) cell under respiratory acid-base disturbance conditions .

目的研究肾脏内髓集合管（IMCD）细胞Cl~-/HCO3~交换在呼吸性酸碱失衡时的代偿调节作用。

The main abnormalities were plugging of inner medullary collecting ducts ( IMCD ) and Bellini ducts ( BD ) with deposits of calcium phosphate in the form of apatite ;

主要的异常是磷酸钙以磷灰石的形式沉积在内髓集合管和Bellini管，造成其堵塞；

Objective To investigate the effects of respiratory acid base unbalance on carbonic anhydrase ( CA ) activity and its type ⅳ mRNA expression in primary cultured rabbit renal inner medullary collecting duct ( IMCD ) cells .

目的探讨呼吸性酸碱失衡对家兔内髓集合管（IMCD）细胞碳酸酐酶（CA）活性及其mRNA表达的影响。

Objective To investigate the effects of omeprazole on H + / K + exchange of inner medullary collecting duct ( IMCD ) cells was inhibited by omeprazole and whether the effects could be abolished by washing out .

目的探讨奥美拉唑对家兔肾脏IMCD细胞H+K+交换的影响，以及经洗涤处理是否解除这种影响。

Conclusion ADR stimulated Na + influx into IMCD cells cultured in vitro , and the stimulative effect is not regulated by other effecting factors . The events observed in vitro in the study might also be in the case of Na + retention in nephrotic state in vivo .

结论ADR刺激Na＋经管腔膜面的入胞作用，且这一作用不受上述诸效应物质的影响，这一体外研究结果可能适用于肾病时钠潴留的体内情况。