NT3

Adenovirus-mediated NT3 gene transfer protects spiral ganglion neurons from degeneration after noise trauma

腺病毒介导的NT3基因转染对噪音损伤的耳蜗螺旋神经节细胞的保护作用

We are to use this scaffold and the recombinant NT3 to save SCI .

本课题拟利用这一生物支架与重组NT3联合治疗脊髓损伤，观察其治疗效果。

Protective Effects of Adenoviral-mediated NT3 Gene on Spiral Ganglion

NT3重组腺病毒对耳蜗螺旋神经节的保护作用

With the combination of PLGA and NT3 treatment , there was partial functional recovery in the injured rats .

经过PLGA和NT3的联合治疗，损伤后的大鼠运动功能部分恢复。

Human neurotrophin-3 ( NT3 ) gene has 774 bp , which codes 257 amino acid residues .

神经营养素3（neurotrophin-3，NT3）基因的大小为774bp，编码257个氨基酸残基。

Cloning and identification of normal mouse NT3 gene and the establishment and screening of cell strain expressing high level of NT3 gene

NT3基因的克隆、鉴定及高表达胚胎干细胞的构建与筛选

To construct and transform yeast bait plasmid carrying neurotrophin-3 ( NT3 ) cDNA fragment .

构建和转化神经营养素3（neurotrophin3，NT3）的酵母诱饵重组质粒，为通过酵母双杂交研究NT3的功能及作用机制奠定基础。

Basic and Prepared Experiments of Neurotrophins ( BDNF , NT3 ) Gene Therapy to Neurosensory Deafness or Hearing Loss

神经营养因子（BDNF，NT3）基因转染治疗和/或保护感音神经性聋的前期基础及预备实验研究

Four weeks after the inoculation of the virus , NT3 immunoreactivity was observed in the Ad-NT3 inoculated group .

4周后，经NT3抗体免疫细胞化学染色可见，在注入AdNT3病毒的实验组中，在内耳多种细胞中有明显的NT3蛋白的表达。

Since Windows NT3 x , 4 0 operating system has no disk-quota capability , it can not control effectively the use of disk space of server .

WINDOWSnt3x，40操作系统无磁盘配额功能，无法有效控制服务器磁盘空间的使用。

Results After G418 sifting , astrocytes modified by gene transfected by NGF , BDNF , and NT3 gene showed G418 positive ;

结果：经G418筛选后，转染NGF、BDNF和NT3基因修饰星形胶质细胞呈G418抗性；

Rabbit anti-rat NT3 polyclonal antibody and rabbit anti-rat BDNF polyclonal antibody were bought from Boster Company .

兔抗大鼠神经营养素-3多克隆抗体和兔抗大鼠BDNF多克隆抗体（博士德公司）；

Collagen material could serve as a physical bridge to connect the rostral and caudal of the injured spinal cord and a vehicle to deliver NT3 .

胶原材料可以作为连接损伤脊髓头端和尾端的物理桥梁，也可以成为运输NT3的载体。

Objective To observe the influence of neurotrophin-3 ( NT3 ) transfection in vitro on the growth of mice cochlear spiral ganglion cells ( SGCs ) .

目的通过神经营养素3（NT3）基因转染的方法，了解NT3对耳蜗螺旋神经节细胞（SGC）生长的影响。

The effect of delaying denervated muscle atrophy will increase significantly after the neural stem cell induced to motor neuron in vitro or in the mean time transfected neurotrophic factor NT3 .

神经干细胞经过体外向运动神经元诱导或同时转染神经营养因子NT3后，其延缓肌肉萎缩的效果明显增强。

Objective To observe the expression of Adenoviral-mediated neurotrophin-3 ( NT3 ) in the cochlea of guinea pig , and evaluate the activity of NT3 in spiral ganglion cell following noise damage .

目的观察含有神经营养素-3（neurotrophin-3，NT3）的重组腺病毒在豚鼠耳蜗中的表达及其在噪声损伤后对螺旋神经节的保护作用。

After induced in vitro , the proportion of neurons differentiated from GFP-NSCs was obviously increased and the differential time was advanced . The ratio of neurons differentiated from GFP-NSCs transfected NT3 gene was raised further .

经体外诱导的神经干细胞体内移植后向神经元分化的比例明显提高而且时间提前；经体外诱导并转染NT3的神经干细胞分化为神经元的比例进一步提高。

Conclusion In diabetic rats , the sciatic nerve shows a longer latent period , a slower conduction velocity , a lowered pain threshold and diminished expression of NT3 and NF in the motor neurons in the anterior horn .

结论糖尿病大鼠坐骨神经的传导速度减慢，潜伏期延长，痛阈降低，其脊髓前角区运动神经元NT3与NF的表达明显减少。

After two rounds of CsCl centrifugation , the human recombinant adenovirus Ad BDNF and Ad NT3 were obtained with titer of 1 × 10 12 and 8 × 10 11 pfu / ml , respectively .

经PCR鉴定后，CsCl超离心纯化获得了滴度分别为1×1012和8×1011的BDNF和NT3重组腺病毒。

AIM : To construct Tetracycline ( tet ) inducible recombinant adenovirus vectors containing human neurotrophin 3 ( NT3 ) and brain derived neurotrophic factor ( BDNF ) genes , respectively and perform PCR and restrictive enzyme digestion analysis .

目的：分别构建含人神经营养素3（NT3）基因和脑源性神经营养因子（BDNF）基因的重组四环素可诱导的腺病毒载体，并进行PCR和酶切鉴定。

Numerous studies have shown that the health of spiral ganglion neurons is highly important for hearing . As a trophic factor of spiral ganglion neurons , neurotrophin 3 ( NT3 ) is a potential candidate for prevention of spiral ganglion neuron degeneration in human .

神经营养素3（neurotrophin3，NT3）作为螺旋神经节细胞特异的营养因子，可有效地支持内耳传入神经元的存活，因此有望成为治疗因其退变而引起的感音性神经性耳聋的有效因子。

Furthermore , these regenerating axons consisted with the functional recovery , indicated that the regenerating neural fibers could promote locomotor functional recovery . Conclusions : The system of the ordered collagen scaffold and collagen-targeting NT3 could effectively deliver NT3 to treat spinal cord injury .

结论：有序胶原支架和胶原靶向NT3能构成有机的体系，从而有效运载NT3，更有效的发挥NT3治疗脊髓损伤的作用。该治疗体系能更有效地促进运动功能的恢复和神经纤维的再生。

To study the trophic effects of brain-derived neurotrophic factor ( BDNF ) and neurotrophin-3 ( NT3 ) on the SGNs and the potential usage of gene therapy in the auditory neuron injury , the dissociated SGNs from 5 day postpartum ( P5 ) rats were cultured .

为了观察脑源性神经营养因子（BDNF）和神经营养素-3（NT3）对听神经元的营养作用，并研究基因治疗在听神经损伤治疗中的可行性。