EMSA

The promoter regions of these genes were PCR amplified , and then EMSA was carried out to study the binding of the purified AlmR ⅰ protein to these regions .

利用PCR扩增得到了这些基因的启动子区，然后通过凝胶阻滞实验对纯化的AlmRⅠ蛋白与所扩增片段的结合进行了研究。

The Detection of Triplex DNA in EMSA Analysis by Silver Staining

银染法用于EMSA分析检测三链核酸形成

The Essential Conditions of Effectively Implementing EMSA

对组织有效实施EMSA的基本条件

A New Method for EMSA by Modifying DIG High Prime DNA Labeling and Detection Starter Kit ⅱ

改造地高辛标记DNA和检测试剂盒用于凝胶阻滞实验的新方法

Analysis of sequence-specific DNA-binding protein of human α 1 (ⅰ) procollagen gene with improved EMSA

改良EMSA法分析人α1（Ⅰ）胶原基因序列特异性DNA结合蛋白

Then , we verify this conclusion again with the ChIP and EMSA assays .

随后，我们使用ChIP实验和EMSA实验验证了此结论。

EMSA analyses of transcription factors , C / EBP and HMG-like protein in human lung cancer cells

人肺癌细胞转录因子&C/EBP和HMG样蛋白的EMSA检测

NF κ B DNA binding activity was evaluated with electrophoretic mobility shift assay ( EMSA ) .

采用：（1）凝胶电泳迁移率改变分析检测核因子（NF）κbDNA结合活性；

Determination of HIF-1 α Binding Activity by EMSA in Pulmonary Arteries of Rats With Hypoxia-induced Pulmonary Hypertension

HIF-1α结合活性及蛋白含量的表达变化在缺氧性肺动脉高压大鼠中的作用和意义

The E2F1 DNA binding activities were measured by gel mobility shift assay ( EMSA ) .

用凝胶迁移率变动分析法（EMSA）检测其与DNA的结合活性。

Methods Electrophoretic mobility shift assay ( EMSA ) was employed to determine STAT-DNA binding activity .

方法采用凝胶阻滞电泳（EMSA）测定DNA-STAT结合活性变化。

Electrophoretic mobility shift assay ( EMSA ) was used to detect the NF - κ B / DNA binding activity .

用凝胶电泳迁移率改变试验（EMSA）检测肺动脉NFκB/DNA结合活性。

The DNA biding activity of AP-1 was analyzed by electrophoretic gel mobility shift assay ( EMSA ) .

电泳迁移率变更分析（EMSA）检测AP1DNA结合活性的变化；

Transcription factor c-myb activity was confirmed by electrophoretic mobility shift assays ( EMSA ) in the two cell lines .

用凝胶电泳迁移率变动分析（EMSA）验证有活性差异的转录因子c-myb。

Meanwhile , pulmonary STAT-1 DNA-binding activity was detected by electrophoretic mobility shift assay ( EMSA ) .

凝胶电泳阻滞分析（EMSA）测定STAT-1的DNA结合活性；

DNA binding activity of the Egr-1 protein in liver nuclear extracts was determined by electrophoretic mobility shift assay ( EMSA ) .

肝核提取物中Egr-1蛋白与DNA的结合活性；

First , we carried out Electrophoretic Mobility Shift Assay ( EMSA ) to investigate the binding difference of SNPs and transcription factors .

首先使用凝胶迁移检测和疾病关联的SNP是否会影响某些转录因子的结合。

Activation of activator protein-1 ( AP-1 ) was measured by electrophoretic mobility shift assay ( EMSA ) .

电泳迁移率变动分析法（EMSA）研究核转录因子SP-1的活性。

The NF - κ B binding activity was analyzed by electrophoretic mobility shift assay ( EMSA ) after 1-hour incubation .

孵育1h，用电泳迁移率检测核因子κB相对活性。

The biological activity of renatured AIF protein was detected by electrophoretic mobility shift assay ( EMSA ) and Hoechst staining .

采用凝胶滞后实验（EMSA）与Hoechst33258染色检测AIF蛋白的生物学活性。

The method for detection of NF - κ B-DNA binding activity in the aorta of three groups was electrophoretic mobility shift assay ( EMSA ) .

实验结束时，用电泳移动迁移技术（EMSA）检测三组兔主动脉组织中NF-κB-DNA结合活性；

The antigen-binding activity of the ScFv / tP fusion protein was confirmed by indirect ELISA , and the DNA binding ability was confirmed by EMSA .

间接ELISA分析ScFv/tP融合蛋白的亲和活性，凝胶迁移阻滞实验检测ScFv/tP融合蛋白与DNA结合的情况。

TESTING The activation of NF-AT in T Lymphocyte of EAU was assayed by using electrophoretic mobility shift assay ( EMSA ) .

采用电泳迁移率改变试验对EAUT淋巴细胞NF-AT活性检测。

Relation standards of chemical component , microstructure , application performance , cost and safety parameters of EMSA are achieved or exceeded by using of total production maintenance ( TPM ) and value project .

通过TPM系统的推行和价值工程的应用，使镁合金挤压阳极的化学成分、微观组织，使用性能、成本以及安全指标达到了或超过了有关标准的要求。

DNA binding proteins that bind specifically to three probes of positive footprinting area of CEA promoter were detected by electrophoretic mobility shift assay ( EMSA ) .

利用电泳迁移分析法（EMSA）检测这些细胞株与CEA启动子阳性足迹区探针结合的DNA结合蛋白。

Transcriptional and DNA binding activity were analyzed by reporter gene assay and electrophoretic mobility shift assay ( EMSA ) respectively . Western blot was used to determine the protein expression ;

运用报告基因法和凝胶电泳迁移率法（EMSA）分析AP1和NFκB反式激活活性和DNA结合活性，蛋白质印迹检测蛋白质表达；

Furthermore , Western blot and gel electrophoresis mobility shift assay ( EMSA ) were performed to detect the mechanism of PLC γ 1 in colorectal cancer cell adhesion to matrix .

PLCγ1在结直肠癌细胞中作用的信号转导机制采用蛋白质印迹及凝胶迁移率变动分析（EMSA）。

Western blot was used to detect the protein levels of Par-4.Electrophoretic mobility shift assay ( EMSA ) was applied for evaluating the change of STAT_3 DNA binding activity .

westernblot测定Par-4的蛋白表达量，凝胶迁移改变实验测定STAT3的DNA结合力。

Methods The inhibitory effect of circular dumbbell decoy ODNs on the transcriptional activity of NF κ B was detected by electrophoretic mobility shift assay ( EMSA ) .

方法采用电泳迁移率改变实验（EMSA）体外检测哑铃形圈套ODNs对NFκB转录活性的抑制效应。

Methods The APRF activity of DNA-binding protein in PBMC of 40 patients with SLE and 20 normal controls was detected by electrophoresis mobility shift assays ( EMSA ) .

方法采用凝胶阻滞电泳（EMSA）的方法检测40例SLE患者及20例正常对照组PBMC中DNA结合蛋白APRF的表达水平。