DHPLC

At present , denaturing high-performance liquid chromatography ( DHPLC ) is the best technique platform for detecting mutation .

变性高效液相色谱（DHPLC）是目前检测基因突变的最佳技术平台之一。

Objective Of denaturing high performance liquid chromatography ( DHPLC ), a technique platform was developed for screening G6PD deficient variants .

目的建立变性高效液相色谱筛查G6PD基因变异的技术。

TNF-d genotype was analyzed by DHPLC ( denature high performance liquid chromatography ) and DNA sequencing .

获取受者及其同胞供者的DNA，通过变性高效液相色谱（DHPLC）法结合DNA测序检测TNF-d基因型。

SNPs were screened by denatured high performance liquid chromatography ( DHPLC ) techniques and restriction fragment length polymorphisms .

用变性高效液相色谱和限制性酶切片段长度多态检测CHRNA4的多态位点，测序确定变异的碱基，统计各多态位点基因频率。

Methods The identification of SNPs was performed by both direct DNA sequencing and denaturing high-performance liquid chromatography ( DHPLC ) .

方法用直接测序法和变性高效液相色谱法（DHPLC）进行SNP检测，用直接测序法对EH组和正常血压组（NT）进行SNP基因分型。

Methods The polymorphism of MTHFR gene in 87 IS patients and 83 controls was analyzed by denaturing high performance liquid chromatography ( DHPLC ) .

方法运用变性高效液相色谱（DHPLC）技术检测87例海南黎族IS组及83例对照组的MTHFR基因多态性。

Methods G6PD mutations were identified by combination of denaturing high performance liquid chromatography ( DHPLC ), DNA sequencing and restriction endonuclease assay .

方法对广西30例G6PD缺乏症的基因型使用变性高效液相（DHPLC）筛选，然后使用直接测序或限制性内切酶进行再检测。

Objective : To observe the value of detecting mutation in coagulation factor XIIIA chain gene by denatured high performance liquid chromatography ( DHPLC ) .

目的：观察变性高效液相色谱（DHPLC）法检测凝血因子XIIIA链基因突变的实用价值。

Objective To establish and evaluate denaturing high-performance liquid chromatography ( DHPLC ) as a rapid and efficient technique of detecting mutations of mitochondrion gene .

目的以变性高效液相色谱（DHPLC）技术分析检测线粒体肌病患者线粒体基因突变，以明确诊断。

CONCLUSION : We first amplified mtDNA in circulating plasma from breast cancer patients by direct PCR , checked mutation by DHPLC and confirmed results by sequencing .

结论：本实验直接用PCR扩增乳腺癌血浆核酸中线粒体基因，并用DHPLC初步筛选突变并测序证实。

Microdissection , PCR , and denaturation high performance liquid chromatography ( DHPLC ), and DNA sequencing were used to detect the mutation of p53 gene .

显微切割技术分离微量肿瘤组织制备DNA进行聚合酶链反应扩增；变性高效液相色谱技术（DHPLC）和DNA测序技术检测内源性p53基因突变的分布和类型。

Objective : To explore the effectiveness of denaturing high-performance liquid chromatography ( DHPLC ) by detecting the mutation in cancer tissues and serums of patients with gastric cancer .

目的：探讨部分变性高效液相色谱（DHPLC）在检测胃癌组织、血浆中基因突变中的应用。

DJ-1 mutations were detected by using Polymerase chain reaction ( PCR ) and denaturing high performance liquid chromatography ( dHPLC ) in early-onset PD and family Parkinsonism .

采用聚合酶链反应、变性高效液相色谱（dHPLC）及DNA测序等技术，对四川地区汉族人群中早发性帕金森病及家族性帕金森综合征患者DJ-1基因进行突变筛查。

Denatured high performance liquid chromatography ( DHPLC ) was used to array the long terminal repeat DNA fragments , and then the nucleotide sequence of LTR was analyzed by automatic sequence meter .

变性高效液相分析和序列测定LTR片段核苷酸序列，对不同株基因序列作同源性的比较分析。

Denaturing high performance liquid chromatography ( DHPLC ) is a novel high-throughput technique for screening DNA variations . It can be highly sensitive to detect mutations , SNPs , LOH and make genotype analysis .

变性高效液相色谱分析（DHPLC）是近年来发展起来的高通量筛选DNA结构变异的一项新技术，可以高灵敏地检测突变、SNP、LOH，以及进行基因型分析等。

Objective To compare the effectiveness of using multiple ligation probe amplification ( MLPA ) and denaturing high-performance liquid chromatography ( DHPLC ) in screening the exon deletions and duplications of the DMD gene .

目的比较多重连接依赖式探针扩增法（MLPA）和变性高效液相色谱法（DHPLC）检测Duchenne型肌营养不良症（DMD）患者DMD基因缺失/重复突变的效果。

Polymerase chain reaction ( PCR ), denaturing high performance liquid chromatography ( DHPLC ), and sequence analysis were used to screen the single nucleotide polymorphisms and the genotype distribution of - 675 4G / 5G located in the promoter region of the PAI-1 gene .

联合应用聚合酶链反应（PCR）、变性高效液相色谱分析（DHPLC）和DNA直接测序分析PAI-1基因启动子区域的4G/5G多态性。

Objective To explore the use of denaturing high-performance liquid chromatography ( DHPLC ) in detecting single-nucleotide polymorphisms ( SNPs ) of insulin receptor substrate-2 ( IRS-2 ) gene 3 ′ - untranslated region ( 3 ′ - UTR ) .

目的：探讨变性高效液相色谱（DHPLC）在胰岛素受体底物2（IRS2）基因3′非翻译区单核苷酸多态性（SNP）检测中的应用。

Methods Genomic DNA was extracted from the peripheral blood lymphocytes of 22 patients with clinically diagnosed FAP and was forwarded to screening for germline mutations by using denaturing high-performance liquid chromatography ( DHPLC ), protein truncation test ( PTT ) and DNA sequencing in APC gene .

方法从22例临床确诊的FAP患者，外周静脉血中提取基因组DNA。变性高效液相色谱、蛋白截短检测、测序技术结合应用进行全基因分析。

PCR was performed with AmpliTaq Gold DNA polymerase and 100 ng genomic DNA to amplify the entire coding sequence and exon-intron boundaries of c-kit exon 17 ~ exon 19 . Denaturing high-performance liquid chromatography ( DHPLC ) analysis was performed on a WAVE DNA Fragment Analysis System .

应用PCR测定、变性高效液相色谱技术及DNA测序方法对患者进行c-kit基因外显子17~外显子19突变检测。