猪囊尾蚴

猪囊尾蚴抗原-B基因cDNA编码区的克隆

Cloning of Cysticercus cellulosae AGB cDNA coding region

猪囊尾蚴cDNA表达文库的构建、筛选及目的基因的克隆和鉴定

The Construction Screening of cDNA Expression Library of Cysticercus Cellulosae and Cloning Identification of Interested Genes

本文就猪囊尾蚴病DNA疫苗的研究现状作一综述。

This article reviews the current situation on several DNA vaccines .

猪囊尾蚴DNA疫苗在猪组织中的分布

DNA Taenia solium cysticercosis vaccine : tissue distribution in porcine

猪囊尾蚴抗原DNA疫苗诱导的免疫保护效应

Protective immunity induced by DNA vaccine of Cysticercus cellulosae antigen

猪囊尾蚴抗原与猪白细胞介素-4基因融合DNA疫苗载体的构建

Construction of DNA vaccine including a chimeric gene encoding cysticercus cellulosae antigen and porcine interleukin 4

猪囊尾蚴特异性抗原编码cDNA的阶段特异性表达

Stage specific expression of antigen encoded cDNA of Cysticercus cellulosae

猪囊尾蚴特异性抗原基因的cDNA的分子克隆

Molecular Cloning of a cDNA Encoding a Specific Antigenic Polypeptide of Cysticercus Cellulosae

猪囊尾蚴cDNA表达文库的免疫筛选

Immune screening of cDNA library from Cysticercus cellulosae

方法：猪囊尾蚴cDNA表达文库中筛选出的β－半乳糖苷糖－猪囊虫cDNA编码的融合蛋白为抗原，进行简化的westernblot分析。

METHODS : Fusion protein of β galactosidase Cysticercus cellulosae cDNA was analysed with simplified Western blot .

用质粒表达载体pSPORT1构建了猪囊尾蚴cDNA文库。

The cDNA library from Cysticercus cellulosae had been constructed with the vector ( pSPORT-1 ) .

猪囊尾蚴囊液抗原制备及ELISA检测条件的优化猪带绦虫囊尾蚴囊液谷胱甘肽S转换酶活性测定

Prep of Cysticercus cellulosae Cyst fluid antigen and optimization of ELISA Detection of glutathione S-transferase in Cysticercus cellulosae

结论猪囊尾蚴DNAPCR检测方法的建立为猪囊尾蚴感染的临床诊断和病原流行病学调查提供了实验基础。

Conclusion The establishment of PCR assay for cysticercus cellulosae DNA detection provides laboratory basis for clinical diagnosis and etiological investigation of this pathogen .

结论筛选鉴定的基因推测为猪囊尾蚴RNA聚合酶亚基基因，而且在不同物种间很保守。

Conclusion The protein , RNA polymerase subunit encoded putatively by the clone , is high conservative in different species .

免疫金银法标记抗猪囊尾蚴囊液抗原McAb与猪囊尾蚴特异性结合的定位研究

Study on localization of cystic fluid antigen of Cysticercus cellulosae using mcab-igss

试验三目的：探讨仔猪人工感染猪囊尾蚴后外周血中CD3~+、CD4~+、CD8~+T淋巴细胞的变化规律。

Objective : Objective : to explore the dynamic changes of level of antibody serum in peripheral blood of piglets with Cysticercosis cellulosae by artificial infection .

猪囊尾蚴线粒体NADH脱氢酶的基因克隆

Cloning and Characterization of Mitochondrial NADH Dehydrogenase Gene of Cysticercus cellulosae

融合蛋白dot-ELISA法检测猪囊尾蚴病人循环抗体

A dot-ELISA test using fusion protein antigen for detecting cysticercosis antibody

结论猪囊尾蚴全囊尿素溶性纯化抗原是较理想的囊虫病ELISA诊断抗原，有诊断应用价值。

Conclusion The data suggested that purified urea-soluble antigen from C. cellulosae the diagnostic antigen , was an ideal one for this disease diagnosis with ELISA .

猪囊尾蚴抗原基因6H的克隆、表达及鉴定

Molecular cloning , expression and identification of 6H gene from the cDNA library of cysticercosis cellulosae

重叠延伸PCR法扩增猪囊尾蚴AgB基因及其在BHK-21细胞中的表达

Cloning of Cysticercus cellulosae AgB Gene by Using Splicing Overlap Extension PCR Method and Expression in BHK Cells

目的探讨猪囊尾蚴副肌球蛋白（又称为AntigenB，AgB）cDNA中CpG序列的免疫激活作用。

Objective To analyse the immunostimulatory activity of CpG sequences in cysticercus cellulosae paramyosin ( also named Antigen B , AgB ) cDNA .

γ-干扰素（IFN-γ）/白细胞介素-4（IL-4）在人体猪囊尾蚴感染中免疫调控的作用及意义。

To investigate the role and significance of Thl / Th2 cytokines ( IFN - - γ / IL-4 ) in immune regulation of cysticercus cellulosae infection .

国内外研究证明，用猪囊尾蚴AgB重组疫苗或DNA疫苗免疫小鼠或猪能有效地抵御猪带绦虫虫卵的攻击感染。

Inoculation of cysticercus cellulosae AgB recombinant or DNA vaccine can efficiently prevent mouse or pig from infecting of Taenia solium has been proved as many researches in both China and abroad .

方法用分子筛分离提纯猪囊尾蚴囊液粗抗原，用ELISA法检测未经治疗和经1～3,4～6和7～9疗程治疗的4组确诊脑囊虫病患者血清中的总IgG和IgG4。

Methods ELISA was used for the detection of the total IgG and IgG 4 in serum of patients without treatment , or with 1 ~ 3 treated , 4 ~ 6 treated , and 7 ~ 9 treatment .

以EITB技术逐一分析了猪囊尾蚴两种抗原制备物中各多肽的一些生化特性和抗原性。

Some biochemical characteristics and antigenicity of the polypeptides of two antigenic prepared products of Cysticerci cellulosae were analysed step by step with EITB ech-nique .

本文报告应用抗猪囊尾蚴单克隆抗体（McAb）4F8-ELISA对231例脑囊虫病患者脑脊液中循环抗原（CAg）检测的结果。

The results of detection of circulating antigen ( CAg ) in cerebrospinal fluids ( CSF ) of 231 cerebral cysticercosis patients with McAb ( 4F8 ) - based ELISA were reported and compared with the case histories , clinical manifestations and CT scans .

应用猪囊尾蚴头节冰冻切片抗原进行免疫酶染色试验（IEST）。

Frozen section of Cysticercus cellulosae scolex was used as antigen in Immunoenzymatic Staining Test ( TEST ) for the detection of antibody in sera from patients with cysticercosis .

猪囊尾蚴细胞疫苗区域试验报告

Regional Experiment Report about Pig 's Cyst Cercaria in Cell Vaccine

吡喹酮对体外猪囊尾蚴作用的电镜观察

Electron microscopic observation on cysticercus cellulosae treated with pyquiton in vitro