原头蚴

发现介于原头蚴与囊泡之间的形态。

Cysts were found with morphology between protoscolex and metacestode .

细粒棘球绦虫（E.g）原头蚴的简易培养

A Simple Method of Culture of Protoscolices of Echinococcus Granulosus in Vitro

方法：应用玻璃匀浆器轻研磨法和0.25%胰蛋白酶4℃消化约4～6h后，获得原头蚴体细胞。

Methods : combing the glass pestle with 0.25 % trypsin at 4 ℃ to digest protoscolices about 4 ~ 6 hours later , the body cells can be obtained .

方法口服复方消包片或阿苯达唑[25mg/（kg·d）]3个月，然后，采用粗管经皮穿刺吸刮包虫囊肿内容物坏死原头蚴破碎溶解内囊及子囊。

Methods Oral Fu Fang Xiao Bao tablet or Albendazole , 25mg / kg per day , for 3 months was followed by percutaneous puncture insertion of wide bore tube for suction and curettage of liquefied necrotic and fragmented scoleces of hydatid cyst and daughter cysts .

方法孔雀绿染色法：将原头蚴分别用1%、2%和4%孔雀绿水溶液，在室温或36℃染色24h、48h和72h。

Methods Malachite green stain-method : the protoscolices were stained at room temperature or 36 ℃ for 24 h , 48 h and 72 h with 1 % , 2 % and 4 % malachite green solution respectively .

用原头蚴作抗原作间接荧光抗体试验（IFA）诊断包虫病，并与临床常规检查皮内试验（ID）、间接血凝试验（IHA）、对流免疫电泳试验（CIEP）的结果比较。

Protoscolices were used as antigen with IFA diagnosis of hydatid disease , which was compared with routine diagnosis of intradermal test ( ID ), indirect haemagglutination ( IHA ) and counter-immunoelectrophoresis ( CIEP ) tests .

细粒棘球蚴-原头蚴体外培养成囊模型的建立

Establishment of the in vitro cultivation model of protoscolex of Echinococcus cyst

目的探索制作结构清晰、颜色鲜明、能长期保存的细粒棘球绦虫原头蚴标本的方法。

Objective To explore the method of making the clear structure , bright-color and long preservation specimen of Echinococcus granulosus protoscolices .

方法：分别采用0.25%胰蛋白酶消化法、玻璃匀浆器研磨法、玻璃匀浆器轻研磨与0.25%胰蛋白酶消化结合法对原头蚴体细胞进行分离。

Methods : The protoscolices were treated by 0.25 % trypsin , the glass pestle , and mixed way which combined the glass pestle with 0.25 % trypsin , respectively , in vitro .

分离出的原头蚴体细胞在透射电镜下可见到实质细胞、单核的未分化细胞、糖原细胞和肌细胞，4种细胞具有其各自的超微结构特点。

Under the transmission electron microscope , we could see the 4 kinds of the body cells , they are parenchymatous cells , single nucleus undifferentiated cells , glycogen cells and muscle cells . These cells had their own characteristics .

在实验室条件下，给幼犬吞食一定量的原头蚴和含生发层的包囊，经51～109天可在犬粪便中查出孕节或在小肠里检出成虫，感染成功率为77.3%。

Under laboratory conditions , after the dog ate a certain amount of Protoscolexes and capsules with germinal layers , gravid segment could be found in dog 's excrements and the mature worms identified in 51 ~ 109 days , the rate of infection 77.3 % .