激光捕获显微切割

激光捕获显微切割（LaserCaptureMicrodissection，LCM）与基因芯片是后基因组时代新出现的两种技术。

Laser capture microdissection ( LCM ) and microarray are two new immerging techniques in post-genome era .

激光捕获显微切割（LaserCaptureMicrodissection，LCM），是一项利用激光在显微镜下从组织切片中分离单一类型细胞群的技术。

Laser capture microdissection ( LCM ) is designed to isolate homogeneous populations of cells from histologic sections of complex tissues under direct microscopic visualization .

目的探讨激光捕获显微切割分离大鼠脑微血管和神经元联合功能分类表达谱cDNA芯片技术在脑缺血研究的应用。

Objective To develop a method to analyze functional grouping cDNA microarray gene expression in microvessels and neurons from rat brain using laser capture microdissection ( LCM ) .

目的探索激光捕获显微切割技术（LaserCaptureMicrodissection，LCM）定位分离心房细胞，并对提取的微量RNA进行β-actin及GAPDH基因扩增。

Objective To use the laser capture microdissection ( LCM ) to isolate the pure atrial cells from heart . To extract tiny amount RNA and amplify the genes of β - actin and GAPDH .

新的蛋白质组学技术如激光捕获显微切割（LCM），串联质谱、二维液相分离-质谱鉴定等加速了乳腺癌研究的进展。

The research of breast cancer will be developed more quickly by means of newer proteomic technologies such as laser capture microdissect ( LCM ), tandem MS and 2D-LC-ESI-MS , etc.

激光捕获显微切割（LCM）是近年来发展起来的从杂乱的多细胞组织中分离特定细胞群的新技术，分离的细胞群还可应用于后续的DNA、RNA和蛋白质的分析。

Laser Capture Microdissection ( LCM ) was a novel technique recently developed to isolate pure population of specific cells from miscellaneous tissues . The captured cell population can be used in the subsequent assay for DNA , RNA and protein .

近年发展起来的激光捕获显微切割（LaserCaptureMicrodissection，LCM）技术可以在显微镜直视下快速、准确地获取所需的单一细胞亚群，甚至单个细胞，从而成功解决了组织中细胞异质性问题。

Laser capture microdissection ( LCM ) is a novel technique that can be applied to obtain pure targeted cell subgroup or even a single cell quickly and precisely under the microscope , thus the problem of tissue heterogeneity in molecular analysis can be tackled successfully .

运用激光捕获显微切割对肝细胞癌与肝内胆管癌蛋白质组的分析

Proteomic analysis of hepatocellular carcinoma and cholangiocarcinoma by laser capture microdissection

激光捕获显微切割技术应用研究新进展

New Developments in Application of Laser Capture Microdissection

应用激光捕获显微切割及蛋白质组学技术筛选鼻咽癌间质相关蛋白

Screening for the Stroma-related Proteins in Nasopharyngeal Carcinoma by Laser Capture Microdissection and Proteomic Analysis

基因芯片联合激光捕获显微切割技术在脊髓背根神经节基因表达研究中的应用

Combining Laser Capture Microdissection and Microarray to Analyze the Gene Expression of Rat Dorsal Root Ganglion Sensory Neurons

激光捕获显微切割技术纯化的肺鳞癌原发灶与淋巴结转移灶肿瘤细胞的比较蛋白质组学

Comparative proteome analysis of laser capture microdissection for purified primary tumor and lymph node metastatic tumor in human lung squamous carcinoma

方法：通过激光捕获显微切割技术获取食管鳞癌和正常食管上皮细胞，采用双向凝胶电泳方法分离蛋白质，采用计算机辅助的图像分析技术比较分析凝胶图像。

Methods : Pure human esophageal squamous cell carcinoma cells and normal esophageal epithelial cells were obtained by LCM , protein were separate by Two dimensional polyacrylamide gel electrophoresis ( 2-DE ) technique , and computer-assisted image analysis were used to analyze the images of polyacrylamide gels .