核酸杂交

核酸杂交法检测三种化合物对Ⅱ型单纯疱疹病毒DNA合成的影响

Nucleic acid hybridization technique for measurement of the effect of three compounds on herpes simplex virus type ⅱ DNA synthesis

尿嘧啶DNA糖苷酶防止PCR产物污染及其对核酸杂交的影响

Prevention of contamination in PCR products by using uracil-DNA-glycosylase before amplification and its influence on nucleic acid hybridization

微板核酸杂交-酶联显色检测性病沙眼衣原体DNA

Detection of Chlamydia Trachomatis DNA Using PCR-Microplate Hybridization-ELISA Enzyme mimics

核酸杂交检测HBVdna及基因分型

Detection and genotyping of HBV DNA by polymerase chain reaction and hybridization

采用PCR微板核酸杂交-ELISA技术进行HBVdna基因分型的研究

Study on genotypes of HBV DNA using PCR microplate hybridization-ELISA

微板核酸杂交-ELISA方法对肝炎病人血清中HBVdna的定量检测与分析

Quantification and analysis of HBV DNA in hepatitis patient serum using sandwich hybridisation-ELISA assay on microplate

用核酸杂交检测心肌炎小鼠心肌中柯萨奇B病毒RNA

Detection of Coxsackievirus B Genome in Myocardium of BALB / c Mice with Experimental Myocarditis by cDNA-RNA Hybridization

微板核酸杂交技术对江西地区HCV基因分型的观察

The Study of Genotypes of HCV RNA Using Micro-plate Hybridization-ELISA

采用ELA、PCR、核酸杂交方法。

ELA , PCR and nucleic acid hybridization methods were used .

应用核酸杂交（dotblot）分析50例胃癌及其对应正常组织c－myc癌基因扩增情况。

Amplification of c-myc in stomach carcinoma and its adjacent normal tissue was analysed by dot blot .

百合无症病毒的RT-PCR和核酸杂交检测及其外壳蛋白在大肠杆菌中的表达

RT-PCR and Dot-Blot Hybridization Detection of Lily Symptomless Carlavirus and the CP Gene Expression in Escherichia Coli

线探针核酸杂交分析贵州地区HCV感染株基因型

Analysis of hepatitis C virus genotypes in Guizhou area , using second generation line probe assay

异羟基洋地黄毒甙元（Digoxigenin）标记的RNA探针在丁型肝炎病毒核酸杂交中的应用

Use of Digoxigenin Labelled RNA Probe in Hepatitis D Virus RNA Hybridization

应用RNA原位核酸杂交检测乳腺癌石蜡切片中ERmRNA、PRmRNA表达

Detection of ER mRNA and PR mRNA expression in paraffin-embedded sections of breast carcinoma by RNA nucleic acid hybridization in situ

用核酸杂交法检测了酞丁安等三种化合物，对Ⅱ型单纯疱疹病毒DNA合成的影响。

A nucleic acid hybridization technique for measurment of the inhibitory effect of Tai-Ding-An , Aclacinomycin A and B on herpes simplex virus type I DNA synthesis is described .

目前的分子生态学技术主要有核酸杂交分析技术、特异性PCR扩增技术、DNA序列分析、基因芯片技术等。

At present , molecular ecology technology mainly is nucleic acid hybridizing detection , PCR amplification , DNA sequence analysis , gene chip , and other technology .

本文叙述一种将辣根过氧化物酶（HRP）与单链DNA交联以制备非同位素核酸杂交探针的新方法。

A new method for constructing nonradioactive hybridization probes was studied , which allowed crosslinks to be producedbetween HRP and single-stranded DNA .

目的：评价原位核酸杂交技术在人乳头瘤病毒（HPV）检测中的敏感性。

Objective To evaluate the sensitivity of in-situ hybridization ( ISH ) for detecting human papillomavirus ( HPV ) .

DTPA－Eu~（3＋）螯合物在核酸杂交分析中的应用

The Application of DTPA-Eu ￣( 3 + ) Complex to DNA Hybridization Assay

采用免疫组化和原位核酸杂交法检测瘤细胞内EBV基因表达产物。

Immunohistochemical and in situ nucleic acid hybridization methods for detection of EBV gene encoded products were performed .

核酸杂交和McAb－IFA快速检出鉴定白纹伊蚊体内登革热病毒的研究

Use of McAB ─ IFA and Nucleic Acid Hybridization for Detecting Dengue Virus in Infected Aedes Aegypti Mosquitoes

本文报道地里纤恙螨雄虫人工接种恙虫病立克次体后与雌虫配对培养和传代，应用幼虫叮咬小鼠分离立克次体和PCR结合核酸杂交（核酸杂交检测PCR产物）检测子代体内立克次体的结果。

This paper reports the results of the detection of Rickettsia tsutsugamushi in the offsprings of Leptotrombidium deliense which male parents were infected through inoculating by using PCR combine DNA-DNA hybridization technique and traditional technique .

方法应用RNA原位核酸杂交（RISH）和免疫组化（IHC）检测技术。

Methods 278 cases were examined by RNA nucleic acid hybridization in situ ( RISH ) and immunohistochemistry assay ( IHC ) .

方法采用PCR、核酸杂交和酶联显色技术以及荧光定量PCR技术，分别对123例慢性乙型肝炎（CHB）患者进行HBVdna基因分型和血清HBVdna水平的测定。

Methods The genotype of HBV and serum level of HBV DNA were detected by PCR , ELISA and quantitive fluorescent PCR in 123 patients of chronic hepatitis B ( CHB ) in Quanzhou area .

报道了靶DNA／生物素化λDNA探针／亲合素／Bio－BSA（TG）－DTPA－Eu~（3＋）体系在核酸杂交分析中的应用。

Here we reports a new detection method for λ DNA through the system of target DNA / Bio-DNA probe / Avidin / Bio-BSA ( TG ) - DTPA - Eu3 + .

RT-PCR和核酸杂交检测百合病叶总RNA的稀释限度分别为1：10~（-6）和1：10~（-2）。检测方法快速，灵敏。

The sensitive level of RT-PCR and dot-blot hybridization detection of the total RNA of infected lily leaves by LSV was 1:10 ~ ( - 6 ) and 1:10 ~ ( - 2 ) respectively .

方法：首先比较PCR-微板核酸杂交-ELISA法和S基因序列测定分析法检测43例HBV感染者基因型的结果；

Methods : The HBV genotype of 43 patients with HBV infection was determined by using of PCR microplate hybridization-ELISA technique and direct sequencing of HBV DNA S gene , and the two HBV genotyping method were compared .

核酸杂交主要用于PCR扩增结果的确诊以及提高诊断的敏感性，但由于实验仪器设备要求较高，尚未直接用于临床标本的检测。

The nucleic acid hybrid mainly uses in PCR to expand increases the result to diagnose as well as the enhancement diagnosis sensitivity , but because tests the meter . The equipment request is higher , not yet directly uses in the clinical specimen examination .

利用核酸杂交技术证明了在CHL细胞中存在CENP－B基因，并研究了咖啡因诱导MUG细胞形成过程中CENP－B的基因表达。

We demonstrated for the first time the existence of CENP-B gene in CHL cells by Southern blot and examined the CENP-B gene expression during the process of MUG cell induction by caffeine .

核酸杂交结果表明，宫颈鳞癌中，HPV-16的感染率较高，HPV-18的感染率较低，二者比较差异有显著性（P＜0.01）；

The infection rate of HPV-16 was higher than that of HPH-18 in cervical squamous cell carcinoma , there was significant difference between the 2 virus types ( P < 0 . 01 );