核小体
- 名nucleosome
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核小体只提供了DNA压缩的第一级水平。
The nucleosome provides only a first level of condensation of the DNA .
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在真核生物中,DNA按左手手征性的方式,缠绕在组蛋白八聚体的周围,形成稳定的核小体结构。
In all eukaryotes , DNA wraps around a histone octamer in a left-handed way , forming a stable nucleosome structure .
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基因组DNA包含了绝大部分的遗传物质,对于真核生物而言,DNA缠绕组蛋白八聚体形成核小体,进而被包装在染色质内。
In eukaryote , genomic DNA is packaged in chromatin by twining with histone octamer .
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在真核生物中,组蛋白是染色质基本结构一核小体中的重要组成部分,其N末端氨基酸残基可发生乙酰化等共价修饰。
Covalent modifications , such as acetylation occur in the N-terminal amino acids of histones .
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钠离子浓度对核小体纤维结构影响的MonteCarlo模拟
Monte Carlo simulation of chromatin fiber conformation affected by ionic strength
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染色质修饰包括核小体组蛋白N末端的共价修饰和DNA的胞嘧啶甲基化。
Chromatin modification consists of covalent modifications of the N-terminal tails of the nucleosomal histones and DNA cytosine methylation .
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还有实验数据揭示在一个核小体中,组蛋白和DNA相互作用有三个作用很强烈的区域。
Furthermore , experimental data revealed that three regions of strong interactions between core histones and DNA sequences in a nucleosome .
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表达核小体Th表位减毒鼠伤寒沙门菌株的构建
Construction and expression of the attenuated Salmonella typhimurium strain expressing the nucleosome Th epitope
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DNA作为生命的遗传蓝图,必须被解码或被“阅读”,甚至在被紧密“打包”放进核小体的时候也是如此。
As the genetic blueprint of life , DNA must be deciphered or " read ," even when densely packed into nucleosomes .
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这些帮助DNA堆积的蛋白叫做组蛋白,DNA围绕组蛋白形成的基本堆积单位叫做核小体。
These proteins helping DNA package are called histones and the basic element that formed by DNA and histones is called nucleosome .
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烟草拟核小体组装蛋白cDNA的克隆、表达及其蛋白产物的纯化
Cloning and Expression of a Putative Tobacco Nucleosome Assembly Protein cDNA and Purification of Its Product
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在精子生成过程中,核小体组蛋白被其他更为碱性的蛋白所替代,使DNA的结构变得更为集缩。
During spermatogenesis the histones in the chromatin are replaced by other more basic proteins leading to a more condensed structure of the DNA .
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核心过程的表观遗传的产业包括DNA甲基化、组蛋白改性、核小体重建、核染色质动力学和互动子都存在。
Core processes of epigenetic inheritance include DNA methylation , histone modification , nucleosome remodeling , nuclear dynamics and chromatin interaction with non-coding RNAs .
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DNA序列上核小体的准确定位在基因转录,mRNA剪接,DNA复制和DNA修复中扮演着重要的角色。
The precise positioning of nucleosomes along the DNA plays important roles in gene transcription , mRNA splicing , DNA replication and DNA repair .
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依赖于ATP的染色质改构复合物对核小体结构的调整是基因转录的必要前提。
It is necessary for facilitating transcription to disrupt nucleosome structure by the ATP-dependent nucleosome remodeling complexes .
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在真核细胞中,核小体的核心结构组蛋白被DNA紧密缠绕,但是这种紧密的结合有时也可能变得松散,以利于其他蛋白与DNA的结合。
Histone and DNA bind together to form the nucleosome , but this tight binding can loose at some times to facilitate other proteins to interact with DNA .
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所有这些信息暗示,DNA序列中存在一个与组蛋白相互作用的模体集合,我们称之为核小体定位模体。
These evidences implied that there was a motif set which were interacted with histone octamers exist on the DNA sequences ; we termed them " nucleosome binding motifs " .
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通过分析发现这些模体具有较高的GC含量和柔性,在实验给出的核小体定位序列中,它们出现的频率非常显著。
The analysis revealed that these motifs have higher GC content and flexibility , and their frequencies are significantly high in experimental nucleosome positioning sequences .
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这个结果表明,看家基因功能位点附近的核小体定位和重塑信号比Ensemble基因强烈。
This result indicates that nucleosome positioning and remodeling signal near the functional site of housekeeping gene is stronger than Ensemble genes .
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核小体动力学主要研究DNA与组蛋白的相互作用下核小体受力、结构形成过程,是蛋白质组研究计划的核心研究内容之一。
Nucleosome dynamics , one of the core areas of histone protein research projects , is largely concerned with the interaction between DNA and histone octamer and properties of the formation of nucleosome complex .
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抗核小体抗体及抗C1q抗体在LN发病中起重要的作用。
Anti-nucleosome antibodies and anti-C1q antibodies are suggested to play a role in the pathogenesis of LN .
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而未被清除的凋亡细胞会积聚诱发二次坏死,细胞质膜破裂释放出大量内容物,如核小体碎片、DNA、蛋白质、组蛋白成分,引发炎症反应及自身免疫性疾病。
Accumulation of uncleared apoptotic cells results in " secondary necrosis " and the release of cytoplasmic content , such as pieces of nucleosome , DNA , protein , histone composition , causing inflammation and autoimmune diseases .
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染色质重构使染色质组织结构发生一系列重要的变化,如染色质去凝集,核小体变成开放式的疏松结构,使转录因子等更易接近并结合核小体DNA,从而调控基因转录等。
Remodeling resultes in a series of important changes in chromatin structure , e.g. loosing the condensed chromatin and opening the structure of nucleosome , which leads to increased accessibility of the transcription factors to nucleosomal DNA .
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结论在LN患者中,存在着抗核小体抗体、抗C1q抗体的高表达。
Conclusion In patients with LN , anti-nucleosome antibodies and anti-C1q antibodies are significantly higher than control groups .
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分析了人类看家基因功能位点附近的核小体结合模体的分布,并与Ensemble基因比较了它们之间的相同点和差异。
Analyzed the distribution of nucleosome binding motifs near the functional sites of human housekeeping genes and compared the similarities and differences with the Ensemble genes .
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在真核细胞中,所有的基因组DNA与高度进化保守的碱性蛋白&组蛋白以及多种组蛋白相关染色质因子组装构成核小体结构,重复此单元而形成染色质结构。
In eukaryotic cells , all genomic DNA with a high degree of evolutionary conservation of basic protein & histone and histone-related chromatin assembly factors constitute a nucleosome structure ; the repetition of this element forms chromatin structure .
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随后的一系列功能试验显示,SET/TAF-Iβ是一个具有多种功能的蛋白质,参与到众多的生理途径中。包括组蛋白结合,核小体组装,DNA复制、转录激活以及凋亡等等。
A series of functional tests show that SET / TAF-I β is a multi-functional protein , involved in many physiological pathways , such as the histone binding , nucleosome assembly , DNA replication , transcription and apoptosis .
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应用有机汞亲和层析柱分离经微球菌核酸酶有限制消化的HL-60细胞核,得到有机汞层析柱亲和及不亲和的2个核小体组分。
The unbound and bound fractions of nucleosome from HL-60 cell nuclei digested with micrococcus nuclease were separated by Hg-affinity chromatographic column .
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儿童系统性红斑狼疮抗核小体抗体和抗dsDNA抗体的检测和意义
Detection and Significance of Anti-nucleosome Antibodies and Anti-dsDNA Antibodies of Systematic Lupus Erythematosus in Children
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尽管有许多因素可以影响核小体的定位,但现有的核小体定位数据显示,核小体对特殊的DNA序列有高度的亲和力,DNA本身的序列在体内核小体定位中扮演很重要的角色。
Although nucleosome positioning can be influenced by several factors , present nucleosome positioning data showed that nucleosomes have higher affinity for particular DNA sequences and intrinsic DNA sequence preference plays an important role in nucleosome positioning in vivo .