振荡培养

对分离出的高效菌种的特性进行了较为详细的研究，主要研究结果是：110转／min振荡培养有利于菌种的生长繁殖；

Detailed study has been carried out on specific property of the separated efficient bacteria , the chief results are that shaking culture at 110r / min is favorable for the growth and reproduction of the bacteria ;

适宜的发酵方式为先130r/min振荡培养10h，后静置培养10d。

The optimum fermentation condition was : first shaking culture for 10 hours , and then static cultivation for 10 days .

采用液体振荡培养，研究了Mn、B、Zn、Cu和Mo5种微量元素复合物对4种肥料微生物生长的影响。

Using the liquid vibration culture , the effect of trace element mixture on growth of several kinds of fertilizer microbes was studied .

在增添细胞分裂素和生长素的MS或改良B5液体培养基里振荡培养，明显地促进了胚状体根的生长和茎的发育。

The root growth and the shoot development of embryoids were promoted markedly in the MS or B5 liquid medium supplemented with cytokinin and auxin by shake culture .

3L摇瓶振荡培养，B亚单位产量可达16μg/ml。

The yield of the subunit B was 16 μ g / ml in 3 litre shaking flask .

基于Modbus协议的恒温振荡培养箱控制器设计与实现

The Design and Realization of Constant Temperature Incubator Shaker Controller Based on Modbus Protocol

210rpm振荡培养72h，，在此发酵条件下，该菌产生的纤溶活性可达到820U／mL。

Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth .

对不同培养基质、培养时间、培养初始pH值、振荡培养、光照等进行研究，探索泽兰尾孢菌的产毒条件。

The phytotoxin producing conditions of Cercospora eupatorii were experimented with different media , culture durations , initial pH values of the media , shaking intensity and illumination conditions .

进而对这51株硅酸盐细菌的溶磷解钾能力进行了测定，探讨了细菌液体振荡培养前后pH值和菌数变化与溶磷、解钾量的关系。

The phosphorus and potassium releasing ability tested , and the relation between pH , the bacterial amount and the phosphorus releasing capacity , the potassium releasing capacity was performed .

菌株A1和菌株F1都能以羽毛为唯一的碳源、氮源和能源生长，将其接种于经过灭菌处理的羽毛培养基中，振荡培养4～5天后可使整枝羽毛完全消失。

Both strain A1 and strain F1 could grow on feathers as the sole carbon , nitrogen and energy sources .

采用振荡培养实验和活性污泥模拟实验对大豆油乙氧基化合物（SOE）进行了生物降解研究。

The biodegradability of soybean oil ethoxylates ( SOE ) was studied by means of shake culture test and activated sludge simulation test .

实验并分析了静置培养和振荡培养2种培养方式处理下，桑种子萌发状态及萌发过程中内源激素含量和超氧化物歧化酶（SOD）活性的变化。

After that , we observed seed germination and measured variations of endogenous phytohormone content and SOD ( superoxide dismutase ) activity during germination of the seeds under static and shaking cultural conditions .

考察不同培养条件（包括培养基的种类；培养基中铬的添加浓度；培养基起始pH值；振荡培养时间；酵母添加量；培养温度等）对制备富铬酵母的影响。

Each kind of influence factors on preparation of chromium-enriched yeast ( kind of culture medium , concentration of chromium ion , initial pH of culture medium , cultivate time , yeast adding quantity , cultivate temperature ) are studied .

以最佳培养基为发酵培养基，于37℃、160r/min振荡培养48h，酶活达2519.35U/mL；

Protease activity reached 2 519.35 U / mL under the conditions of 37 ℃, 160 r / min , 48 h in the optimal fermentation medium .

适宜的醋酸发酵条件为温度33~36℃，酒精浓度6.0%，接种量6.0%，起始pH值6.0，在转速140r/min条件下振荡培养72h左右桑果保健醋酸度适宜，口感、风味良好。

The optimum temperature for acetic acid fermentation is 33 ~ 36 ℃, alcohol density 6.0 % , inoculation amount 6 % , starting pH degree 6.0.The rotational speed is 140 r / min and the oscillation period is 72 h.

HMX-3对活性艳红X-3B脱色的最适温度为25℃~35℃，最佳pH值为6~8，且振荡培养较静置培养条件下的脱色率高；

The most preferable temperature of HMX-3 to adsorb Active Brilliant Red X-3B was 25 ~ 35 ℃, and pH value was 6 ~ 8.And the decolorization rate at the condition of agitation was higher than stationary condition .

限氮振荡培养时，起始pH值4.5，温度37℃转速175r/min培养48h后降低温度为30℃，转速为100r/min，100mL三角瓶装液量30mL，所得酶活可达980U/L。

In N-limited shaking culture , initial pH value was 4.5 , temperature 37 ℃, the rotational speed of 175 r / min for 48 h , then temperature dropped to 30 ℃ and ( 100 r / min. )

毛状根在1/2MS改良培养液中振荡培养，第8d进入快速生长期，20d可增长8倍。

Hairy roots were cultured on amend 1 / 2 MS for 8 days will be in fast growth period , and which cultured for 20 days will increased for 8 times .

方法：分别用添加02M葡萄糖的人唾液振荡培养白色念珠菌、热带念珠菌及季也蒙念珠菌，定时取样测定生长量、pH值、胞外蛋白酶活性及唾液总蛋白浓度。

Methods : Growth mass , pH value , SAP activity and total salivary protein concentrations were measured periodically when three Candida spp . were incubated on a shaker in human saliva supplemented with 0.2M glucose , respectively .

F.solani液体培养的最佳产毒条件为：25℃振荡培养15d，培养基对F.solani产毒的影响不大。

: The optimum conditions of F. solani producing toxin are : 25 ℃ for 15 day by vibrating and there are no difference of the three liquid mediums .

在以纤维素为碳源的培养液中，28℃振荡培养4d，滤纸纤维素失重率分别达57.53%和54.92%，未经处理的稻草秆纤维素失重率为38.82%和36.21%。

They grew in a liquid medium with cellulose as sole carbon source . Shaking at 28 ℃ for 4 days , the cellulose of filter paper were decomposed 57.53 % and 54.92 % respectively ; cellulose from untreated rice straw stalk 38.82 % and 36.21 % .

结果表明，适宜马铃薯晚疫病菌粗毒素培养的培养基为黑麦液体培养基，培养基pH值为6.0-7.0，培养温度20-22C，在黑暗条件下连续振荡培养16d时产生的粗毒素的毒性最强。

The results showed that the favorable culture conditions were rye liquid medium , pH value 6 . 0-7 . 0 , culture temperature 20-22 C with successive darkening and shaking during culture . The production of crude toxin reached the highest level on the 16th day of culture .

最适产酶条件为：温度30℃，起始pH7.0，接种量2%，150mL三角瓶装50mL，150r/min振荡培养72h。

The optimum cultural condition for enzyme production in shaking flask : temperature 30 ℃, initial pH 7.0 , medium volume 50ml / 150ml flask , inoculum concentration 2 % , shaking speed 150r / min , culture period 72 hours .

其发酵较适宜的条件为：起始pH值为6.0，接种量10%，500mL摇瓶装液量150mL，温度25℃，150～180r·min-1振荡培养144h即可结束发酵。

The suitable culture conditions were as follows : initial pH 6.0 . inoculation volume 10 % . the volume of shake flask 150mL / 500mL . temperature 25 ℃, rotation rate 150 ~ 180 r · min-1 and culture period 144h .

选择8种培养基进行振荡培养，从中选出YMA、YMA磷酸氢二铵、土壤浸出液、黄豆饼浸出液4种培养基进行深层发酵培养。

Eight kinds of culture media were designated to carrying on shaking culture , selecting the YMA , the YMA ammonium phosphate dibasic , the extraction of soil , the defatted soybean leaching solution four kinds of culture medium carried on submerged fermentation culture .

胚性愈伤组织在悬浮振荡培养中可保持分生能力。

Suspension culture was established from the embryogenetic callus of walnut .

固体平板和液体静置的培养方法优于液体振荡培养；

Solid flat and liquid stationary methods were better than liquid vibration ;

液体振荡培养繁殖草莓无毒苗

Propagation of virus-free strawberry plants in liquid shake culture

次级小鳞茎采用液体振荡培养可快速增重。

By liquid - quivering culturing , the weight increase can be accelerated .

振荡培养还可使类原球茎发育同步化。

The development of PLBs could also be synchronized by using suspension culture .