异硫氰酸苯酯

以异硫氰酸苯酯为衍生剂，水浴加热反应，用正己烷处理水浴反应产物，这些柱前衍生的方法，对氨基葡萄糖衍生化后进行检测。

With phenyl isothiocyanate as derivative agent , water-bath heating reaction , reaction products treated by n-hexane ment , these pre-column derivatization method for testing glucosamine .

方法：在碱性条件下，氨基酸与异硫氰酸苯酯反应，生成异硫氰酸苯？

Methods : In alkaline condition , reaction between amino acids and phenylisothiocyanate formed a derivant of phenylisothiocyanate-amino acid .

采用异硫氰酸苯酯柱前衍生高效液相色谱法测定桑叶药材中16种氨基酸的含量，采用ICP-AES技术测定了24批次桑叶药材中金属元素的含量。

Determinated the content of 16 kinds of amino acids in different areas of mulberry leaves by HPLC with the method of precolumn derivation .

磺基异硫氰酸苯酯化学辅助方法对新蛋白质进行从头测序

De Novo Sequence of Novel Protein Based on Chemically-assisted Method Using 4-Sulfophenyl Isothiocyanate Derivatization by Mass Spectrometry

但用GC/MS分析该晶体为异硫氰酸苯酯，这可能是在GC/MS分析过程中二苯硫脲发生了中性丢失苯胺的缘故。

But the GC / MS analysis showed that it is phenyl isocyanate . This may be due to neutral loss of aniline in the cleavage of diphenyl thiourea in GC / MS analysis .

实验结果表明二苯硫脲质谱裂解中可能发生了中性丢失，丢失了中性分子苯胺，生成的异硫氰酸苯酯分子离子由于π电子共轭而致稳。

The results showed that neutral molecule of aniline may be lost in the cleavage of mass spectra , while the remainder molecular ion of phenyl iso-thiocyanate became steady due to the conjugate of π electron .

利用Pd/C催化加氢的方法，将中间体硝基化合物3还原成相应的中间体氨基化合物4，制得的中间体氨基化合物，无需进一步纯化即可直接与异硫氰酸苯酯反应，得到目标产物5。

The intermediates 3 , nitro compounds , were reduced into corresponding intermediate amido compounds 4 by Pd / C catalysis-hydrogenation . The target compounds 5 ware obtained by the reaction phenylisothiocyanate with the intermediate amido compounds 4 , which do not have been separated .