高通量筛选

抗抑郁药5-HT重摄取抑制剂高通量筛选方法的建立与应用

Establishment and application of high throughput screening for discovery of new antidepressants

p53直接调控microRNA及其靶基因的高通量筛选

The High Throughput Screening of Direct Regulatory microRNA and Their Target Genes

高通量筛选DNA加合物位点方法的探讨

Study on a high throughput method of screening adducted site of DNA adducts

该方法有望成为Dam甲基化酶的抑制、激活因子的高通量筛选方法。

It has the potential for the application of screening the inhibitor or activator of Dam methylase .

高通量筛选，例如微阵列和RNA干扰筛选产生了大量数据。

High-throughput screens such as microarrays and RNAi screens produce huge amounts of data .

结论：基于DNA微阵矩技术的肿瘤基因表达谱分析能够高通量筛选与肝癌发生发展相关的基因。

Conclusion : The cDNA microarray for analysis of gene expression profile is a powerful method for screening hepatic cellular carcinoma-related genes .

在新药筛选中，以SPA技术为基础的高通量筛选平台在国外已得到广泛的应用。

In high throughput drug screening , the SPA technology has been widely used overseas .

中药提取物中毒蕈胆碱M1受体激动剂的高通量筛选

High Throughput Screening of Agonist for M1 Receptor from Traditional Chinese Medicine

研究结果表明抑制性消减杂交结合cDNA微阵列用于大规模、高通量筛选血吸虫不同发育阶段的差异表达基因是可行的。

Our results indicated that it is feasible that SSH combined with cDNA microarray to isolate and identify differentially expressed genes .

细胞色素P450酶诱导和抑制效应高通量筛选系统研究进展

Advances in high-throughput screening for the induction and inhibition of cytochrome P450 activities

目的建立适用于高通量筛选的黄嘌呤氧化酶（XanthineOxidase，XO）抑制剂筛选模型，并以此模型进行XO抑制剂筛选。

OBJECTIVE To establish a high-throughput assay for xanthine oxidase inhibitor screening .

针对靶标G蛋白偶联受体从大量化合物中筛选出理想的药物，就需要开发特异且灵敏的高通量筛选体系。

In order to screen desirable drugs for the target receptors from a large amount of chemicals , high throughput screening systems with specificity and sensitivity are needed .

目的：建立以荧光共振能量转移（FRET）电压敏感探针为染料的钾通道抑制剂高通量筛选模型。

AIM : To establish high-throughput potassium channel assay using FRET-based voltage sensor probes ( VSPs ) .

以FRET技术构建的生物传感器具有高灵敏度和高特异性，成为高通量筛选（HighThroughputScreen，HTS）的最好系统之一。

Since FRET-based biosensor has much higher specificity and sensitivity , it becomes one of the best systems for High Throughput Screen ( HTS ) and proteomic application .

商业化的miRNA芯片是高通量筛选肿瘤相关miRNAs差异表达的有效方法。

Commercial miRNA microarray is an effective method for high-throughput screening differential expression miRNAs in tumors .

基于极化荧光方法的人LOX-1配体高通量筛选

Identification of ligands for human LOX-1 through fluorescence polarization-based high throughput screening

分子模拟用于HLA-A2.1限制性CTL表位的高通量筛选

The Application of Molecular Modeling in High Flux Screening of HLA-A2.1 Restricted CTL Epitopes

结论1.蛋白质组学技术可高通量筛选HBV相关的男性和女性肝癌中的差异表达蛋白，这些蛋白分子可作为潜在的分子标记物进行深入研究，有助于揭示男性肝癌高发潜在的分子机制。

Proteomics is the high-throughput technology to screen differentially expressed proteins in HBV-related male and female HCC , and the proteins could be in-depth study as the potential molecular markers . 2 .

信号标签诱变技术（STM）是一种在体内高通量筛选病原体毒力基因的新方法，在应用时的一个先决条件是要建立合适的体内筛选系统。

Signature-tagged mutagenesis ( STM ) is a novel technology with high throughput screening ability to identify virulent genes of pathogen in vivo .

本研究首次对普洱茶活性因子进行PPAR模型的高通量筛选研究。结果表明，普洱茶各粗提物对PPARα受体的激活作用很弱，醇提比水提更容易浸出激活PPAR受体的活性成分；

This paper used PPAR model of High Throughput Screening , being the first time in Pu'er Tea study , to screen active compounds of Pu'er Tea .

通过对Caspase-1的一系列酶学特性，包括DTT的影响、pH依赖性、动力学分析及阳性抑制剂抑制作用分析，优化Caspase-1的活性检测方法，建立了Caspase-1抑制剂高通量筛选的标准化操作流程。

The characteristics of active Caspase-1 were investigated , including DTT influence , pH dependence , the kinetics of enzyme reaction and inhibition by positive inhibitor .

本课题所建立的筛选模型能够有效的用于M1受体激动剂的高通量筛选和中药活性成分的分离、鉴定，是药物筛选的有效手段。

In a word , this screening model can be succeeded in screening agonist for M1 receptor in high throughput screening and identifying the bioactivity components of Traditional Chinese medicine .

本文结合作者的研究结果评述了NMR在组合化学中的应用，着重于NMR在固相合成的应用、液态NMR和NMR在高通量筛选中的应用。

Combining author 's research , the applications of nuclear magnetic resonance spectroscopy ( NMR ) to combinatorial chemistry , including applications of NMR to solid phase synthesis , analysis in solution and screening by NMR are reviewed .

首先，我们利用荧光检测技术、分子生物学、细胞生物学技术建立了基于细胞功能的高通量筛选方法，筛选并鉴定了淫羊藿Epimediumbrevicornum中的类激素作用活性成分。

First , we developed cell-based sensitive fluorescent assay for high throughput screening and identified an active component isolated from Epimedium brevicornum .

应用RNaseⅠ保护法高通量筛选小鼠胚胎的NSATs及序列分析

High-throughput Screening the NSATs in Mouse Embryo by RNase ⅰ Protection Assay and Bioinformatics Analysis of the Sequences

变性高效液相色谱分析（DHPLC）是近年来发展起来的高通量筛选DNA结构变异的一项新技术，可以高灵敏地检测突变、SNP、LOH，以及进行基因型分析等。

Denaturing high performance liquid chromatography ( DHPLC ) is a novel high-throughput technique for screening DNA variations . It can be highly sensitive to detect mutations , SNPs , LOH and make genotype analysis .

主要介绍了高通量筛选技术（HTS，Highthroughputscreening）的原理，包括非细胞相筛选、细胞相筛选和生物表型筛选及其在生命科学和药学领域中的应用以及高通量筛选技术的发展趋势。

This review mainly describes High Throughput Screening Assay , including non cell based screening , cell based screening and animal phenotype screening , and the application of these technologies in the fields of life science and pharmaceutics as well as the trend of development of HTS .

运用FTIR技术测定发酵培养后的菌体，红外数据用PCA、HCA和ANN分析，试图找到利迪链霉菌开始合成利迪链菌素时的biomarker，以及用于突变株高通量筛选的方法。

For estimating the biomarker of Streptomyces lydicus for producing streptolydigin and the method for high-throughput screening the mutant strain , FTIR was used to detect the mycelium , which were calculated for different time , and the data were analyzed by PCA , HCA and ANN .

目的应用荧光方法建立、优化、评价艾滋病病毒1型（HIV-1）蛋白酶抑制剂高通量筛选模型，并用于大规模样品筛选。

Objective HIV protease inhibitors are demonstrated to be effective drugs against the HIV infection The purpose of the present study is to establish a high throughput screening model by adopting fluorescent substrate and optimize such a system so as to be able to screen a large number of samples .

方法：在大肠杆菌中表达hMECD，并根据酶活性以比色法建立高通量筛选模型。

METHODS : Catalytic domain of human macrophage elastase was expressed in E coli and characterized to establish a high-throughput screening assay using a colorimetric method .