高免血清

目的为提高口蹄疫诊断试剂的敏感性和特异性，拓宽试剂的使用范围，用非结构蛋白研制重组抗原和高免血清，建立鉴别口蹄疫免疫动物与感染动物的竞争ELISA方法。

Object In order to enhance the specificity and sensitivity of diagnostic reagents for FMD , a recombinant antigen and hyperimmune serum were developed with NSPs ( 3ABC ) for the competitive ELISA kit to distinguish the infected animals from vaccinated and FMDV free animals .

经SDS-PAGE，成功表达出了大小约为43kDa的重组蛋白，westernblot分析结果表明与兔抗IBV高免血清有较好的反应性。

The result of SDS-PAGE showed that recombinant protein about 43 kDa were successfully expressed Western blot analysis showed that the protein can react positively with rabbit hyperimmune serum against IBV .

免疫球蛋白（IgG）从小鹅瘟高免血清中提取出来，吸附于固定的鹅红血球表面。

Immunoglobulin G ( IgG ) was extracted from the gosling pest virus antiserum , and was adsorbed on sensitized goose red blood cells .

将该病毒抗原制成油佐剂灭活疫苗，免疫SPF鸡，收集高免血清，用（NH4）2SO4法提取血清中的免疫球蛋白G（IgG），测定蛋白含量为IgG浓度为4.090mg/mL。

The SPF chickens were incubated with oil-adjuvanted inactivated AIV vaccine , then the high titer serum was collected and the Immunoglobulin G was extracted with the saturated ( NH_4 ) _2SO_4.And the content of the IgG is 4.090mg/mL .

山羊抗犬瘟热-细小病毒二联高免血清的研制

Preparation of Goat Antisera to Canine Distemper Virus and Canine Parvovirus

猪伪狂犬病高免血清的制作与初步应用

The preparation and application of pig pseudorabies virus high immune serum

兔病毒性出血症病毒高免血清的制备与应用效果

Preparation and Application Hyperimmune Serum Against Rabbit Hemorrhagic Disease Virus

仔猪水肿病高免血清的制备及初步应用

Preparation and Preliminary Application of High Titer Immuno Serum of Piglet 's Oedema Disease

犬多价高免血清的研制及应用

Preparation of canine multivalence serum and its application

山羊抗鸡新城疫、传染性法氏囊病、大肠杆菌病三联高免血清的研制与应用

The Preparation and Application of Goat Trivalent High Immune Serum Against ND 、 IBD and E.coli Disease

环磷酰胺预处理制备兔抗Ⅰ型鸭肝炎病毒高免血清

Hyperimmune Serum against Duck Hepatitis Virus Type ⅰ after Rabbit Pretreated with Normal Chicken Embryo Allantoic Fluid and Cyclophosphamide

结论：研制成稳定、高效的犬多价高免血清，并获得了较好的经济效益和社会效益。

Conclusion : Canine multivalence serum may be of great value in economics and society and contribute to curative disease .

用弓形虫血清抗体阴性的健康家兔，以4种不同的免疫程序皮下接种弓形虫速殖子裂解抗原制备高免血清。

Four different immune programs were used to inoculate hypodermically lysis antigen of Toxoplasma gondii tachyzoite and to produce high titer immune serum .

动物保护试验和田间应用试验的结果均表明，制备的高免血清和用灭活油乳剂苗免疫种鹅均能给雏鹅提供很好的被动保护。

The experimental and field applications results proved that both means of the passive immunity can provided very good prevention for gosling against GNTVE .

一株被兔抗虫卵可溶性抗原高免血清检获的基因克隆，其虫源基因片段经分子杂交证明系单拷贝基因。

The foreign schisto gene of this clone was proved to be a single copy gene which could be hybridized with fragments from the adult worm genomic DNA .

免疫印迹显示，该蛋白条带与兔抗猪肺炎支原体高免血清有阳性反应。结论Mhp168株黏附因子基因在大肠杆菌中获得了高效表达，重组蛋白具有免疫反应性。

It concludes that high efficiency expression of the adhesin gene of Mycoplasma hyopneumoniae strain 168 in E. coli is obtained , and the expressed protein possesses immunogenicity .

经琼扩试验和对流免疫电泳试验，表明所制抗原和高免血清的特异性、效价、敏感性及20份待检鸡血清的检测结果与标准抗原和标准血清的基本一致。

The assay of its specificity , sensitivity , titre and twenty sample of chick serum by AGP and convection immunoelectrophoresis was consistent with that of IBD standard antigen and serum .

采用盐析、透析的方法对所制的高免血清进行纯化，采用Folin-酚法和紫外分光光度法检测纯化前后蛋白浓度，纯化后血清蛋白浓度达到12mg/mL以上。

The serum was purified with the method of salting out and dialysis , and the protein concentrations was detected by the Folin-phenol method and ultraviolet spectrophotometry . The protein concentrations can reach 12 mg / mL after being purified .

用犬瘟热（CD）弱毒和自制的高免犬血清，建立了检测犬瘟热病毒（CDV）抗体的中和（SN）试验、间接荧光抗体技术（IFAT）和间接免疫酶染色法。

Thirty eight serum samples from dogs vaccinated with attenuated caine distemper virus ( CDV ) were detected by serum neutralization ( SN ) assay , indirect immunofluorescence antibody technique ( IFAT ) and immunohistochemical technique , respectively .