超薄切片技术

目的：探讨非生物标本的电镜样品制备及超薄切片技术。

Objective : To investigate the technology in preparation and ultramicrotomy of non-biological specimens for electron microscopy .

本实验结果表明，AFM技术如与超薄切片技术相结合，可对样品的内部结构进行研究，而不仅仅是一种表面成像工具。

Combined with the technique of ultra-thin sections , AFM is capable of studying the inner structure of sample .

采用组织超薄切片技术，电镜下观察研究昆虫棉铃虫HelicoverpaarmigeraHǖbner脂肪体细胞的超微结构，结果表明。

The ultrastructure of fat body tissue of Helicoverpa armigera H ü bner was observed under the electron microscope by means of ultra-thin tissue slice technology .

为研究鸭病毒性肠炎病毒（DEV）CH强毒株在感染鸭体内的分布和形态学发生规律，应用透射电镜和超薄切片技术对人工感染DEV的成年鸭各组织器官进行观察。

In order to study the morphogenesis and distribution of duck enteritis virus ( DEV ), tissues of ducks infected experimentally with DEV virulent strain were collected and observed by electron microscopy .

应用超薄切片技术对水泡性口炎病毒（VSV）在BHK-21细胞上的形态发生和早期发育进行了观察。

The morphopoiesis and progenesis of the vesicular stomatitis virus in baby hamster kidney cell-21 ( BHK-21 ) were observed by using electron microscope .

运用透射电镜的超薄切片技术和冰冻蚀刻复型膜技术研究了莫桑鼻给非鲫滤泡闭锁过程中印黄溶致液晶YLLC的形态变化特征。

Using ultrathin section and freeze-etching replica techniques the morphological changes of yolk lyotropic liquid crystals ( YLLQ ) during follicular atresia of Tilapia mossambica were observed with transmission electron microscope .

动物随机分组，用常规超薄切片技术制样电镜观察。

Their routine ultrathin sections were observed by electron microscope .

利用透射电镜超薄切片技术对病毒进行形态学鉴定。

Morphology of CCHF virus was identified by transmission electron m icrosco-py .

培养细胞的超薄切片技术探讨

Study on ultrathin sectioning technique of the culture cell

4种竹叶超薄切片技术的研究

Study on the technique of ultra-thin sectioning for four kinds of leaves of bamboo

进一步利用电镜制样技术和超薄切片技术在透射电子显微镜下观察两种处理的水稻根尖细胞中膜泡的分布和形态。

The subsection and shape of the membrane vesicles in the two managed rice roots cells were observed with the transmission electron microscope .

应用透射电镜和超薄切片技术，对长毛对虾和斑节对虾的杆状病毒病进行研究。

With transmission electron microscope and technique of ultrathin section , we studied the baculovirus diseases on Penaeus Penicillatus Alcock and Penaeus Monodon Fabricus .

方法：应用半薄切片和超薄切片技术行光镜及电镜观察大鼠直肠肛梳区的淋巴管结构。

METHODS : The lymphangial configuration was observed under a light microscope and a transmission electron microscope by using semi-thin sections and ultra-thin sections technique respectively .

应用流行病学调查方法和电镜超薄切片技术，对福建省4个中华鳖养殖场发生的病毒性传染病进行了观察。

One viral disease of Trionyx sinensis had been investigated in 4 culture regions in Fujian province by using epidemiological investigation and ultrathin section of the transmission electron microscope .

利用直接碳复型和超薄切片技术，在透射电镜下观察了我国与种（隶28属、15科）阔叶树材的纹孔超微构造。

An electron microscopic study was undertaken on the pit structure of 15 families , 28 genera and 45 spelles in hardwoods of China using ultrathin section and direct carbon replicas .

目前，种植体与软组织附着的确切机制尚无定论，口腔粘膜基层上皮细胞纯化培养及种植体组织界面超薄切片技术的困难是影响这项研究的两个难题。

Difficulties in culturing purified oral mucosal basal cells and in preparing ultra - thin sections of the tissue / implant interface are two obstacles to the research in this field .

本文用超薄切片电镜技术对水痘一带状疱疹病毒（VZV）分离株J1的核衣壳进行了形态学研究。

The nucleocapsids of varicella-zoster virus ( VZV ) strain J1 isolated hy Dr. Hun Shi-jie et al in Jinan in 1990 were studied with electron microscope .

采用非树脂包进埋去包埋超薄切片电镜技术，并结合选择性生化抽提方法以及间接免疫荧光染色方法显示，在四膜虫（Tetrahymenathermophila）细胞内存在典型的核纤层结构。

The nuclear lamina in the nuclei of Tetrahymena thermophila was investigated using the techniques of selective extraction and embedment-free electron microscopy , as well as immunofluorescence staining .

应用超薄切片电镜技术结合精子活动轨迹摄片法研究了十二种甘油-蔗糖不同配伍的冷冻保护剂冻存精子的超微结构及活动力。

The motility and ultrastructure of frozen-thawed human spermatozoa , which were protected by 12 different cryoprotective media composed of glycerol and sucrose , were studied by means of ultrathin-section TEM technique and photomicrograph method .

为阐明水痘-带状疱疹病毒济南分离株（VZVJ1）在兔脑神经细胞（RNC）中的形态与形态发生特征，我们利用超薄切片电子显微镜技术对感染VZVJ1的RNC进行了观察研究。

To Investigate the morphology and morphogenesis of the isolated varicella zoster virus ( VZV ) in rabbit brain nerve cell ( RNC ), ultrathin section electron microscopy was used for study . A small number of nucleocapsids were observed in the nuclei of RNC at 6 h post infection .

应用超薄切片透射电镜技术在实验Balb/C小鼠外周血浆细胞中意外地检出逆转录病毒。

Retrovirus was detected unexpectedly from the plasma cell of peripheral blood of experimental Balb / C mice which were immunized by anthrax vaccine .

方法：应用超薄切片和透射电镜技术，比较N株和RC株虫体食物泡和疟色素形态及形成的差异。

Methods : The RC strain and the N strain were compared concerning the formation and morphologic features of digestive vacuoles and haemozoin with transmission electron microscope .

本研究运用包埋、超薄切片和电子显微镜技术研究了EpNPV的形态特征和病理特征；

Utilizing such techniques as embed , super-thin slice , transmission and scanning electron microscope to conduct the studies on the morphology and pathology of EpNPV ;

微型振荡器在电镜超薄切片样品制备技术中的应用研究

The Application of Mini-Vibrator in Preparation of Ultra-thin Section Specimen

方法应用电子显微镜，采用超薄切片和冷冻蚀刻技术观察9例DMD/BMD患者超微结构的改变。

Methods The ultrastucture of muscles in 9 patients with DMD / BMD was observed under electronic microscopy using ultrathin section and fre eze-etching technique .