萤光素酶

TNFα（100～10000U/ml）可明显抑制萤光素酶表达至对照的0.58倍，也呈剂量依赖型。

TNF α ( 100 ~ 10000 U / ml ) induced a pronounced dose dependent inhibition of luciferase expression , down to 0.58 fold of the control .

用分泌型萤光素酶报告系统比较TTR启动子与CMV启动子的体内外表达特性

Comparison of TTR and CMV Promoters in vivo and in vitro via a Secreted Luciferase Reporter System

铅处理组萤光素酶活性较空白对照组高（P0.01），且具有剂量依赖性；

It is also found that the luciferase activity of groups treated with lead acetate are higher than that of blank control group ( P0.01 ) with a dose-effect relationship .

采用双萤光素酶实验检测过表达IGFBP-3对甲状腺素T3激活的生长激素启动子的影响。

The effect of IGFBP-3 on the growth hormone promoter activity stimulated by triiodothyronine ( T3 ) was determined by dual-luciferase reporter assay .

应用萤光素酶报告基因系统证明了本实验所鉴定的NF-κB反应元件是nNOS1f启动子中一个强大的正调控元件，同时它在TSA引起的nNOS1f启动子的转录激活中发挥重要作用。

Results of luciferase assay showed the identified NF - κ B responsive element acted as a powerful positive regulation element and took an important part in TSA-induced activation of nNOS 1f promoter . 7 .

结果构建并鉴定了系列截短的NOS1启动子萤光素酶报告基因载体；

Results A series of truncated NOS1 promoter luciferase reporter vectors were constructed and identified .

铅+PDTC联合作用组与PDTC对照组相比，PC12细胞内萤光素酶活性增高（P<0.01）。

Significantly higher luciferase activity are found in the groups treated with lead acetate and PDTC compared with the PDTC control group ( P < 0.01 ) .

用不同浓度的醋酸铅处理转染后的PC12细胞24小时，结果显示：PDTC对照组萤光素酶活性较空白对照组降低（P<0.01）；

The results showed that luciferase activity of PDTC control groups is lower than that of blank control group ( P < 0.01 ) 24 hours after lead acetate exposure .

将环状糊精糖基转移酶的SBD基因编码序列连接到萤光素酶基因的5′端（SBDLUC）后，重组基因通过农杆菌介导导入马铃薯植株中。

The starch-binding domain ( SBD ) - encoding region of cyclodextrin glycosyltransferase from Bacillus circulans is fused to the 5 ′ - terminal end of the luciferase ( LUC ) gene , via an artificial Pro-Thr encoding linker sequence .

甘露碱合成酶基因启动子调控的萤光素酶基因在转化烟草中的表达

Mannopine Synthase Promoter Directed the Firefly Luciferase Gene Expression in Transgenic Tobacco

萤火虫萤光素酶基因构建BIV－LTR启动子表达研究体系

Construction of BIV - ltr promoter expression system with firefly luciferase gene

带新霉素抗性的萤光素酶报告载体的构建

Construction of a luciferase reporter vector with resistance of neomycin

萤火虫萤光素酶-萤光素分子体系的生物荧光现象研究生物发光是自然界常见的现象，其中以萤火虫发光效率最高。

Study of firefly luciferase-luciferin bioluminescence system Bioluminescence is a quite common natural phenomenon .

邦帕的研究团队一直在研究通过转基因细菌提炼的萤光素酶制作在黑暗中发光的甜点的方法。

The team at Bompas have since been developing further glow in the dark desserts using luciferin enzymes from genetically modified bacteria .

软件分析发现nNOS1f启动子内存在一些潜在的受乙酰化修饰的转录因子结合位点；萤光素酶报告基因检测在启动子内发现两个正调控区和两个负调控区。

Software analysis suggested a number of putative responsive elements of transcription factors that are subject to acetylation within nNOS If promoter . Luciferase assay indicated two positive and two negative regulatory regions . 4 .

应用软件分析nNOS1f启动子结构；并构建系列截短的nNOS1f启动子萤光素酶报告基因载体，应用双萤光素酶检测系统分析各段启动子的活性。

NNOS 1f promoter structure was analyzed by softwares . A series of truncated luciferase reporter vectors were constructed and Dual-Glo luciferase assay system was used to detect the activity of different parts of the promoter . 4 .