菌蛋白

在红霉素诱导下，共得到3个差异表达的蛋白溶藻弧菌全菌蛋白，分别是磷酸烯醇丙酮酸羧激酶、外膜蛋白N和磷酸丙糖异构酶。

There were 3 spots differentially expressed of V. alginolyticus induced by erythromycin . The 3 protein pots are triosephosphate isomerase and outer membrane pro - tein N and phosphoenolpyruvate carboxykinase .

SDS-PAGE蛋白电泳后，各泳道酶蛋白量多少依次为包涵体蛋白、全菌蛋白以及培养液上清，提示转化子表达产物主要存在于包涵体内。

The SDS-PAGE electrophoresis results showed that the expressing products quantity was supernatant , whole bacterial proteins and inclusion body protein in turn .

重组质粒分别转染COS7细胞和293细胞，可溶性表达PCP2EC/Fc融合蛋白，并通过金葡菌蛋白A特异性纯化；

PCP-2EC / Fc fusion protein was expressed by COS-7 and 293 cells transfected by the constructed plasmid and purified by protein A.

利用酿造糟生产菌蛋白饲料的研究（中试）

Development of Fungus Protein Feed Fermented From Brew Dregs ( Middle-sized experiment )

采用水煮裂解法，完成全菌蛋白提取。

The bacterial protein was extracted by water boiling extraction technique . 3 .

革兰氏阴性菌蛋白分泌系统研究进展

Research Advance of Protein Secretion Systems of Gram-negative Bacteria

该小组指出菌蛋白并不是一种蘑菇（一种普通的食物）。

It noted that mycoprotein is not a mushroom , which is a common food .

空肠弯曲菌蛋白抗原分析

Analysis of Protein Antigens of Campylobacter jejuni

研究了水分和营养盐对甜高粱茎秆固态法酒精发酵的影响和固态发酵联产混菌蛋白饲料工艺的可行性。

The results show that the solid-state fermentation of herb residues by Trichoderma koningii to produce protein feedstuff is feasible .

经薄层扫描分析，目的蛋白条带占全菌蛋白的38.4%。（4）表达后菌体超声破碎后，目的蛋白主要存在于上清中。

The aim protein representing 38.4 % of total cell protein by scanning map analysis ( 4 ) Most of the aim protein is in supernatant after ultrasonic crash .

本研究利用免疫试管凝集法，聚丙烯酰胺凝胶电泳法，免疫印迹法，荧光抗体法，酶联免疫吸附法等对6种病原性细菌的全菌蛋白进行了结构和免疫特性的分析。

With Immediate Agglutination , SDS-PAGE , Western-blot , IFAT and ELISA methods , the analyses were carried out on structures and immunological characteristics of the whole proteins possessed by the 6 bacteria mentioned above .

B群脑膜炎奈瑟氏菌外膜蛋白抗原性分析

Analysis of the OMP antigenicity of serogroup B meningococci

类产碱假单胞菌杀虫蛋白的N端鉴定及抗体制备

N-terminal analysis and antibody preparation of insecticidal protein form Pseudomonas pseudoalcaligenes

淋病奈瑟菌孔蛋白B原核重组质粒的构建、表达与蛋白鉴定

Construction of Neisseria gonorrhoeae Porin B Plasmid Recombinant and Its Expression in E.coli and Identification of Fusion Protein

幽门螺杆菌鞭毛蛋白B亚单位原核表达系统的构建及其产物的免疫性

Construction of Prokaryotic Expression System of Helicobacter pylori Flagellin Subunit B Gene and Immunological Study of Expressed Product

基于黄单胞菌冰核蛋白N端表面展示体系的建立及初步应用研究

Establishment of Cell Surface Display System Based on INP N-domain of Xanthomonas and Its Preliminary Application

结论淋病奈瑟菌孔蛋白PⅠB原核表达质粒构建正确。

Conclusion A plasmid recombinant encoding porin B of Neisseria gonorrhoeae has been successfully constructed and expressed in E. coli .

大豆立枯丝核菌G蛋白β亚基基因的克隆与分析

Cloning and Analyzing of G-protein β - Subunit Gene in Rhizoctonia solani Causing Soybean Sharp Eyespot

鼠伤寒沙门氏菌外膜蛋白诱导BALB／C小鼠免疫保护的实验研究

Study on protective immunity induced balb / c by outer membrane proteins of Salmonella typhimurium

升温诱导表达后，每升培养液大约可得到菌体湿重3g，其中目的蛋白约占菌体总蛋白量的40%。

3g cell wet weight per liter of culture broth was obtained .

幽门螺杆菌外膜蛋白对HelicobacterPylori感染的诊断

Helicobacter pylori outer membrane proteins in diagnosis of Helicobacter pylori infection

结果带有pBV220/hPK5的大肠杆菌表达的目的蛋白占菌体总蛋白的348%，其表达蛋白具有Histag抗原活性和野生活性。

Results The expressed product contained 34.8 % of total somatic protein and showed His-tag antigen activity .

八株弯曲菌外膜蛋白的SDS-PAGE分析

Analysis of Outer Membrane Proteins From Eight Strains of Campylobacter in SDS-PAGE

所表达的E蛋白的相对分子质量约为50000，表达量约占菌体总蛋白的35%。

The yield of expressed JEV E protein with relative molecular mass approximately 52 × 10 3 was 35 % of total bacterial proteins .

空肠弯曲菌外膜蛋白SDS-PAGE应用于流行病学调查的研究

Studies of Outer Membrane Protein Profiles by SDS-PAGE for Campylobacter jejuni in an Epidemiological Investigation

幽门螺杆菌依赖蛋白酪氨酸激酶途径诱导胃上皮细胞分泌IL-8

Helicobacter pylori-induced interleukin 8 secretion by gastric epithelial cells its relation to protein tyrosine kinase activation

结果大肠杆菌中表达的h－EraC端结构域蛋白和（His）6－h－ERA融合蛋白，分别占菌体总蛋白的40%和80%。

Results The expressed h-ERA C-terminal domain and ( His ) 6 - h-ERA fusion protein occupied about 40 % and 80 % of total bacterial protein respectively .

重组菌中蛋白的表达及表达蛋白的定位通过SDS-PAGE、WESTERNBLOTTING和间接免疫荧光进行鉴定。

The expression and localization of the protein from recombinant strains were detected via SDS-PAGE , Western blotting and indirect immunofluorescence .

福氏2a志贺菌MarA蛋白纯化复性及活性检测

Renaturation , purification and identification for biological activity of shigella flexneri 2a MarA protein

鸡沙门氏菌外膜蛋白亚单位疫苗的研究&Ⅱ油乳剂型（OE）亚单位疫苗的研究

Studies on OMP Subunit Vaccine of Chicken Salmonella & II Studies on Oil Emulsion Subunit Vaccine