茜素红

用维生素C、β-磷酸甘油、地塞米松诱导10T1/2细胞成骨分化，第21天茜素红染色可见红色骨结节。

After induced to osteoblast differentiation by combining ascorbic acid ,β - glycerophosphate , and dexamethasone , bone-like nodules were identified by alizarin red staining at day 21 .

DPSCs-MAO组与BMSCs-MAO组钙结节茜素红染色阳性，钙结节面积差别无统计学意义（P0.05）。

The alizarin red positive mineralized area in DPSCs - MAO and BMSCs - MAO were no significant difference ( P0.05 ) .

多目标单纯形法在茜素红催化荧光测定痕量钒（V）中的应用

Application of Multi-object Simplex Method in Catalytic Fluorimetric Determination of Trace Vanadium ( V ) Using Alizarin Red

茜素红-H2O2-溴代十六烷基吡啶-丙氨酸体系催化光度法测定痕量锰

Alizarin Red-H_2O_2-Cetyl Pyridine Bromide ( CPB ) - Alanine System for Spectrophotometric Determination of Trace Manganese

茜素红与蛋白质分子Langmuir吸附聚集反应研究

Langmuir aggregation of alizarin red in protein macromolecule

应用碱性磷酸酶（ALP）检测试剂盒及茜素红法检测细胞ALP比活性和细胞矿化能力；

Cellular alkaline activity and mineralization were examined by ALP measurement kit and Alizarin Red-S staining .

茜素红染色、钙钴法染色、四环素荧光标记等显示SW组细胞的成骨作用明显优于对照组；

It was showed that osteogenesis was clear in SW group by histochemistry and tetracycline fluorescence staining .

本文提出了Cu（Ⅱ）－茜素红－罗丹明B－聚乙烯醇三元配合物体系光度法测定Cu（Ⅱ）的新方法。

The new spectrophotometric method to determine Cu (ⅱ) under the ternary complex system of Cu (ⅱ) - Alizarin Red - Rhodamine B - Polyethylene Glycols is presented in the paper .

Mn~（2+）/H2O2光催化体系对染料甲基橙、茜素红、结晶紫的降解研究

Study on Degradation of Methyl Orange , Alizarine Red and Crystal Violet Using the Photocatalytic System of Mn ~ ( 2 + ) / H_2O_2

碳酸盐对Cr（Ⅲ）-茜素红S显色体系的慢反应在微酸性条件下具有显著的催化作用，据此，提出了一种测定微量碳酸盐的动力学新方法.并确定了最佳测定条件。

A catalytic spectrophotometric method for the determination of trace carbonate is proposed based on the catalytic effect of carbonate upon the Cr ( ⅲ ) - ARS reaction .

Fe~（2+）/Mn~（2+）/H2O2体系对含茜素红染料废水的脱色与降解处理

Decolorization and degradation of dye wastewater containing Alizarine Red by fe ~ ( 2 + ) / mn ~ ( 2 + ) / h_2o_2 system

茜素红S、四环素荧光标记钙结节形成情况。RT-PCR检测骨钙素mRNA的表达。

The formation of calcium nodules were confirmed by Alizarin red S and tetracycline fluorescence staining . The expression of bone gla protein mRNA was examined by RT-PCR .

建立了以茜素红（AR）为电活性探针方波伏安法间接测定无电活性的壳聚糖（CS）的新方法。

A new method to indirectly determine chitosan ( CS ) was established by using alizarin red ( AR ) as an electrochemical activity probe and square wave voltammetry .

方法采用细胞培养、MTT比色测定、碱性磷酸酶测定法、考马斯亮蓝法及茜素红染色法。

Methods Using cell culture technique , MTT colorimetric assay , ALP activity assay , Commasie brilliant blue staining and Dahl McGec Russell 's alizarin red stain for calcium .

应用MTT法，对硝基苯磷酸盐法（PNPP）及茜素红染色方法观察补肾活血方对体外培养成骨细胞的增殖、碱性磷酸酶（ALP）活性及矿化结节形成的影响。

MTT , PNPP and ARS were used to observe the proliferation , activity of ALP and the number of mineral node of cultured osteoblasts in vitro .

研究了在pH8.5的NaB4O7-HCl缓冲溶液中，Fe（Ⅲ）催化H2O2氧化茜素红褪色的反应可被痕量EDTA有效地阻抑，被过量EDTA有效的终止，据此建立了动力学光度法测定痕量EDTA的新方法。

In NaB_4O_7-HCl solution , the catalytic action of Fe (ⅲ) on the oxidation of alizarin red by H_2O_2 was effectively inhibited by EDTA . The reaction was effectively stopped by superfluous EDTA .

术后6h应用锥蓝-茜素红活性染色，光镜下观察各组角膜内皮细胞微细结构的变化。

At 6 hours postoperatively , trypan blue-alizarin red active staining was performed , and the changes of slight structures of corneal endothelium were observed under light microscope .

采用酶消化联合组织块培养法分离的胎鼠头盖骨成骨细胞体外连续培养最多达到13d，AKP和茜素红S染色阳性均表现阳性结果。

Osteoblast were isolated from fetal mouse cranial bone with method which based on combination of tissue block and mixed enzyme digestion .

通过茜素红（ARS）染色，MTT法及其他组织化学的方法来研究在生物反应器中培养14d时的成骨潜能。

The osteogenic potential of the cell-CHA scaffold constructs cultured in bioreactor were studied by Alizarin Red S ( ARS ) staining , MTT method and histochemistry at the 14th days .

成脂、成骨诱导培养21d分别用油红O及茜素红染色均呈阳性。

The results of staining with Oil red O and alizarin red S are both positive after 21 days adipose and osteogenesis inducing culture .

采用β-甘油磷酸钠、维生素C、地塞米松联合诱导，使胎盘贴壁细胞分化为成骨细胞，诱导后10d行茜素红染色；

Cells of 3 passages were induced to differentiate into osteoblasts with vitamin C , dexamethasone and sodium β - glycerophosphate , and the induced cells were observed in the change of matrix mineralization with alizarin red staining ;

利用阿利新蓝及茜素红双染色技术研究不同发育时期的蝌蚪暴露于不同浓度的NaF中，对骨骼发育的影响。

The double-stained method with alcian blue and alizarin red were used to investigate the skeletal development of the larvae exposed to different concentrations of NaF at different development stage .

成骨诱导后进行ALP、茜素红、VOnKossa染色结果均为阳性，通过免疫荧光和RT-PCR检测，ALP、OPN、OCN和ONN均有表达。

The ALP , Alizarin red , Von Kossa staining results are positive after Osteogenic induction . by immunofluorescence and RT-PCR detection , ALP , OPN , OCN , and ONN are expressed .

骨诱导培养后，实验组的ALP染色、VOnKossa染色及茜素红染色均呈现阳性，而对照组不被染色或染色不明显。

After bone induction , the experimental group was tested positive while the control group was not stained or was not apparently stained by ALP staining , von Kossa staining and alizarin red staining .

方法：制备补肾健脾活血方复方中药含药血清，取不同浓度剂量干预体外培养的成骨细胞，用MTT法检测成骨细胞增殖率，用茜素红染色法显示矿化结节的形成。

Methods : made the medicated sera of Bushen Jianpi Huoxue Recipe , used different density dosage to interfere the cultured osteoblasts , detected the proliferation rate of osteoblasts with MTT assay , showed the formation of mineralization tuberculums with alizarin Bordeaux staining .

贴壁细胞在成骨诱导剂作用下，碱性磷酸酶（ALP）水平升高，ALP钙钴染色呈阳性反应，钙结节茜素红染色反应阳性。

It was clarified that the cultured cells were originated from stem cells . The adherent cultured cells increased the activity of ALP , ALP staining reaction and calcium nodus alizarin red staining reaction were positive with the osteogenic inducer .

于妊娠第20d处死孕鼠，将胎仔茜素红和阿利新蓝骨骼双染后，检查全身骨骼发育程度。

All animals were sacrificed on day 20 of pregnancy . After double stained with alizarin red S and alcian blue , the rat fetal skeleton was examined .

诱导后的成骨细胞应用细胞形态学、细胞活力检测、细胞生长及增殖曲线、ALP活性检测、矿化结节茜素红染色、I型胶原免疫组化与ALP鉴定。

Osteoblasts derived from BMSCs were verified by cell morphology , cell vigor detection , cell growth curve , ALP activity examination , alizarin Bordeaux dyeing of mineralization nodes , immunohistochemistry of collagen I and Gomori dyeing of ALP . 3 .

在成骨诱导液中分别培养14天、21天后，对支架上的细胞进行碱性磷酸酶（ALP）和茜素红染色，其结果呈阳性，表明hMSCs被成功诱导成了成骨细胞。

After induced in the osteogenesis inducing solution ( OS ) for 14d and 21d separately , the results of alkaline phosphatase and alizarin red dyeing were positive , which meant that hMSCs had turned into osteoblasts .

传代后第7d即可汇合，第14d出现矿化结节，茜素红染色呈橘红色，VOnKossa染色呈黑色；

The merging osteoblasts were found from passage culture on day 7 , and there were apparent mineralized nodules on day 14 . Von kossa and Alizarin red staining showed positive with jacinth and black respectively .