脱饱和酶

被孢霉Δ～9脂肪酸脱饱和酶cDNA序列的克隆与鉴定

Cloning and Characterization of cDNA Encoding Δ~ 9 Fatty Acid Desaturase of Mortierella

被孢霉cDNA文库的构建及△~9脂肪酸脱饱和酶cDNA序列的筛选

Construction of cDNA library of Mortierella and screening of △ ~ 9 fatty acid desaturase cDNA sequence

通过RT-PCR及cDNA末端扩增（RapidAmplificationofcDNAends，RACE），获取△~4-脂肪酸脱饱和酶cDNA的全长基因，序列分析显示其开放阅读框由1560个核苷酸组成，共编码519个氨基酸；

And the full-length cDNA of delta-4 fatty acid desaturase was obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ), its whole ORF is 1560 bp encoding 519 amino acids ;

通过对已克隆△~9脂肪酸脱饱和酶基因的分析，合成一组简并引物，PCR扩增了被饱霉△9脂肪酸脱饱和酶基因的保守区。

Conserved region of △ 9 fatty acid desaturase gene from Mortierella was cloned and sequenced through PCR using one group of degenerate primers designed on the basis of analysis of cloned A ' fatty acid desaturase Sequences .

芸薹属作物油酸脱饱和酶基因（FAD2）拷贝数和序列变异分析

Sequence and Copy Number Variation of Oleate Desaturase Gene ( FAD2 ) in Brassica Species

植物中的△9硬脂酰-ACP脱饱和酶（Stearoyl-ACPdesaturase，SAD）催化在第9-10碳原子之间脱氢形成脂肪酸的第一个双键，直接决定植物储脂中不饱和脂肪酸的比例。

Plants △ 9 stearoyl-ACP desaturase enzyme ( SAD ) catalyzed the dehydrogenation reaction between 9-10 carbon atoms and formation the first double bondis . Therefore they directly determined the ratio of unsaturated fatty acid in storage .

特别强调运用高新生物技术如突变、基因工程、脱饱和酶抑制剂、酶促生物转化等技术来增加新的PUFA种类，提高其产品价值，从而使其能与其他来源的PUFA相竞争。

The new biological techniques ( for example mutation methods , genetic engineering , the use of desaturase inhibitors and enzymatic biotransformations ) were emphasized to increase new species of PUFA and improve its value in order to compete with PUFA from other sources .

在油脂合成过程中，乙酰辅酶A酰基转移酶（AACT）催化蛋白质的酰基化和去酰基化，而脂肪酸脱饱和酶6（FAD6）则控制油酸脱氢形成亚油酸和亚麻酸等多不饱和脂肪酸。

In the process of the oil combining . AACT is an enzyme that catalyzes the formation of acetoacetyl-CoA from two molecules of acetyl-COA . Fatty acid desaturase catalyze oleic acid into linoleic acid , linolenic acid and by dehydrogenation .

深黄被孢霉的脱饱和酶活性与花生四烯酸产量的关系

The Relation between Desaturases Activity in Mortierella Isabellina and ARA Contents

△~6-脂肪酸脱饱和酶基因种子特异表达载体的构建

Construction of Seed-specific Expression Vector of Δ ~ 6-Fatty Acid Desaturase Gene

农杆菌介导反义油酸脱饱和酶基因转化甘蓝型油菜

Introduction of Trans Desaturase Gene into Brassica napus via Agrobacterium Mediated Transformation

小克银汉霉发酵产γ-亚麻酸及Δ6脱饱和酶基因克隆

Gamma Linolenic Acid Production by Cunninghamella Sp and Cloning of △ 6 Desaturase Gene

脂肪酸脱饱和酶的研究进展

Progresses in Study of Fatty Acid Desaturase

高等植物中的△12脂肪酸脱饱和酶是将油酸转化为亚油酸的酶。

Delta-12 desaturases are involved in the con-version of oleic acid to linoleic acid in plant .

三角褐指藻Δ~6脂肪酸脱饱和酶基因克隆及其表达分析

Cloning and Expression of Δ ~ 6-Fatty Acid Desaturase Gene from Phaeodactylum tricornutum in Pichia pastoris