羊水细胞培养

目的：探讨羊水细胞培养及荧光原位杂交（FISH）技术在产前诊断中的实验方法及应用价值。

Objective : To study the experimental method of amniotic cell culture with fluores-cence in situ hybrydization ( FISH ) for prenatal diagnosis and its application value .

对252例适应症对象进行改良羊水细胞培养产前诊断，取得满意的成功率，并检出DS胎儿3例。

As a technique of prenatal diagnosis , modified amniotic cell culture was successfully undertaken in 252 patients , 3 cases of them were identified with DS .

结论羊水细胞培养进行产前诊断是十分安全而可靠的。

Conclusion amniotic fluid culture for prenatal diagnosis is very safe and reliable .

方法对我院优生遗传咨询门诊有产前诊断指征的孕妇采用羊水细胞培养G显带技术进行羊水细胞培养染色体核型分析。

Methods : G banding technique was used on cultured amniotic cells .

方法：对342例具有产前诊断指征高风险孕妇抽取孕中期羊水细胞培养，制备中期细胞染色体，G显带分析。

Methods Amniocentesis was employed to detect amniotic cells collected from 342 pregnant women with high-risk prenatal diagnosis indications .

羊水细胞培养失败1例，FISH检测为1正常男胎，排除了唐筛高危，与核型分析结果一致。

One failed case , at the first time of the amniotic fluid cell cultured , FISH had a successfully detection and it was a normal male fetus , which excluding tang high-risk screening .

采用改良的羊水细胞培养方法，各种染色体显带技术，羊水细胞培养成功率大大提高，成功率为99.5%。进一步缩短了培养时间，平均7.6d。

Using the improved method of amniotic fluid cultivation and various techniques of chromosome banding , the rate of success was greatly increased , approaching 99.5 % , and the duration of culturing much shortened , averaging 7.6d .

羊水细胞培养诊断染色体病

Amniotic Fluid Cell Culture in Prenatal Diagnosis of Chromosomal Abnormalities

70例羊水细胞培养

Brief Summary of Studies on Amniotic Fluid Cell Culture from 70 Cases

羊水细胞培养与染色体制片技术探讨

On the Procedures of Amniotic - fluid Cell Culture and Chromosome Preparation

羊水细胞培养技术研究

A study on the technique of amniotic fluid cell culture

羊水细胞培养预测胎儿异常112例分析

An analysis of amniotic cell culture in 112 cases to predict fetal anomalies

方法探索新的羊水细胞培养技术；建立收获标准及改良收获方法。

Methods : To study the new technical methods and establish the standard of harvest .

羊水细胞培养成功214例，成功率91.45%；

214 cases amniotic fluid cells culture are successful , yield rate was 91.45 % ;

遗传疾病的产前诊断&Ⅰ.羊水细胞培养和产前诊断一百例结果报道

Prenatal diagnosis of human genetic diseases ⅰ . report on results of diagnosis of 100 pregnancies amniotic fluid culture

在高危孕妇中4例羊水细胞培养胎儿染色体异常，1例B超诊断为胎儿脑积水。

Cases were chromosome abnormity by amnionic fluid chromosome test , 1 case was hydrocephalus diagnosed by ultrasonic examination .

目的探讨用羊水细胞培养对孕16w~22w孕妇进行产前诊断的必要性及安全性，防止染色体病患儿出生。

Objective : To explore the necessity and security of prenatal diagnosis with amniotic cell culture at 16 to 22 week gestation and to prevent the birth of chromosomal abnormal fetuses .

提高羊水细胞染色体培养成功率研究

Investigation on improving the cultural success rate of amniotic fluid cell chromosome

孕中期羊水中细胞培养和核型鉴定广泛应用于产前诊断领域，可以发现一系列胎儿发育异常。

Amniotic fluid cells are used for prenatal diagnosis of a wide rang of fetal abnormalities caused by genetic mutations .

1例间期细胞FISH与羊水培养结果一致，另1例羊水细胞培养仅收到很少分裂中期染色体，改做FISH后可见到较好的荧光信号。

The result from one case with interphase cells by FISH was coincidence with it by amniotic cell culture . The other case got a little metaphase chromosomes by amniotic cell culture , and then got better signals in interphase cells by FISH .

对105例产前诊断对象作了羊水成活细胞和成活率分析，并采用改良的羊水细胞培养技术，进行染色体病产前诊断。

Viable cell analysis and prenatal chromosomal diagnosis with improved technique of amniotic fluid cell culture were performed in 105 cases .