纯培养

松乳菇菌丝纯培养及其分离物的DNA指纹研究

Studies on the Isolation , Pure Culture and DNA Identification of Mycelia of Lactarius Deliciosus

利用甲醇、H2+CO2或乙酸盐作为碳源和能源，从池塘的沉积物取样，经过反复的富集和分离，得到了甲烷八叠球菌的纯培养物。

Samples from pond sediments were inoculated to obtain the pure culture of Methanosarcina sp. by repeated enriching and isolating . Growth was fasted on H_2 + CO_2 or methanol .

与纯培养相比共培养物中乳酸积累的量差异不显著，少于1mM（P0.05）。

The content of lactate in both co-culture and monoculture had no significant differences ( P0.05 ) .

菌的生长有很大的影响，经过纯培养试验得出：细菌生长繁殖的最佳温度为30℃，最佳pH值为2.0。

The purification cultivation experiments have shown that the bacteria grow best when the temperature is 30 ℃ and the pH value is 2.0 . The taming cultivation experiments of T.

采用改良培养基分离Hp时间缩短近24h，纯培养时间缩短近12h，且细菌各项检测指标典型。

The time for pure cultivation could shorten 12 h , isolation shorten 24 h. All the test indexes were typically .

纯培养研究进一步证实，一些异化Fe（III）还原微生物可以利用芳香化合物作为电子供体，促进芳香族碳水化合物的氧化过程。

The study of pure culture illuminate that some microbes of Fe ( III ) reduction can utilize aromatic compounds as electron donors , and stimulate the oxidation of aromatic compounds .

方法取生后2d的Wistar大鼠的大脑皮层进行星形胶质细胞纯培养，分别予不同程度的高渗培养液作用于星形胶质细胞，建立星形胶质细胞对高渗液反应的实验模型。

Methods Rat cerebral cortical astrocytes obtained from 2-day newborn Wistar rat underwent the pure culture and its model of cell dehydration was established by exposure to hypertonic medium .

比较子实体及其组织分离菌株的rDNAITS区段序列差异，判定分离菌株为其子实体的纯培养菌株。

The isolated strains from tissue were identified as their own pure culture by comparing the rDNA-ITS sequences differences of fruiting body and isolated strains .

对14株不同宿主来源的Frankia纯培养菌株进行了形态观察和固氮活性测定。

Nitrogen fixation activities and morphological characteristics of 14 Frankia strains isolated from different host plants were determined .

将分离出的1602株支原体经三次传代成纯培养物后接种于液体培养基，置烛缸37℃培养24～48h，检测其耐药性。

After three-passage cultivation , the pure-culture was inoculated to fluid medium , cultured for 24 - 48h at 37 ℃ . Antibiotic-resistance of the isolate was studied .

外生菌根菌白毒伞（AmanitaVerna）菌丝体纯培养条件灰树花菌丝体多糖的分离纯化和理化性质研究

Study on Pure Culture of Ectomycorrhizal Fungi Amanita Verna Separation , Purification and Characterization of Polysaccharide from Grifola frondosa

Koch早就认识到发展简单的细菌纯培养技术是这门新科学成长的要害。

Koch realized very early that the development of simple methods for obtaining pure cultures of bacteria was a vital requirement for the growth of the new science .

采集并组织分离到假褐云斑鹅膏Amanitapseudoporphyria的纯培养菌丝。

Sporocarps of Amanita pseudoporphyria was collected and the pure culture was obtained by way of tissue culture .

该方法的检测敏感度为细菌纯培养物为104CFU/ml，含菌3-4CFU/g鸡肉组织样品经预增菌8h后能检出。

Sensitivity of the assay was 104 CFU / ml of bacteria samples and 3-4 CFU / g of chicken meat that underwent enrichment culturing for 8 hours .

本文介绍了Methanobacteriumruminantium纯培养物在甲烷发酵中的作用。

This paper introduces the role of methanobacterium ruminantium pure culture in the ferment of methane .

用聚合酶链式反应（PCR）扩增hly基因，检测单核细胞增生症李斯特菌的纯培养物及其模拟污染的生猪肉、水和牛奶。

A polymerase chain reaction ( PCR ) assay targeting the gene encoding hly was developed for detecting Listeria monocytogenes in pure cell cultures and on artificially contaminated water , milk and pork .

鸡肉肉水、增菌液和纯培养菌落的PCR鉴定结果，阳性率分别为16%、24%和20%，生化鉴定检出率为20%。

Chicken meat water , enrichment fluid and colony from the pure culture were detected by PCR , and 16 % , 24 % , 20 % of them were found respectively to be positive for C. jejuni . The detection rate of biochemistry identification is 20 % .

在MMN培养基上，用5&40℃范围内的15种不同温度，对云南省不同针叶林下38种常见外生菌根真菌的44个菌菌株进行纯培养试验。

44 isolates of ectomycorrhizal fungi comprising 38 species were cultured on MMN medium at 15 temperature gradients from 5 ℃ to 40 ℃ for 2 weeks .

从菠菜（Spinaciaoleracea）根瘤上分离到一种细菌内生菌84G，获得纯培养。

A bacterium endophyte 84G from root nodules of Spinacia oleracea has been isolated as pure culture . The strain is aerobic , fast-growing .

为此，我们对路南腐乳的产生菌高大毛霉（mucormucedo）进行了分离筛选及鉴定，并进行了人工纯培养。

For this reason , we have done the isolation , screening and identification for the pure culture strains-Mucor mucedo of Lunan Chinese cheese .

方法采用氨氧化细菌与亚硝酸氧化细菌富集、分离技术从武汉某啤酒厂曝气池活性污泥中分离得到2株纯培养菌株N1和N2。

Methods Two pure cultures , N 1 and N 2 , were enriched and isolated based on the procedures for ammonia oxidizer and nitrite oxidizer from activated sludge of a aeration tank in a brewery plant in Wuhan .

35S半胱氨酸代谢标记及电泳分析表明每个纯培养的虫株都有一个主要蛋白，表面标记技术展示此蛋白的大小恰好就是它的表面蛋白。

Metabolic labeling with [ 35 S ] cysteine and electrophoresis analysis also revealed for each cloned isolate a predominant protein that corresponded in size to the major surface protein demonstrated by surface labeling techniques .

抑菌实验表明，分别采用巴氏消毒法、pH<2的酸性条件和2%的NaOH处理纯培养的乳酸菌培养1h，4种乳酸菌的致死率为90%以上。（孙悟）

It would get the normal fermentation beer acidification . The experiments of inhibitary suggested that the dead rate of the four strains was above 90 % when they were treated by respectively by pasteurization , acidity condition for pH < 2 and 2 % NaOH treatment for 1 h.

对从湖北、四川、云南等地采集的尼泊尔马桑（CoriarianepalensisWall.）根瘤中分离的20株内生菌纯培养物所进行的研究表明，其形态均具有弗兰克氏菌属的特征。

Twenty strains of actinomycetes , originally isolated from nodules of Coriaria nepalensis wall in Hubei , Sichuan and Yunnan provinces of China , were shown to fall in the genus of Frankia according to the morphological characteristics .

用DAPI染色法对纯培养的菌株0503和0501进行染色并计数，结果显示，DAPI染色法计数结果与细胞计数板法和平板涂布法结果非常接近，DAPI染色的细菌背景突出，计数方便。

We used DAPI method to calculate the biomass of strain of 0503 and 0501 , the results revealed that the calculating method of DAPI and cell counting chamber was almost the same . DAPI method was very clear in which the bateria can easily distincted from the background .

外生菌根真菌纯培养中生理活性物质代谢的研究

Metabolism of physiologically active substances of ectomycorrhizal fungi in pure culture

几种纯培养外生菌根真菌对矿物油的降解效果

Degradation effect of several pure cultured ectomycorrhizal fungi on mineral oil

尼泊尔马桑根瘤内生菌纯培养物的质粒检测

Plasmid detection for pure cultures of nodular endophytes of Coriaria nepalensis

蓝氏贾第虫福建株的分离与纯培养

The Establishment of Axenic Culture of a Fujian Strain of Giardia Lamblia

根瘤菌纯培养乙炔还原的研究

The observation of acetylene reduction in pure cultures of Rhizobium